Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-28T01:15:37.307Z Has data issue: false hasContentIssue false
  • English
  • Français

Rapid diagnosis of the economically important fruit fly, Bactrocera correcta (Diptera: Tephritidae) based on a species-specific barcoding cytochrome oxidase I marker

Published online by Cambridge University Press:  05 March 2013

F. Jiang ,
Z.H. Li ,
Y.L. Deng ,
J.J. Wu ,
R.S. Liu  and
N. Buahom
F. Jiang
Affiliation:
Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Haidian District, Beijing 100193, P.R. China
Z.H. Li*
Affiliation:
Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Haidian District, Beijing 100193, P.R. China
Y.L. Deng
Affiliation:
Xishuangbanna Entry-Exit Inspection and Quarantine Bureau, Xishuangbanna, Yunnan 666100, P.R. China
J.J. Wu
Affiliation:
Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, P.R. China
R.S. Liu
Affiliation:
Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Haidian District, Beijing 100193, P.R. China Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, P.R. China
N. Buahom
Affiliation:
Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Haidian District, Beijing 100193, P.R. China
*
* Author for correspondence Phone: +86-10-62731299 Fax: +86-10-62733404 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The guava fruit fly, Bactrocera correcta (Bezzi) (Diptera: Tephritidae), is an invasive pest of fruit and vegetable crops that primarily inhabits Southeast Asia and which has the potential to become a major threat within both the Oriental and Australian oceanic regions as well as California and Florida. In light of the threat posed, it is important to develop a rapid, accurate and reliable method to identify B. correcta in quarantine work in order to provide an early warning to prevent its widespread invasion. In the present study, we describe a species-specific polymerase chain reaction assay for the diagnosis of B. correcta using mitochondrial DNA cytochrome oxidase I (mtDNA COI) barcoding genes. A B. correcta-specific primer pair was designed according to variations in the mtDNA COI barcode sequences among 14 fruit fly species. The specificity and sensitivity of the B. correcta-specific primer pair was tested based on the presence or absence of a band in the gel profile. A pair of species-specific B. correcta primers was successfully designed and named BCOR-F/BCOR-R. An ∼280 bp fragment was amplified from specimens belonging to 17 geographical populations and four life stages of B. correcta, while no such diagnostic bands were present in any of the 14 other related fruit fly species examined. Sensitivity test results demonstrated that successful amplification can be obtained with as little as 1 ng μl−1 of template DNA. The species-specific PCR analysis was able to successfully diagnose B. correcta, even in immature life stages, and from adult body parts. This method proved to be a robust single-step molecular technique for the diagnosis of B. correcta with respect to potential plant quarantine.

Keywords

Bactrocera correctamolecular diagnosisspecies-specific PCRmtDNA COIDNA barcode
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 3 , June 2013 , pp. 363 - 371
Copyright
Copyright © Cambridge University Press 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allwood, A.J. & Drew, R.A.I. (1996) Management of fruit flies in the Pacific. A regional symposium, Nadi, Fiji 28–31 October 1996, 23.Google Scholar
Anderson, P.J. & Dixon, W.N. (2008) Triology 47, No. 1, 7. Available online at http://www.freshfromflorida.com/pi/enpp/triology/archive/4701.pdf (accessed 2 September 2011).Google Scholar
Armstrong, K.F., Cameron, C.M. & Frampton, E.R. (1997) Fruit fly (Diptera: Tephritidae) species identification: a rapid diagnostic technique for quarantine application. Bulletin of Entomological Research 87, 111–118.Google Scholar
Asokan, R., Krishna Kumar, N.K. & Abraham, V. (2007) Molecular identification of fruit flies, Bactrocera spp. (Diptera: Tephritidae) using mitochondrial cytochrome oxidase I. Current Science 93, 1668–1669.Google Scholar
Barcenas, N.M., Unruh, T.R. & Neven, L.G. (2005) DNA diagnostics to identify internal feeders (Lepidoptera: Tortricidae) of pome fruits of quarantine importance. Journal of Economic Entomology 98, 299–306.Google Scholar
Chua, T.H., Song, B.K. & Chong, Y.V. (2010) Development of allele-specific single-nucleotide polymorphism-based polymerase chain reaction markers in cytochrome oxidase I for the differentiation of Bactrocera papaya and Bactrocera carambolae (Diptera: Tephritidae). Journal of Economic Entomology 103, 1994–1999.Google Scholar
CPC, CABI. (2012) Crop Protection Compendium. Available online at http://www.cabi.org/cpc/.Google Scholar
Drew, R.A.I. & Raghu, S. (2002) The fruit fly fauna (Diptera: Tephritidae: Dacinae) of the rainforest habitat of the Western Ghats, India. Raffles Bulletin of Zoology 50, 327–352.Google Scholar
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.Google ScholarPubMed
Hebert, P.D.N., Cywinska, A., Ball, S.L. & DeWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings the Royal Society B 270, 313–321.CrossRefGoogle ScholarPubMed
Hosseini, R., Keller, M.A., Schmidt, O. & Framenau, V.W. (2007) Molecular identification of wolf spiders (Araneae: Lycosidae) by multiplex polymerase chain reaction. Biological Control 40, 128–135.CrossRefGoogle Scholar
Liang, G.X., Yang, G.H., Liang, F., Si Tu, B.L. & Liang, X.D. (1996) The Species of Dacini (Diptera: Tephritidae) of Asian-Pacific region. Guangzhou, China, Guangdong Science and Technology Press.Google Scholar
Liu, L.J., Liu, J.Q., Wang, Q.L., Ndayiragije, P., Ntahimpera, A., Nkubaye, E., Yang, Q.Q. & Li, Z.H. (2011) Identification of Bactrocera invadens (Diptera: Tephritidae) from Burundi, based on morphological characteristics and DNA barcode. African Journal of Biotechnology 10, 13623–13630.Google Scholar
Muraji, M. & Nakahara, S. (2001) Phylogenetic relationships among fruit flies, Bactrocera (Diptera, Tephritidae), based on the mitochondrial rDNA sequences. Insect Molecular Biology 10, 549–559.Google Scholar
Tan, K.H., Tokushima, I., Ono, H. & Nishida, R. (2011) Comparison of phenylpropanoid volatiles in male rectal pheromone gland after methyl eugenol consumption, and molecular phylogenetic relationship of four global pest fruit fly species: Bactrocera invadens, B. dorsalis, B. correcta and B. zonata . Chemoecology 21, 25–33.Google Scholar
Vijaysegaran, S. (1996) Fruit fly research and development in tropical Asia. pp. 21–29 in Management of Fruit Flies in the Pacific–A Regional Symposium.Google Scholar
Wang, W.X., Yu, F., Zhang, Z. & Lin, X.H. (2010) Advances in rapid identification methods for the quarantined fruit flies. Plant Protection 36, 39–43.Google Scholar
Wang, X.J. (1996) The fruit flies (Diptera: Tephritidae) of the East Asian Region. Acta Zootaxonomica Sinica 21, 1–338.Google Scholar
Wang, X.J. & Zhao, M.Z. (1989) Species of genus Dacus Fabricius (Diptera: Tephritidae) from China. Acta Zootaxonomica Sinica 14, 209–219.Google Scholar
Weems, H.W. & Fasulo, T.R. (2011) Guava Fruit Fly, Bactrocera correcta (Bezzi) (Insecta: Diptera: Tephritidae). University of Florida IFAS Extension, EENY200/IN357.Google Scholar
White, I.M. & Elson-Harris, M.M. (1992) Fruit Flies of Economic Significance: Their Identification and Bionomics. Wallingford, Oxon, UK, CAB International.Google Scholar
Wu, J.J., Hu, X.N., Zhao, J.P., Liang, F. & Liang, G.Q. (2005) Rapid identification among 9 species of quarantine fruit flies (Diptera: Tephritidae) by PCR-RFLP. Plant Quarantine 19, 2–6.Google Scholar
Yang, Q.Q., Kucerova, Z., Li, Z.H., Kalinovic, I., Stejskal, V., Opit, G. & Cao, Y. (2012) Diagnosis of Liposcelis entomophila (Insecta: Psocodea: Liposcelididae) based on morphological characteristics and DNA barcodes. Journal of Stored Products Research 48, 120–125.Google Scholar
Yu, D.J., Deng, Z.P., Chen, Z.L., Jiao, Y., Zhang, G.M., Kang, L., Yang, W.D. & Jin, X.Z. (2004 a) Method of the polymerase chain reaction for quarantine and identification of Bactrocera correcta . Plant Quarantine 18, 73–76.Google Scholar
Yu, D.J., Zhang, G.M., Chen, Z.L., Zhang, R.J. & Yin, W.Y. (2004 b) Rapid identification of Bactrocera latifrons (Dipt., Tephritidae) by real-time PCR using SYBR Green chemistry. Journal of Applied Entomology 128, 670–676.Google Scholar
Yu, D.J., Chen, Z.L., Zhang, R.J. & Yin, W.Y. (2005) Real-time qualitative PCR for the inspection and identification of Bactrocera philippinensis and Bactrocera occipitalis (Diptera: Tephritidae) using SYBR Green assay. The Raffles Bulletin of Zoology 53, 73–78.Google Scholar
Zhang, G.F., Meng, X.Q., Min, L., Qiao, W.N. & Wan, F.H. (2012) Rapid diagnosis of the invasive species, Frankliniella occidentalis (Pergande): a species-specific COI marker. Journal of Applied Entomology 136, 410–420.Google Scholar
Zhang, L. & Zhang, Z.Y. (2007) Random amplified polymorphic DNA identification of six Bactrocera (Diptera: Tephritidae) species in Yunnan Province of Southwest China. Chinese Journal of Applied Ecology 18, 1163–1166.Google Scholar
Zhao, J.P., Liang, F., Liang, G.Q., Hu, X.N., Ma, J. & Lin, L. (2007) Morphological identification of Bactrocera correcta and Bactrocera zonata . Chinese Bulletin of Entomology 44, 904–905.Google Scholar