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Comparison of radioimmunoassay and the gel filtration technique for routine diagnosis of rubella during pregnancy

Published online by Cambridge University Press:  25 March 2010

H. O. Kangro ,
C. Jackson  and
R. B. Heath
H. O. Kangro
Affiliation:
Department of Virology, The London Hospital, London, E1
C. Jackson
Affiliation:
Department of Virology, The London Hospital, London, E1
R. B. Heath
Affiliation:
Department of Virology, The London Hospital, London, E1
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Summary

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Radioimmunoassay (RIA) for rubella-specific IgM antibodies was compared with haemagglutination-inhibition (HI) in conjunction with gel filtration for the diagnosis of rubella infection in pregnant women during a 1-year period. In total 476 women were investigated of whom 221 were tested for rubella IgM. Both techniques gave positive results with 64 sera, and RIA alone with one additional serum.

Difficulties associated with the removal of non-specific HI activity were encountered with four sera all of which were negative by RIA.

RIA was found to have practical advantages over the gel filtration method but is at present technically more difficult to perform.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 2 , October 1981 , pp. 249 - 255
Copyright
Copyright © Cambridge University Press 1981

References

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