Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-04T19:01:45.814Z Has data issue: false hasContentIssue false
  • English
  • Français

Epidemiological complexity of hospital aeromonas infections revealed by electrophoretic typing of esterases

Published online by Cambridge University Press:  19 October 2009

B. Picard  and
Ph. Goullet
B. Picard
Affiliation:
Service de Microbiologie, Hôpital Beaujon (Faculté de Médecine Xavier Bichat, Universitê Paris VII), 100 boulevard du Général Leclerc, 92110 Clichy, France
Ph. Goullet
Affiliation:
Service de Microbiologie, Hôpital Beaujon (Faculté de Médecine Xavier Bichat, Universitê Paris VII), 100 boulevard du Général Leclerc, 92110 Clichy, France
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

An epidemiology analysis of a series of 12 Aeromonas hydrophila infections, including six of septicaemia, which occurred on several wards of one hospital during the summer of 1982 is presented. The hypothesis that the hospital water could be the source of these infections was supported by the isolation of 1–10 motile aeromonads per ml in most of the water samples collected from various points on the hospital water system. Electrophoretic esterase typing was used as an epidemiological screening method to determine the relationship between bacterial strains isolated from the patients and those from water samples. The epidemiology of A. hydrophila infection in the hospital was found to be complex. Amongst the 15 strains of A. hydrophila isolated from patients were 8 zymotypes, while amongst the 126 strains from the water samples there were 37. In some cases, several zymotypes were isolated simultaneously from the same tap water. On one ward, the same zymotype was found in 2 patients and in 2 water samples. The prophylactic measures taken in 1982–5 to avoid oral contamination of immunocompromised patients with infected hospital water have significantly reduced the number of cases of septicaemia. This success has constituted additional retrospective evidence for the water-borne origin of these infections.

Type
Research Article
Information
Epidemiology & Infection , Volume 98 , Issue 1 , February 1987 , pp. 5 - 14
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Adams, D., Atkinson, H. M. & Woods, W. H. (1983). Aeromonas hydrophila typing scheme based on patterns of agglutination with erythrocytes and yeast cells. Journal of Clinical Microbiology 17, 422–427.Google Scholar
Burke, V., Robinson, J., Cooper, M., Beaman, J., Partridge, K., Peterson, D. & Gracey, M. (1984 a). Biotyping and virulence factors in clinical and environmental isolates of Aeromonas species. Applied and Environmental Microbiology 47, 1146–1149.CrossRefGoogle ScholarPubMed
Burke, V., Robinson, J., Gracey, M., Peterson, D. & Partridge, K. (1984 b). Isolation of Aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates. Applied and Environmental Microbiology 48, 361–366.CrossRefGoogle ScholarPubMed
Conn, H. O. (1964). Spontaneous peritonitis and bacteremia in Laennec's cirrhosis caused by enteric organisms. Annals of Internal Medicine 60, 568–580.CrossRefGoogle ScholarPubMed
Cookson, B. D., Houang, E. C. & Lee, J. V. (1984). The use of biotyping system to investigate an unusual clustering of bacteraemias caused by Aeromonas species. Journal of Hospital Infection 5, 205–209.CrossRefGoogle ScholarPubMed
Goullet, Ph. & Picard, B. (1984). Typage électrophorétique des estérases d'Escherichia coli au cours de septicémies. La Presse Médicale 13. 1079–1081.Google Scholar
Hanson, P. G., Standridge, J., Jarrett, F. & Maki, D. G. (1977). Freshwater wound infection due to Aeromonas hydrophila. Journal of the American Medical Association 238, 1053–1054.CrossRefGoogle ScholarPubMed
Hazen, T. C., Fliermans, C. B., Hirsch, R. P. & Esch, G. W. (1978). Prevalence and distribution of Aeromonas hydrophila in the United States. Applied and Environmental Microbiology 36, 731–738.Google Scholar
Janda, J. M., Bottone, E. J., Skinner, C. V. & Calcaterra, D. (1983). Phenotypic markers associated with gastrointestinal Aeromonas hydrophila isolates from symptomatic children. Journal of Clinical Microbiology 17, 588–591.CrossRefGoogle ScholarPubMed
Janda, J. M., Reitano, M. & Bottone, E. J. (1984). Biotyping of Aeromonas isolates as a correlate to delineating a species-associated disease spectrum. Journal of Clinical Microbiology 19, 44–47.Google Scholar
Lawrence, S. H., Melnick, P. J. & Weimer, H. E. (1960). A comparison of serum proteins and enzymes by starch-gel electrophoresis. Proceedings of the Society for Experimental Biology and Medicine 105, 572–575.CrossRefGoogle ScholarPubMed
Le Chevallier, M. W., Evans, T. M., Seidler, R. J., Daily, O. P., Merrell, B. R., Rollins, D. M. & Joseph, S. W. (1982). Aeromonas sobria in chlorinated drinking water supplies. Microbial Ecology 8, 325–333.CrossRefGoogle Scholar
Lennox, E. S. (1955). Transduction of linked genetic characters of the host by bacteriophage Pl. Virology 1, 190–206.Google Scholar
McCracken, A. W. & Barkely, R. (1972). Isolation of Aeromonas species from clinical sources. Journal of Clinical Pathology 25, 970–975.Google Scholar
Mellersh, A. R., Norman, P. & Smith, G. H. (1984). Aeromonas hydrophila: an outbreak of hospital infection. Journal of Hospital Infection 5, 425–430.CrossRefGoogle ScholarPubMed
Picard, B., Arlet, G. & Goullet, Ph. (1984). Septicémies a Aeromonas hydrophila. Aspects épidémiologiques. Quinze observations. La Presse Medicale 13, 1203–1205.Google Scholar
Picard, B. & Goullet, Ph. (1984). Esterase electrophoresis: a new epidemiological screening test for Aeromonas hydrophila hospital infection. Journal of Hospital Infection 5, 335–337.CrossRefGoogle ScholarPubMed
Picard, B. & Goullet, Ph. (1985). Comparative electrophoretic profiles of esterases and of glutamate, lactate and malate dehydrogenases, from Aeromonas hydrophila, A. caviae and A. sobria. Journal of General Microbiology 131, 3385–3391.Google Scholar
Popoff, M. & Veron, M. (1976). A taxonomic study of the Aeromonas hydrophila–Aeromonas punctata group. Journal of General Microbiology 94, 11–22.Google Scholar
Popoff, M. Y., Coynault, C., Kiredjian, M. & Lemelin, M. (1981). Polynucleotide sequence relatedness among motile Aeromonas species. Current Microbiology 5, 109–114.CrossRefGoogle Scholar
Quinot, J. F., Delatte, P., Flye Sainte Marie, F. & Richard, C. (1982). Gangrène gazeuse à Aeromonas hydrophila. Un piège thérapeutique. La Presse Médicale 11, 2783–2784.Google Scholar
Sanyal, S. C., Singh, S. J. & Sen, P. C. (1975). Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides. Journal of Medical Microbiology 8, 195–198.Google Scholar
Uriel, J. (1961). Caractérisation des cholinestérases et d'autres estérases carboxyliques après électrophorèse et immuno-électrophorèse en gélose (application à l'étude des estérases du sérum humain normal). Annales de l'Institut Pasteur 101, 104–119.Google Scholar
Uriel, J. (1966). Méthode d'électrophorèse dans des gels d'acrylamide-agarose. Bulletin de la Société de Chimie Biologique 48, 969–982.Google Scholar
Wolff, R. L., Wiseman, S. L., Kitchens, C. S. (1980). Aeromonas hydrophila bacteremia in ambulatory immunocompromised hosts. American Journal of Medicine 68, 238–242.CrossRefGoogle ScholarPubMed