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Urinary excretion of purine derivatives in Bos indicus×Bos taurus crossbred cattle

Published online by Cambridge University Press:  08 March 2007

Álvaro Ojeda ,
Ornella de Parra ,
Joaquím Balcells  and
Álvaro Belenguer
Álvaro Ojeda
Affiliation:
Instituto de Producción Animal, Facultad de Agronomía, Universidad Central de Venezuela, Maracay Apdo. 4579, Venezuela
Ornella de Parra
Affiliation:
Instituto de Producción Animal, Facultad de Agronomía, Universidad Central de Venezuela, Maracay Apdo. 4579, Venezuela
Joaquím Balcells*
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177-50 013, Spain
Álvaro Belenguer
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177-50 013, Spain
*
*Corresponding author: Dr Joaquím Balcells, fax +34 976 761590, email [email protected]
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Abstract

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Four experiments were performed to study the kinetics of purine metabolism and urinary excretion in Zebu crossbred cattle. Fasting excretion was established in Expt 1, using eighteen male Bos indicus×Bos taurus crossbred cattle (261 (se 9·1) kg body weight), six of each of the following genotypes: 5/8 Bos indicus, 1/2 Bos indicus and 3/8 Bos indicus. No significant differences were observed among genotypes in fasting purine derivative excretion (277·3 (se 35·43) μmol/metabolic body weight). In a second experiment we measured the xanthine oxidase activity, which was higher in liver than in duodenal mucosa (0·64 and 0·06 (se 0·12) units/g wet tissue per min respectively; P>0·05) being in plasma 0·60 (se 0·36) units/l per min. The kinetics of uric acid were measured by intravenous pulse dose of [1,3-15N]uric acid (Expt 3). The cumulative recovery of the isotope in urine was 82 (se 6·69) %, and uric acid plasma removal, pool size and mean retention time were 0·284 (se 0·051) per h, 5·45 (se 0·823) mmol and 3·52 (se 0·521) h, respectively. Allantoin was removed from plasma at an estimated fractional rate of 0·273 (se 0·081) per h and mean retention was 3·66 (se 1·08) h. In Expt 4, the relationship between urinary purine derivative excretion (Y; mmol/d) and digestible organic matter intake (X, kg/d) was defined by the equation: Y=7·69 (se 4·2)+5·69 (se 1·68) X; n 16, Se 1·31, r 0·67.

Keywords

Bos indicusPurine derivativesXanthine oxidase
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 6 , June 2005 , pp. 821 - 828
Copyright
Copyright © The Nutrition Society 2005

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