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Expression of modified VHb gene and fused insectidal gene in tobacco

Published online by Cambridge University Press:  27 June 2008

Wang You-Ru ,
Cui Hong-Zhi  and
Guo San-Dui
Wang You-Ru*
Affiliation:
Hubei Normal University, Huangshi 435002, China Biotechnology Research Institute of Chinese Academy of Agricultural Science, Beijing 100081, China
Cui Hong-Zhi
Affiliation:
Biotechnology Research Institute of Chinese Academy of Agricultural Science, Beijing 100081, China
Guo San-Dui
Affiliation:
Biotechnology Research Institute of Chinese Academy of Agricultural Science, Beijing 100081, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

The highly efficient plant binary expression vector pGBIF4ABCVHB was constructed using the artificially modified, synthesized VHb gene and fused insectidal gene (GFMcryIA and CPTI). Transgenic tobacco plants were obtained by Agrobacterium-mediated transformation. For 32 plants, the bivalent genes were inserted successfully into the tobacco genome and detected by polymerase chain reaction (PCR) amplification. Southern blot and Western blot analyses showed that VHb gene was expressed in the transgenic plants. Toxicity assay indicated that the fused insectidal gene expressed pesticidal toxin protein. The net weight of transgenic tobacco plants exceeded that of non-transgenic ones by 8%. Compared to non-transgenic tobacco plants, transgenic plants appeared to be high-yielding, insect-resistant varieties.

Keywords

VHb genefused insectidal genetransgenic plantpest resistanceyield
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 27 - 31
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(6): 982–986

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