Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-24T02:25:27.919Z Has data issue: false hasContentIssue false
  • English
  • Français

EGFP expression in goat mammary epithelial cells

Published online by Cambridge University Press:  12 February 2007

Zheng Yue-Mao ,
An Zhi-Xing ,
Peng Xin-Rong ,
Shi Yu-Qiang  and
Zhang Yong
Zheng Yue-Mao
Affiliation:
Institute of Bio-engineering, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
An Zhi-Xing
Affiliation:
Institute of Bio-engineering, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Peng Xin-Rong
Affiliation:
Institute of Bio-engineering, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Shi Yu-Qiang
Affiliation:
Institute of Bio-engineering, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Zhang Yong*
Affiliation:
Institute of Bio-engineering, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
*
*Corresponding author: E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Primary culture of goat mammary gland cells was achieved by outgrowth of migrating cells from fragments of tissue. The fibroblast and epithelial cells were purified according to their different sensitivity to trypsin and the characteristics of goat mammary epithelial cells were observed under a light microscope. The results showed that the purified goat mammary epithelial cells retained normal characteristics until the 15th passage. The purified mammary epithelial cells propagated and formed a dome-like structure that resembled a nipple and was called a ‘milk orb’. The mammary epithelial cells could produce and secrete milk. There were different cell types: the majority were short shuttle-like or polygons, resembling a beehive; while some were large, flat and round; and others were elongated. The enhanced green fluorescent protein (EGFP) gene was transferred successfully into the goat mammary epithelial cells using electrotransfection, and its expression was observed under a fluoroscope.

Keywords

EGFP genegoatmammary epithelial cells
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 1 , April 2006 , pp. 71 - 74
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Buehring, GC (1972) Culture of human mammary epithelial cells: keeping abreast with a new method. National Cancer Institute 49(5): 1433–1434.Google ScholarPubMed
Huynh, HT and Pollak, M (1995) HH2a, an immortalized bovine mammary epithelial cell line expresses the gene encoding mammary derived growth inhibitor (MDGI). In Vitro Cell Developmental Biology Animal 31A 25–29.CrossRefGoogle Scholar
Li, XC, Ren, FL and Zhang, Y (2005) Establishment and ultrastructural characterization of goat mammary epithelial cell line. Acta Veterinary et Zootechnica Sinica 36(2): 121–126.Google Scholar
Mselli-Lakhal, L, Guiguen, F and Fornazero, C (2001) Immortalized goat milk epithelial cell lines replicate CAEV at high level. Veterinary Research 32(5): 429–440.Google Scholar
Ouyang, WQ and Qian, JF (1999) Establishment of goat mammary epithelial cell line. Chinese Journal of Veterinary Science 19(6): 561–562.Google Scholar
Pantschenko, AG, Woodcock-Mitchell, J, Bushmich, SL and Yang, TJ (2000) Establishment and characterization of a caprine mammary epithelial cell line (CMEC). In Vitro Cell Developmental Biology Animal 36(1): 26–37.Google Scholar
Pickett, PB, Pitelka, DR and Hamamoto, ST (1975) Occluding junctions and cell behavior in primary cultures of normal and neoplastic mammary gland cells. Cell Biology 66(2): 316–332.Google Scholar
Stampfer, M, Hallowes, RC and Hackett, AJ (1980) Growth of normal human mammary cells in culture. In Vitro Cell Developmental Biology Animal 16(5): 415–425.Google Scholar
Van den Hoff, MJB, Christoffels, VM, Labruyere, WT et al. , (1997) Electrotransfection with intracellular buffer. In: Nickoloff, JA (editor) Methods in Molecular Biology. Totowa: Humana Press, p. 185.Google Scholar
Warburtorr, MJ, Head, LP and Rudland, PS (1981) Redistribution of fibronectin and cytoskeletal proteins during the differentiation of rat mammary tumor cells in vitro. Experimental Cell Research 132(1): 57–66.Google Scholar
Zavition, B, Gorewit, RC and Politis, I (1995) Subcloning the MAT-T bovine mammary epithelial cell line: morphology, growth, properties, and cytogenetic analysis of clonal cells. Journal of Dairy Science 78: 515–527.CrossRefGoogle Scholar
Zavition, B, van Duffelen, M and Schaeffer, W (1996) Establishment and characterization of a bovine mammary epithelial cell line with unique properties. In Vitro Cell Developmental Biology Animal 32(3): 138–148.CrossRefGoogle Scholar