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ELECTROPHORETIC STUDY OF ENZYMES FROM A GLOSSINA FUSCIPES FUSCIPES NEWSTEAD POPULATION FROM WESTERN KENYA

Published online by Cambridge University Press:  31 May 2012

G.F. Rajendram
G.F. Rajendram
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi, Kenya
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Abstract

Enzymes were investigated, by electrophoresis, in a population of Glossina fuscipes fuscipes Newstead collected from Rusinga Island in Lake Victoria, Western Kenya.

The following enzymes were tested: glucose phosphate isomerase, glucose-6-phosphate dehydrogenase (G6PDH), hexokinase. isocitrate dehydrogenase (IDH), malate-dehydrogenase (MDH), phosphoglucomutase, and xanthine dehydrogenase (XDH).

Single monomorphic bands were stained by the following enzymes apparently under the control of single loci: G6PDH, MDH, and XDH. The enzyme IDH showed two bands with very close mobilities and no variation among individuals in the population. Hence IDH was considered as representing a single locus. Glucose phosphate isomerase manifested three alleles and apparently six genotypes. Phosphoglucomutase manifested a double-banded pattern representing an autosomal locus.

Résumé

Les enzymes d’une population de Glossina fuscipes fuscipes Newstead, provenant de l’Île Rusinga du Lac Victoria, de l’ouest du Kenya, ont été étudiés en utilisant l’électrophorèse.

Les enzymes suivantes ont été mise à l’épreuve : l’isomérase du glucose phosphate, le glucose-6-phosphate déshydrogénase (G6PDH), l’hexokinase, le déshydrogénase d’isocitrate (DHI), le malate-déshydrogénase (MDH), la phosphoglucomutase et le déshydrogénase de xanthine (DHX).

Les bandes uniques monomorphiques, qui sont apparemment sous le contrôle d’un seul locus, ont été teintes par les enzymes suivantes : G6PDH, MDH et DHX. L’enzyme DHI a démontré deux bandes avec les mobilités très rapprochées et n’ayant aucune variation parmi les individus de la population. Ainsi, DHI a été considéré pour représenter un seul locus. L’isomérase du glucose phosphate a signalé trois allèles et probablement six génotypes. La phosphoglucomutase a démontré un patron de bandes doubles qui représente un locus autosomique.

Type
Articles
Information
The Canadian Entomologist , Volume 123 , Issue 2 , April 1991 , pp. 295 - 298
Copyright
Copyright © Entomological Society of Canada 1991

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