Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-28T01:06:34.588Z Has data issue: false hasContentIssue false
  • English
  • Français

Clonal turnover of MACE-carrying peach-potato aphids (Myzus persicae (Sulzer), Homoptera: Aphididae) colonizing Scotland

Published online by Cambridge University Press:  13 December 2007

L. Kasprowicz ,
G. Malloch ,
S. Foster ,
J. Pickup ,
J. Zhan  and
B. Fenton
L. Kasprowicz
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA
G. Malloch
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA
S. Foster
Affiliation:
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ
J. Pickup
Affiliation:
Scottish Agricultural Science Agency, 1 Roddinglaw Road, EdinburghEH12 9FJ
J. Zhan
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA
B. Fenton*
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA
*
*Author for correspondence Fax: +44 1382 562426 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Peach-potato aphids, Myzus persicae (Sulzer), collected in Scotland in the years 1995 and 2002–2004 were characterized using four microsatellite loci and three insecticide resistance mechanisms. From 868 samples, 14 multilocus genotypes were defined (designated clones A–N). Five of these (denoted A, B, H, M and N) carried modified acetylcholinesterase (MACE) resistance, the most recent resistance mechanism to have evolved in M. persicae. The current paper shows that the continued presence of MACE aphids is due to turnover, as clones A and B were replaced in field samples by clones H, M and N in later seasons. Thus, insecticide-resistant populations in Scotland can be attributed to multiple waves of rapid clone colonisations and not to the continued presence of stable resistant clones or mutation or sexual recombination in local populations. The MACE clones carried varying levels of the other insecticide resistance mechanisms, kdr and esterase. The presence of these mechanisms could alter the clones success in the field depending on insecticide spraying (positive selection) and resistance fitness costs (negative selection).

Keywords

Myzus persicaeinsecticide resistanceclonesmicrosatellite markers
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 98 , Issue 2 , April 2008 , pp. 115 - 124
Copyright
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anstead, J.A., Williamson, M.S., Eleftherianos, I. & Denholm, I. (2004) High-throughput detection of knockdown resistance in Myzus persicae using allelic discriminating quantitative PCR. Insect Biochemistry and Molecular Biology 34, 871–877.Google Scholar
Chia, W., Savakis, C., Karp, R., Pelham, H. & Ashburner, M. (1985) Mutation of the Adh gene of Drosophila melanogaster containing an internal tandem duplication. Journal of Molecular Biology 186, 679–688.CrossRefGoogle ScholarPubMed
Felsenstein, J. (1993) phylip (Phylogeny Information Package), Version 3.5c. University of Washington, Seattle.Google Scholar
Fenton, B., Woodford, J.A.T. & Malloch, G. (1998) Analysis of clonal diversity of the peach-potato aphid, Myzus persicae (Sulzer), in Scotland, UK and evidence for the existence of a predominant clone. Molecular Ecology 7, 1475–1487.Google Scholar
Fenton, B., Malloch, G., Navajas, M., Hillier, J. & Birch, A.N.E. (2003) Clonal composition of the peach-potato aphid Myzus persicae (Homoptera: Aphididae) in France and Scotland: Comparative analysis with IGS fingerprinting and microsatellite markers. Annals of Applied Biology 142, 255–267.CrossRefGoogle Scholar
Fenton, B., Malloch, G., Woodford, J.A.T., Foster, S.P., Anstead, J., Denholm, I., King, L. & Pickup, J. (2005) The attack of the clones: tracking the movement of insecticide-resistant peach-potato aphids Myzus persicae (Hemiptera: Aphididae). Bulletin of Entomological Research 95, 483–494.Google Scholar
Field, L.M., Devonshire, A.L., Ffrench-Constant, R.H. & Forde, B.G. (1989) Changes in DNA methylation are associated with loss of insecticide resistance in the peach-potato aphids Myzus persicae (Sulz). FEBS Letters 243, 323–327.Google Scholar
Foster, S.P., Harrington, R., Devonshire, A.L., Denholm, I., Devine, G.J. & Kenward, M.G. (1996) Comparative survival of insecticide-susceptible and resistant peach-potato aphids, Myzus persicae (Sulzer) (Hemiptera: Aphididae), in low temperature field trials. Bulletin of Entomological Research 86, 17–27.CrossRefGoogle Scholar
Foster, S.P., Denholm, I., Harling, Z.K., Moores, G.D. & Devonshire, A.L. (1998) Intensification of insecticide resistance in UK field populations of the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae) in 1996. Bulletin of Entomological Research 88, 127–130.Google Scholar
Foster, S.P., Denholm, I. & Devonshire, A.L. (2000) The ups and downs of insecticide resistance in peach-potato aphids (Myzus persicae) in the UK. Crop Protection 19, 873–879.Google Scholar
Foster, S.P., Harrington, R., Dewar, A.M., Denholm, I. & Devonshire, A.L. (2002) Temporal and spatial dynamics of insecticide resistance in Myzus persicae (Hemiptera: Aphididae). Pest Management Science 58, 895–907.CrossRefGoogle ScholarPubMed
Foster, S.P., Kift, N.B., Baverstock, J., Sime, S., Reynolds, K., Jones, J.E., Thompson, R. & Tatchell, G.M. (2003) Association of MACE-based insecticide resistance in Myzus persicae with reproductive rate, response to alarm pheromone and vulnerability to attack by Aphidius colemani. Pest Management Science 59, 1169–1178.Google Scholar
Foster, S.P., Denholm, I., Thompson, R., Poppy, G.M. & Powell, W. (2005) Reduced response of insecticide-resistant aphids and attraction of parasitoids to aphid alarm pheromone; a potential fitness trade-off. Bulletin of Entomological Research 95, 37–46.Google Scholar
Guillemaud, T., Brun, A., Anthony, N., Sauge, M.-H., Boll, R., Delorme, R., Fournier, D., Lapchin, L. & Vanlerberghe-Masutti, F. (2003) Incidence of insecticide resistance alleles in sexually-reproducing populations of the peach-potato aphid Myzus persicae (Hemiptera: Aphididae) from southern France. Bulletin of Entomological Research 93, 289–297.CrossRefGoogle ScholarPubMed
Jacob, F.H. (1941) The over-wintering of Myzus persicae Sulz. on brassica in north Wales. Annals of Applied Biology 28, 119–124.Google Scholar
Kosman, E. & Leonard, K.J. (2005) Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploid species. Molecular Ecology 14, 415–424.CrossRefGoogle ScholarPubMed
Malloch, G., Fenton, B. & Butcher, R.D.J. (2000) Molecular evidence for multiple infections of a new subgroup of Wolbachia in the European raspberry beetle Byturus tomentosus. Molecular Ecology 9, 77–90.CrossRefGoogle ScholarPubMed
Malloch, G., Highet, F., Kasprowicz, L., Pickup, J., Neilson, R. & Fenton, B. (2006) Microsatellite marker analysis of peach–potato aphids (Myzus persicae, Homoptera: Aphididae) from Scottish suction traps. Bulletin of Entomological Research 96, 1–10.Google Scholar
Mazzoni, E. & Cravedi, P. (2002) Analysis of insecticide-resistant Myzus persicae (Sulzer) populations collected in Italian peach orchards. Pesticide Management Science 58, 976–980.CrossRefGoogle ScholarPubMed
Moores, G.D., Devine, G.J. & Devonshire, A.L. (1994a) Insecticide-insensitive acetylcholinesterase can enhance esterase-based resistance in Myzus persicae and Myzus nicotianae. Pesticide Biochemistry and Physiology 49, 114–120.Google Scholar
Moores, G.D., Devine, G.J. & Devonshire, A.L. (1994b) Insecticide resistance due to insensitive acetylcholinesterase in Myzus persicae and Myzus nicotianae. BCPC conference – Pest and Diseases 1, 413–418.Google Scholar
Nabeshima, T., Kozaki, T., Tomita, T. & Kono, Y. (2003) An amino acid substitution on the second acetylcholinesterase in the pirimicarb-resistant strains of the peach-potato aphid, Myzus persicae. Biochemical and Biophysical Research Communications 37, 15–22.CrossRefGoogle Scholar
Page, R.D. (1996) Treeview: an application to display phylogenetic trees on personal computers. Computer Applications in Biosciences 12, 357–358.Google Scholar
Pozarowska, B.J. (1987) Studies on low temperature survival, reproduction and development in Scottish clones of Myzus persicae (Sulzer) and Aulocorthum solani (Kaltenbach) (Hemiptera: Aphididae) susceptible and resistant to organophosphates. Bulletin of Entomological Research 77, 123–134.Google Scholar
Sloane, M.A., Sunnucks, P., Wilson, A.C.C. & Hales, D. (2001) Microsatellite isolation, linkage group identification and determination of recombination frequency in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Genetical Research Cambridge 77, 251–260.CrossRefGoogle ScholarPubMed
Sneath, P.A. & Sokal, R.R. (1973) Numerical Taxonomy. 513 pp. San Francisco, CA, USA, W.H. Freeman Co.Google Scholar
Stanton, J.M., McNicol, C.D. & Steele, V. (1998) Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on polymerase chain reaction. Australasian Plant Pathology 27, 112–115.CrossRefGoogle Scholar
Terradot, L, Simon, J.-C., Leterme, N., Bourdin, D., Wilson, A.C.C., Gauthier, J.-P. & Robert, Y. (1999) Molecular characterization of clones of the Myzus persicae complex (Hemiptera: Aphididae) differing in their ability to transmit the potato leafroll luteovirus (PLRV). Bulletin of Entomological Research 89, 355–363.Google Scholar
van Emden, H.F., Eastop, V.F., Hughes, R.D. & Way, M.J. (1969) The ecology of Myzus persicae. Annual Review of Entomology 14, 197–268.Google Scholar
van Toor, R.F., Stephen, P., Foster, S.P., Anstead, J.A., Mitchinson, S., Fenton, B. & Kasprowicz, L. (in press) High proportion of the peach potato aphid Myzus persicae (Hemiptera:Aphididae) on field crops of potatoes in New Zealand carry insecticide resistance mechanisms. Crop Protection, in press.Google Scholar
Vorburger, C., Lancaster, M. & Sunnucks, P. (2003) Environmentally related patterns of reproductive modes in the aphid Myzus persicae and the predominanace of two ‘superclones’ in Victoria, Australia. Molecular Ecology 12, 3493–3504.Google Scholar
Zamoum, T., Simon, J.-C., Crochard, D., Ballanger, Y., Lapchin, L., Vanlerberghe-Masutti, F. & Guillemaud, T. (2005) Does insecticide resistance alone account for the low genetic variability of asexually reproducing populations of the peach-potato aphid Myzus persicae? Heredity 94, 630–639.CrossRefGoogle ScholarPubMed