Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-08T19:32:42.648Z Has data issue: false hasContentIssue false
  • English
  • Français

Rumen simulation technique study on the interactions of dietary lauric and myristic acid supplementation in suppressing ruminal methanogenesis

Published online by Cambridge University Press:  09 March 2007

Carla R. Soliva ,
Leo Meile ,
Adam Cieślak ,
Michael Kreuzer  and
Andrea Machmüller
Carla R. Soliva*
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
Leo Meile
Affiliation:
Institute of Food Science and Nutrition, Laboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
Adam Cieślak
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland Department of Animal Nutrition and Feed Management, Agricultural University of Poznań, ul. Wolynska 33, 60-637 Poznań, Poland
Michael Kreuzer
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
Andrea Machmüller
Affiliation:
Institute of Animal Science, Animal NutritionLaboratory of Food Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zentrum/LFW, CH-8092 Zurich, Switzerland
*
*Corresponding author: Dr Carla R. Soliva, fax +41 1 632 1128, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The interactions of lauric (C12) and myristic acid (C14) in suppressing ruminal methanogenesis and methanogens were investigated with the rumen simulation technique (Rusitec) using bovine ruminal fluid. The fatty acids were added to basal substrates (grass hay:concentrate, 1:1.5) at a level of 48 g/kg DM, provided in C12:C14 ratios of 5:0, 4:1, 3:2, 2·5:2.5, 2:3, 1:4 and 0:5. Additionally, an unsupplemented control consisting of the basal substrates only was employed. Incubation periods lasted for 15 (n 4) and 25 (n 2) d. CH4 formation was depressed by any fatty acid mixture containing at least 40 % C12, and effects persisted over the complete incubation periods. The greatest depression (70 % relative to control) occurred with a C12:C14 ratio of 4:1, whereas the second most effective treatment in suppressing CH4 production (60 % relative to control) was found with a ratio of 3:2. Total methanogenic counts were decreased by those mixtures of C12 and C14 also successful in suppressing methanogenesis, the 4:1 treatment being most efficient (60 % decline). With this treatment in particular, the composition of the methanogenic population was altered in such a way that the proportion of Methanococcales increased and Methanobacteriales decreased. Initially, CH4 suppression was associated with a decreased fibre degradation, which, however, was reversed after 10 d of incubation. The present study demonstrated a clear synergistic effect of mixtures of C12 and C14 in suppressing methanogenesis, mediated probably by direct inhibitory effects of the fatty acids on the methanogens.

Keywords

MethaneMedium-chain fatty acidsLipidsRusitec
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 4 , October 2004 , pp. 689 - 700
Copyright
Copyright © The Nutrition Society 2004

References

Ababouch, L, Chaibi, A & Busta, FF (1992) Inhibition of bacterial spore growth by fatty acids and their sodium salts. J Food Protect 55, 980–984.CrossRefGoogle ScholarPubMed
Abel, HJ, Immig, I & Harmann, E (2002) Effect of adding caprylic and capric acid to grass on fermentation characteristics during ensiling and in the artificial rumen system RUSITEC. Anim Feed Sci Technol 99, 65–72.CrossRefGoogle Scholar
Bergsson, G, Arnfinnsson, J, Karlsson, SM, Steingrimsson, O & Thormar, H (1998) In vitro inactivation of Chlamydia trachomatis by fatty acids and monoglycerides. Antimicrob Agents Chemother 42, 2290–2294.Google Scholar
Bergsson, G, Arnfinnsson, J, Steingrimsson, O & Thormar, H (2001) Killing of gram-positive cocci by fatty acids and monoglycerides. APMIS 109, 670–678.Google Scholar
Blackburn, TH & Hungate, RE (1963) Succinic acid turnover and propionate production in the bovine rumen. Appl Microbiol 11, 132–135.Google Scholar
Blaxter, KL & Czerkawski, J (1966) Modification of methane production of sheep by supplementation of its diet. J Sci Food Agric 17, 417–420.CrossRefGoogle ScholarPubMed
Carro, MP, Lebzien, P & Rohr, K (1995) Effect of pore size of nylon bags and dilution rate on fermentation parameters in a semi-continuous artificial rumen. Small Rum Res 15, 113–119.CrossRefGoogle Scholar
Czerkawski, JW & Breckenridge, G (1977) Design and development of a long-term rumen simulation technique (RUSITEC). Br J Nutr 38, 317–384.Google Scholar
Demeyer, DI (1991) Quantitative aspects of microbial metabolism in the rumen and hindgut.In Rumen Microbial Metabolism and Ruminant Digestion, 217–237 [Jouany, JP, editors]. Paris, France: INRA Editions.Google Scholar
Dohme, F, Machmüller, A, Estermann, BL, Pfister, P, Wasserfallen, A & Kreuzer, M (1999) The role of the rumen ciliate protozoa for methane suppression caused by coconut oil. Lett Appl Microbiol 29, 187–192.Google Scholar
Dohme, F, Machmüller, A, Wasserfallen, A & Kreuzer, M (2000) Comparative efficiency of various fats rich in medium-chain fatty acids to suppress ruminal methanogenesis as measured with Rusitec. Can J Anim Sci 80, 473–482.Google Scholar
Dohme, F, Machmüller, A, Wasserfallen, A & Kreuzer, M (2001 a) Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets. Lett Appl Microbiol 32, 47–51.CrossRefGoogle ScholarPubMed
Dohme, F, Sutter, F, Machmüller, A & Kreuzer, M (2001 b) Methane formation and energy metabolism of lactating cows receiving individual medium-chain fatty acids Energy Metabolism in Animals. Proceedings of the 15th Symposium on Energy Metabolism in Animals, 2000, 369–372 [Chwalibog, AJabobsen, K, editors]. Wageningen, The Netherlands: Pers Wageningen.Google Scholar
Dong, Y, Bae, HD, McAllister, TA, Mathison, GW, Cheng, K-J (1997) Lipid-induced depression of methane production and digestibility in the artificial rumen system (RUSITEC). Can J Anim Sci 77, 269–278.CrossRefGoogle Scholar
Finlay, BJ, Esteban, G, Clarke, KJ, Williams, AG, Embley, TM & Hirt, RP (1994) Some rumen ciliates have endosymbiotic methanogens. FEMS Microbiol Lett 117, 157–162.CrossRefGoogle ScholarPubMed
Harfoot, CG, Crouchman, ML, Noble, RC & Moore, JH (1974) Competition between food particles and rumen bacteria in the uptake of long chain fatty acids and triglycerides. J Appl Bact 37, 633–641.Google Scholar
Henderson, C (1973) The effect of fatty acids on pure cultures of rumen bacteria. J Agric Sci 81, 107–112.CrossRefGoogle Scholar
Hino, T & Asanuma, N (2003) Suppression of ruminal methanogenesis by decreasing the substrates available to methanogenic bacteria. Nutr Abstr Rev 73, 1–8.Google Scholar
Jenkins, TC (1993) Lipid metabolism in the rumen. J Dairy Sci 76, 3851–3863.CrossRefGoogle ScholarPubMed
Jenkins, TC & Palmquist, DL (1984) Effect of fatty acids or calcium soaps on rumen and total nutrient digestibility of dairy rations. J Dairy Sci 67, 978–986.Google Scholar
Jouany, JP (1994) Manipulation of microbial activity in the rumen. Arch Anim Nutr 46, 133–153.Google ScholarPubMed
Kabara, JJ (1993) Medium-chain fatty acids and esters.In Antimicrobials in Foods, 307–342 [Davidson, PMBranen, AL, editors]. New York: Marcel Dekker.Google Scholar
Kabara, JJ & Vrable, R (1977) Antimicrobial lipids: natural and synthetic fatty acids and monoglycerides. Lipids 12, 753–759.Google Scholar
Khalil, MAK (2000) Atmospheric methane: an introduction Atmospheric Methane: Its Role in the Global Environment, 1–8 [Khali, MAK, editors]. Berlin, Germany: Springer-Verlag.Google Scholar
Koster, IW & Cramer, A (1987) Inhibition of methanogenesis from acetate in granular sludge by long-chain fatty acids. Appl Environ Microbiol 53, 403–409.Google Scholar
Krause, DO, Denman, SE, Mackie, RI, Morrison, M, Rae, AL, Attwood, GT & McSweeney, CS (2003) Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics. FEMS Microbiol Rev 27, 663–693.CrossRefGoogle ScholarPubMed
Le Van, TD, Robinson, LA, Ralph, J, Greening, RC, Smolenski, WJ, Leedle, JAZ & Schaefer, DM (1998) Assessment of reductive acetogenesis with indigenous ruminal bacterium populations and Acetitomaculum ruminis. Appl Environ Microbiol 64, 3429–3436.CrossRefGoogle ScholarPubMed
Lin, C, Raskin, L & Stahl, DA (1997) Microbial community structure in gastrointestinal tracts of domestic animals: comparative analyses using rRNA-targeted oligonucleotide probes. FEMS Microbiol Ecol 22, 281–294.CrossRefGoogle Scholar
Littell, RC, Henry, PR & Ammerman, CB (1998) Statistical analysis of repeated measures data using SAS procedures. J Anim Sci 76, 1216–1231.Google Scholar
Machmüller, A & Kreuzer, M (1999) Methane suppression by coconut oil and associated effects on nutrient and energy balance in sheep. Can J Anim Sci 79, 65–72.CrossRefGoogle Scholar
Machmüller, A, Ossowski, DA, Wanner, M & Kreuzer, M (1998) Potential of various fatty feeds to reduce methane release from rumen fermentation in vitro (Rusitec). Anim Feed Sci Technol 71, 117–130.Google Scholar
Machmüller, A, Soliva, CR & Kreuzer, M (2002) In vitro ruminal methane suppression by lauric acid as influenced by dietary calcium. Can J Anim Sci 82, 233–239.Google Scholar
Machmüller, A, Soliva, CR & Kreuzer, M (2003) Methane-suppressing effect of myristic acid in sheep as affected by dietary calcium and forage proportion. Br J Nutr 90, 529–540.Google Scholar
Matsumoto, M, Kobayashi, T, Takenaka, A & Itabashi, H (1991) Defaunation effects of medium-chain fatty acids and their derivatives on goat rumen protozoa. J Gen Appl Microbiol 37, 439–445.CrossRefGoogle Scholar
McLay, JC, Kennedy, MJ, O'Rourke, AL, Elliot, RM & Simmonds, RS (2002) Inhibition of bacterial foodborne pathogens by the lactoperoxidase system in combination with monolaurin. Int J Food Microbiol 73, 1–9.Google Scholar
Miller, TL (1995) Ecology of methane production and hydrogen sink in the rumen.In Ruminant Physiology: Digestion, Metabolism, Growth and Reproduction, 317–331 [Engelhardt, WBreves, GLeonhard-Marek, SGiesecke, D, editors]. Berlin, Germany: Ferdinand Enke Verlag.Google Scholar
Morvan, B, Bonnemoy, F, Fonty, G & Gouet, P (1996) Quantitative determination of H 2 -utilizing acetogenic and sulfate-reducing bacteria and methanogenic archaea from digestive tract of different mammals. Curr Microbiol 32, 129–133.Google Scholar
Naumann, K & Bassler, R (1997) Die chemische Untersuchung von Futtermitteln Methodenbuch 4th ed., 1–4Darmstadt, Germany: VDLUFA-Verlag.Google Scholar
Raskin, L, Stromley, JM, Rittmann, BE & Stahl, DA (1994) Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. Appl Environ Microbiol 60, 1232–1240.CrossRefGoogle ScholarPubMed
Sharp, R, Ziemer, CJ, Stern, MD & Stahl, DA (1998) Taxon-specific associations between protozoal and methanogen populations in the rumen and a model rumen system. FEMS Microbiol Ecol 26, 71–78.Google Scholar
Soliva, CR, Hess, HD, Meile, L, Kreuzer, M, Machmüller, A (2003 a) Suppression of ruminal methanogenesis by dietary means: apparent inconsistency between methane release and counts of microbes involved in methanogenesis. Tropic Subtropic Agroecosyst 3, 209–213.Google Scholar
Soliva, CR, Hindrichsen, IK, Meile, L, Kreuzer, M, Machmüller, A (2003 b) Effects of mixtures of lauric and myristic acid on rumen methanogens and methanogenesis in vitro. Lett Appl Microbiol 37, 35–39.Google Scholar
Stahl, DA & Amann, R (1991) Development and application of nucleic acid probes.In Nucleic Acid Techniques in Bacterial Systematics, 205–248 [Stackebrandt, EGoodfellow, M, editors]. New York: John Wiley &xs Sons Inc.Google Scholar
Stahl, DA, Amann, RI, Poulsen, LK, Raskin, L & Capman, WC (1995) Use of fluorescent probes for determinative microscopy of methanogenic archaea.In Archaea: Methanogens: A Laboratory Manual, 111–121 [Sowers, KRSchreier, HJ, editors]. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Tangerman, A & Nagengast, FM (1996) A gas chromatographic analysis of fecal short-chain fatty acids, using the direct injection method. Anal Biochem 236, 1–8.Google Scholar
Thormar, H, Isaacs, CE, Brown, HR, Barshatzky, MR & Pessolano, T (1987) Inactivation on enveloped viruses and killing of cells by fatty acids and monoglycerides. Antimicrob Agents Chemother 31, 27–31.Google Scholar
Van Nevel, CJ & Demeyer, DI (1995) Feed additives and other interventions for decreasing methane emissions.In Biotechnology in Animal Feeds and Animal Feeding, 329–349 [Wallace, RJChesson, A, editors]. Weinheim, Germany: VCH.Google Scholar
Whitelaw, FG, Eadie, JM, Bruce, IA & Shand, WJ (1984) Methane formation in faunated and ciliate-free cattle and its relationship with rumen volatile fatty acid proportions. Br J Nutr 52, 261–275.CrossRefGoogle ScholarPubMed
Wolin, MJ, Miller, TL & Stewart, CS (1997) Microbe-microbe interactions.In The Rumen Microbial Ecosystem, 467–491 [Hobson, PNStewart, CS, editors]. London: Chapman and Hall.Google Scholar
Wuebbles, DJ & Hayhoe, K (2002) Atmospheric methane and global change. Earth-Sci Rev 57, 117–210.CrossRefGoogle Scholar