Twenty Nearctic species of Myllaena are recognized. All are redescribed or newly described and illustrated. A key for their identification, and maps of their distribution are provided. The species are arranged into four groups: the Cuneata, Insomnis, Potawatomi, and Seminole. Seven species are described as new: hopi (Arizona, Mexico), hopkinton (Massachusetts), kaskaskia (eastern U.S.A.), magnolia (Florida), potawatomi (transcontinental, also in Haiti and Jamaica), serrano (California, Baja California of Mexico), and seminole (southern U.S.A. and Illinois). Newly established synonymies are (the second name of each pair being valid): abdita Casey, 1911 = audax Casey, 1911; brevivestis Casey, 1911 = ludificans Casey, 1911; dissimulons Casey, 1911 = audax Casey, 1911; immunda Casey, 1911 = arcana Casey, 1911; molesta Casey, 1911 = audax Casey, 1911; obscurata Casey, 1911 = insipiens Casey, 1911; scobinella Casey, 1911 = decréta Casey, 1911; umbra Casey, 1911 = decreta Casey, 1911; and vegeta Casey, 1911 = audax Casey, 1911.
Fourteen lectotypes are designated. A checklist of Nearctic species is added. The systematics, phylogeny, and zoogeography of the genus and' species are discussed.