Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-28T11:42:05.541Z Has data issue: false hasContentIssue false
  • English
  • Français

AFLP analyses on genetic diversity and structure of Eupatorium adenophorum populations in China

Published online by Cambridge University Press:  27 June 2008

Huang Wen-Kun ,
Guo Jian-Ying ,
Wan Fang-Hao ,
Gao Bi-Da  and
Xie Bing-Yan
Huang Wen-Kun
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China
Guo Jian-Ying
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Wan Fang-Hao*
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Gao Bi-Da*
Affiliation:
College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China
Xie Bing-Yan
Affiliation:
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
*Corresponding author. E-mail: [email protected] or [email protected]
*Corresponding author. E-mail: [email protected] or [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Eupatorium adenophorum (crofton weed) is one of the most widespread invasive species in China. Its genetic diversity and population structure in China were analysed by amplified fragment length polymorphism (AFLP). Three primer pairs were selected for the analysis and 490 bands were produced from 62 E. adenophorum populations selected from five major geographic areas. A total of 328 of the bands showed polymorphism [percentage of polymorphic bands (PPB)=59.4%]. Diversity levels of populations were relatively high (mean expected heterozygosity=0.154, mean Shannon index=0.241). At the regional level, the AMOVA indicated that about 70.25% of variation in the data set was from genotypic variations within populations, whereas 8.04% of the variation was due to regional differences, and the remaining 21.71% to differences among populations within the provincial regions. Cluster analysis based on the unweighted pair-group method using the method of arithmetic averages (UPGMA) grouped the majority of E. adenophorum populations into four main clusters, which correspond to their geographic regions. It is concluded that E. adenophorum spread mainly by wind or water and its genetic diversity level in newly invaded areas was lower than that in formerly colonized areas.

Keywords

AFLPinvasive speciesEupatorium adenophorum (=Ageratina adenophora)genetic diversitygenetic variation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 33 - 41
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(6): 992–1000

References

Amsellem, L, Noyer, JL, Bourgeois, T and Hossaert-Mckey, M (2000) Comparison of genetic diversity of the invasive weed Rubus alceifolius Poir. (Rosaceae) in its native range and in areas of introduction, using amplified fragment length polymorphism (AFLP) markers. Molecular Ecology 9(18): 443–455.CrossRefGoogle ScholarPubMed
Carmen, P, Stephen, K and Fernando, G (1999) A population genetic study of the endangered plant species Limonium dufourii (Plumbaginaceae) based on amplified fragment length polymorphism (AFLP). Molecular Ecology 8: 645–657.Google Scholar
Doyle, JJ (1991) DNA protocols for plants – CTAB total DNA isolation. In: Hewitt, GM, Johnston, AWB and Young, JPW (editors) A Molecular Technique in Taxonomy. Berlin: Springer-Verlag, pp. 283–293.CrossRefGoogle Scholar
Genton, BJ, Shykoff, JA and Giraud, T (2005) High genetic diversity in French invasive populations of common ragweed, Ambrosia artemisiifolia, as a result of multiple sources of introduction. Molecular Ecology 14: 4275–4285.CrossRefGoogle ScholarPubMed
Huang, WK, Guo, JY, Wan, FH, Gao, BD and Xie, BY (2006) Genomic DNA extraction method and AFLP amplification reaction system of Ageratina adenophora Sprengel. Journal of Wuhan Botanical Research 24(6): 498–504 (in Chinese with English abstract).Google Scholar
Hill, M, Witsenboer, H, Zabeau, M, Vos, P, Kesseli, R and Michelmore, R (1996) PCR-based fingerprint using AFLP as a tool for studying genetic relationships in Lactuca sp. Theoretical and Applied Genetics 93: 1202–1210.CrossRefGoogle Scholar
Keiper, F and McConchie, R (2000) An analysis of genetic variation in natural populations of Sticherus flabellatus [R. Br. (St John)] using amplified fragment length polymorphism (AFLP) markers. Molecular Ecology 9: 571–581.CrossRefGoogle Scholar
Li, WY and Gu, WC (2003) AFLP analysis on genetic diversity of Quercus mongolica populations. Scientia Silvae Sinicae 39(5): 29–36 (in Chinese with English abstract).Google Scholar
Lu, P, Sang, WG and Ma, KP (2005) Progress and prospects in research: an exotic invasive species, Eupatorium adenophorum. Acta Phytoecologica Sinica 29(6): 1029–1037 (in Chinese with English abstract).Google Scholar
Lu, ZJ and Ma, KP (2004) The influence of topographical factors on the invasion of the alien species, Eupatorium adenophorum. Acta Phytoecologica Sinica 28(6): 761–767 (in Chinese with English abstract).Google Scholar
Mantel, NA (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27: 209–220.Google Scholar
Nesbitt, KA, Potts, BM, Vaillancourt, RE, West, AK and Reid, JB (1995) Partitioning and distribution of RAPD variation in a forest tree species, Eucalyptus globulus (Myrtaceae). Heredity 74: 628–637.CrossRefGoogle Scholar
O'Hanlon, PC, Peakall, R and Briese, DT (1999) Amplified fragment length polymorphism (AFLP) reveals introgression in weedy Onopordum thistles: hybridization and invasion. Molecular Ecology 8: 1239–1246.CrossRefGoogle ScholarPubMed
Portis, E, Barchi, L, Acquadro, A, Macua, JI and Lanteri, S (2005) Genetic diversity assessment in cultivated cardoon by AFLP (amplified fragment length polymorphism) and microsatellite markers. Plant Breeding 124: 299–304.CrossRefGoogle Scholar
Qiang, S (1998) The history and status of the study on crofton weed (Eupatorium adenophorum Spreng.) a worst worldwide weed. Journal of Wuhan Botany Research 16: 366–372 (in Chinese with English abstract).Google Scholar
Quagliaro, G, Vischi, M, Tyrka, M and Olivieri, AM (2001) Identification of wild and cultivated sunflower for breeding purposes by AFLP markers. Journal of Heredity 92: 38–42.CrossRefGoogle ScholarPubMed
Raccuia, SA, Mainolfi, A, Mandolino, AG and Melilli, MG (2004) Genetic diversity in Cynara cardunculus revealed by AFLP markers: comparison between cultivars and wild types from Sicily. Plant Breeding 123: 280–284.CrossRefGoogle Scholar
Rohlf, FJ (2002) NTSYSpc: Numerical Taxonomy System, ver. 2.1. Setauket, New York: Exeter Publishing.Google Scholar
Schneider, S, Kueffer, JM, Roessli, D and Excoffier, L (1997) ARLEQUIN, Version 1.1: Software for Population Genetic Data Analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland.Google Scholar
Sokal, RR, Jacquez, GM and Wooten, M (1989) Spatial autocorrelation analysis of migration and selection. Genetics 121: 845–855.CrossRefGoogle ScholarPubMed
Vos, P, Hogers, R, Bleeker, M, et al. (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23(21): 4407–4414.CrossRefGoogle ScholarPubMed
Wang, R and Wang, YZ (2006) Invasion dynamics and potential spread of the invasive alien plant species Eupatorium adenophorum (Asteraceae) in China. Diversity and Distributions 12: 397–408.CrossRefGoogle Scholar
White, G and Powell, W (1997) Isolation and characterization of microsatellite loci in Swieteniea humilis (Meliacae): An endangered tropical hardwood species. Molecular Ecology 4: 851–860.CrossRefGoogle Scholar
Yeh, FC, Yang, R and Boyle, T (1999) Popgene, version 1.32. Microsoft window-based freeware for population genetic analysis. University of Alberta, Edmonton. Available at: http://www.ualberta.ca/fyeh/index.htm.Google Scholar
Zhao, GJ and Ma, YP (1989) Investigation and study on the distribution and damage of Eupatorium adenophorum in Yunnan Province. Journal of Weed Science 3: 37–40 (in Chinese with English abstract).Google Scholar