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The retention and metabolism of Nτ-methylhistidine by cockerels: implications for the measurement of muscle protein breakdown determined from the excretion of Nτmethylhistidine in excreta

Published online by Cambridge University Press:  09 March 2007

C. I. Harris ,
G. Milne  and
Ruth McDiarmid
C. I. Harris
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
G. Milne
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Ruth McDiarmid
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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1. Excreta were collected for four consecutive days from 4- to 18-week-old cockerels following subcutaneous injection of Nτ-[14CH3]methylhistidine.

2. The recoveries of radioactivity in excreta were incomplete and progressively decreased with increasing age.

3. Most of the radioactivity not recovered in excreta after 4 d was found in skeletal muscle where > 55% of the radioactivity present was in the Nτ-methylhistidine-containing dipeptide, balenine.

4. This peptide appeared to be relatively stable so that most of the labelled Nτ-methylhistidine incorporated was not released during the period of the recovery measurements.

5. The total pool of non-protein bound Nτ-methylhistidine (free Nτ-methylhistidine+balenine) in pectoral and mixed thigh muscles increased with age and relative to the daily excretion of Nτ-methylhistidine. At 18 weeks the pool was 3.3 times the daily excretion of Nτ-methylhistidine.

6. These observations account for the decreasing recoveries of radioactivity in excreta described previously, due to progressive dilution of labelled Nτ-methylhistidine in an expanding pool of non-protein-bound Nτ-methylhistidine, part of which was relatively stable.

7. It is concluded that excretion of Nτ-methylhistidine by 4- to 18-week-old cockerels cannot be used as a reliable index of muscle protein breakdown in vivo.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 57 , Issue 3 , May 1987 , pp. 467 - 478
Copyright
Copyright © The Nutrition Society 1987

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