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Taxonomic identification of Macrolophus pygmaeus and Macrolophus melanotoma based on morphometry and molecular markers

Published online by Cambridge University Press:  24 September 2012

C. Castañé ,
N. Agustí ,
J. Arnó ,
R. Gabarra ,
J. Riudavets ,
J. Comas  and
Ó. Alomar
C. Castañé*
Affiliation:
IRTA, Entomology, Carretera de Cabrils Km 2, 08348-Cabrils, Spain
N. Agustí
Affiliation:
IRTA, Entomology, Carretera de Cabrils Km 2, 08348-Cabrils, Spain
J. Arnó
Affiliation:
IRTA, Entomology, Carretera de Cabrils Km 2, 08348-Cabrils, Spain
R. Gabarra
Affiliation:
IRTA, Entomology, Carretera de Cabrils Km 2, 08348-Cabrils, Spain
J. Riudavets
Affiliation:
IRTA, Entomology, Carretera de Cabrils Km 2, 08348-Cabrils, Spain
J. Comas
Affiliation:
Universitat Politècnica de Catalunya, Departament d'Enginyeria Agroalimentària i Biotecnologia, C/Esteve Terrades 8, 08860-Castelldefels, Spain
Ó. Alomar
Affiliation:
IRTA, Entomology, Carretera de Cabrils Km 2, 08348-Cabrils, Spain
*
*Author for correspondence Fax: +0034937533954 E-mail: [email protected]
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Abstract

Two Macrolophus species, M. melanotoma (=M. caliginosus) and M. pygmaeus, have been referred to as efficient predators of several key pests on vegetable crops in Europe. However, due to the great morphological similarity of these species, they have been confused, with important consequences for inoculative releases of these predators in greenhouses and for the conservation of their natural populations on greenhouse and outdoor crops. In this work, we developed tools to identify these morphologically very similar species. We first confirmed the specific status of two Macrolophus populations collected on their respective host plants (Dittrichia viscosa and tomato) through crossing experiments. Then, using multivariate morphometric analysis, we proposed a linear discriminant function that combines head measurements separating males from the two species without error. Finally, we designed specific primers for a mitochondrial DNA region that were able to distinguish field-collected Macrolophus individuals through conventional PCR. In conclusion, the tools developed in the present study will allow reliable identification of the Macrolophus species present in crops and in the native flora that are the source of populations that colonise them. They will also allow correct identification of mass reared Macrolophus to be introduced in greenhouse crops in inoculative releases.

Keywords

Macrolophus caliginosusM. melanotomaM. pygmaeustaxonomic characterisationmultivariate morphometryspecific primerscryptic species
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 2 , April 2013 , pp. 204 - 215
Copyright
Copyright © Cambridge University Press 2012

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