The following Memoir contains the results of researches made during the last three years. My investigations were originally directed to British lichens only, but they have subsequently and gradually embraced lichens from all parts of the world. The majority of Scotch species examined were collected by myself while on botanical tours in various parts of Scotland during the last ten years. Lowland species were collected chiefly in the counties of Perth, Edinburgh, and Dumfries; but also in Forfar, Fife, and others of the midland counties. In order to study alpine species I made a special tour among the highest of our Scotch mountains in the summer of 1856. I then visited the Braemar Highlands, Ben Lawers, Ben Nevis, and the Coollin Hills, in Skye. I have likewise studied the lichens of Don, now in the possession of Mr M'Nab, of the Ptoyal Botanic Garden, Edinburgh; the lichens collected by Maughan, M'Millan, and others, in the Herbarium of the Botanical Society of Edinburgh; those collected by the late Alexander Menzies, in the Menziesian Herbarium belonging to the Botanic Garden of Edinburgh; those collected by Borrer, Hooker, Carmichael, Gardiner, and others, in the magnificent Hookerian Herbarium at Kew; and I have also examined the valuable herbarium of the University of Edinburgh, under the care of Professor Balfour, and the herbarium of Dr Greville.