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Over-expression of GbRac1 gene in transgenic tobacco enhances disease resistance to Alternaria alternata in vitro

Published online by Cambridge University Press:  13 June 2008

Li Wei-Min ,
Wang Zhi-Xing  and
Jia Shi-Rong
Li Wei-Min
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wang Zhi-Xing
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jia Shi-Rong*
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
*Corresponding author. Email: [email protected]
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Abstract

GbRac1 gene was cloned from Gossypium barbadense with degenerate primers and 3′-RACE. Northern blot analysis indicated that GbRac1 mRNA was expressed abundantly in G. barbadense seedlings inoculated with Verticillium dehliae compared with mock-inoculated plants. A plant constitutive expression vector pRac harbouring GbRac1 gene was constructed and leaf discs of tobacco (Nicotiana tabacum L. cv. NC89) were transformed with pRac by Agrobacterium-mediated transformation. Disease challenge test of detached leaves of the transgenic plants by inoculation with Alternaria alternata showed that resistance was enhanced dramatically compared with the non-transgenic plants. Results suggest that GbRac1 gene might have potential application in the genetic engineering of plants with enhanced disease resistance.

Keywords

Gossypium barbadenseGTP-binding protein Ractransgenic tobaccoAlternaria alternatadisease resistance
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 73 - 77
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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