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Antibody capture radioimmunoassay for anti-rubella IgM

Published online by Cambridge University Press:  25 March 2010

P. P. Mortimer ,
R. S. Tedder ,
M. H. Hambling ,
M. S. Shafi ,
F. Burkhardt  and
U. Schilt
P. P. Mortimer
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9 5HT
R. S. Tedder
Affiliation:
Department of Virology, Middlesex Hospital, Medical School, London W1N 8AA
M. H. Hambling
Affiliation:
Public Health Laboratory, Bridle Path, Leeds LS15 7TR
M. S. Shafi
Affiliation:
Public Health Laboratory, Central Middlesex Hospital, Park Royal, London NW10 7NS
F. Burkhardt
Affiliation:
Institut für Hygiene und Medizinische Microbiologie, Friedbühlstrasse 51, Bern, Switzerland
U. Schilt
Affiliation:
Institut für Hygiene und Medizinische Microbiologie, Friedbühlstrasse 51, Bern, Switzerland
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Summary

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An M-antibody capture radioimmunoassay (MACRIA) for anti-rubella IgM was developed. Under optimum conditions positive serum specimens bound up to 20 times as much radioactivity as negative specimens. Positive reactions were expressed in arbitrary units/ml by comparison with a calibration curve derived from results obtained with dilutions of a standard serum.

The specificity of the assay was confirmed by testing IgM and IgG rich fractions of positive sera. One hundred and forty specimens from blood donors, patients whose sera contained rheumatoid factor and patients with acute, non-rubella, virus infections were tested by MACRIA. No significant non-specific reactions were detected.

Paired sera from acute rubella (25 patients) and individual sera from suspected rubella (69 patients) were tested for anti-rubella IgM by MACRIA and by haemagglutination inhibition following sucrose-density-gradient fractionation. There was close agreement between the two methods. The capture assay was more sensitive and could be used to detect the weak IgM response in women given RA 27/3 vaccine. After the natural infection, the MACRIA was strongly positive for two months and remained weakly so for a further two months. Repeat testing of sera demonstrated good reproducibility of the assay.

MACRIA proved a simple, sensitive and specific test for anti-rubella IgM and compared favourably with currently used techniques.

Type
Research Article
Information
Journal of Hygiene , Volume 86 , Issue 2 , April 1981 , pp. 139 - 153
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

Bolton, A. E., & Hunter, W. M. (1973). The Labelling of Proteins to High Specific Radioactivities by Conjugation to a 125I-Containing Acylating Agent. Biochemical Journal 133, 529–39.CrossRefGoogle ScholarPubMed
Burkhardt, F., Schilt, U. & Saner, H. (1980). Virologische Diagnostik der Rötelninfektion, Befunde bei einer Rubella-Epidemie in einer Rekrutenschule. Schweizerische Medizinische Wochenschrift 110, 555–62.Google Scholar
Cradock-Watson, J. E., Ridehalgh, M. K. S., Pattison, J. R., Anderson, M. J. & Kangro, H. O. (1979). Comparison of immunofluorescence and radioimmunoassay for detecting IgM antibody in infants with the congenital rubella syndrome. Journal of Hygiene 83, 413–23.CrossRefGoogle ScholarPubMed
Diment, J. A. & Pepys, J. (1978). Immunosorbent Separation of IgG and IgM for the Radioimmunoassay of Specific Antibodies. Affinity Chromatography, (ed. Hoffmann-Ostenhof, O. et al. ), pp. 229–23. Oxford and New York: Pergamon Press.CrossRefGoogle Scholar
Duermeyer, W., Wielaard, F. & van der Veen, J. (1979). A New Principle for the Detection of Specific IgM Antibodies applied in an ELISA for Hepatitis A. Journal of Medical Virology 4, 25–32.CrossRefGoogle Scholar
Flehmig, B., Ranke, M., Berthold, H., & Gerth, H.J. (1979). A Solid-Phase Radioimmunoassay for Detection of IgM Antibodies to Hepatitis A Virus. Journal of Infectious Diseases 140, 169–75.CrossRefGoogle ScholarPubMed
Kurtz, J. B., Mortimer, P. P., Mortimer, P. R., Morgan-Capner, P., Shafi, M. S. & White, G. B. B. (1980). Rubella Antibody Measured by Radial Haemolysis. Characteristics and Performance of a Simple Screening Method for Use in Diagnostic Laboratories. Journal of Hygiene 84, 213–23.CrossRefGoogle ScholarPubMed
Mortimer, P. P., Parry, J. V. & Appleton, Hazel (1981). Diagnosis of recent hepatitis A infection: a comparison of two methods for detecting specific IgM. Journal of Hygiene (in the Press).CrossRefGoogle ScholarPubMed
Mortimer, P. P., Vandervelde, E. M., Parry, J. V., Cohen, B. J. & Tedder, R. S. (1981). The anti-HBc IgM Response in the Acute and Convalescent Phases of Acute Hepatitis. Journal of Infection (in the Press).CrossRefGoogle ScholarPubMed
Pattison, J. R. & Dane, D. S. (1975). The detection of specific IgM antibodies following infection with rubella virus. Journal of Clinical Pathology 28, 377–82.CrossRefGoogle ScholarPubMed
Public Health Laboratory Service Standing Advisory Committee on Viral Reagents (1978). Haemagglutination-inhibition test for the detection of rubella antibody. Journal of Hygiene, 81, 373–82.CrossRefGoogle Scholar
Roggendorf, M., Frösner, G. G., Deinhardt, F. & Scheid, R. (1980). Comparison of Solid Phase Test Systems for Demonstrating Antibodies Against Hepatitis A Virus (Anti-HAV) of the IgM-Class. Journal of Medical Virology 5, 47–62.CrossRefGoogle ScholarPubMed
Stoop, J. W., Zegers, B. J. M., Sander, P. C. & Ballieux, R. E. (1969). Serum Immunoglobulin Levels in Healthy Children and Adults. Clinical and Experimental Immunology 4, 101–12.Google ScholarPubMed
Tedder, R. S. & Wilson-Croome, Ruth (1981). Detection of IgM class antibody to hepatitis B core antigen: a comparison of two methods. Journal of Medical Virology, (in the Press).Google Scholar
Vesikari, T. & Vaheri, A. (1968). Rubella: a method for rapid diagnosis of recent infection by demonstration of the IgM antibody. British Medical Journal, i. 221–23.CrossRefGoogle Scholar