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A molecular approach to identify prey of the southern rock lobster

Published online by Cambridge University Press:  28 April 2008

K.S. Redd ,
S.N. Jarman ,
S.D. Frusher  and
C.R. Johnson
K.S. Redd*
Affiliation:
School of Zoology, University of Tasmania, Private Bag 5, Hobart, TAS 7001, Australia Tasmanian Aquaculture and Fisheries Institute, Marine Research Laboratories, University of Tasmania, Private Bag 49, Hobart, TAS 7001, Australia
S.N. Jarman
Affiliation:
Department of the Environment and Heritage, Australian Antarctic Division, 203 Channel Highway, Kingston, TAS 7050, Australia
S.D. Frusher
Affiliation:
Tasmanian Aquaculture and Fisheries Institute, Marine Research Laboratories, University of Tasmania, Private Bag 49, Hobart, TAS 7001, Australia
C.R. Johnson
Affiliation:
School of Zoology, University of Tasmania, Private Bag 5, Hobart, TAS 7001, Australia
*
*Author for correspondence Fax: (+61) 3 6226 2745 E-mail: [email protected]
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Abstract

We demonstrate the use of molecular techniques to detect specific prey consumed by the southern rock lobster (Jasus edwardsii). A quick and non-lethal method was used to collect rock lobster faecal material and a molecular protocol was employed to isolate prey DNA from faecal samples. The isolated DNA was amplified using the polymerase chain reaction (PCR) with PCR primers designed to target specific prey items. Feeding experiments determined that DNA from black-lipped abalone (Haliotis rubra) and sea urchins (Centrostephanus rodgersii and Heliocidaris erythrogramma) can be detected in rock lobster faecal samples within seven hours and remains present for up to 60 h after ingestion.

Keywords

Jasus edwardsiiHaliotis rubrapolymerase chain reaction (PCR)prey detectionrock lobster diet
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 98 , Issue 3 , June 2008 , pp. 233 - 238
Copyright
Copyright © 2008 Cambridge University Press

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