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Restoration of the glycogen-forming function of hepatocytes in rats with liver cirrhosis is facilitated by a high-carbohydrate diet

Published online by Cambridge University Press:  09 March 2007

Margarita V. Kudryavtseva ,
Natalia N. Besborodkina  and
Boris N. Kudryavtsev
Margarita V. Kudryavtseva*
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
Natalia N. Besborodkina
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
Boris N. Kudryavtsev
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
*
*Corresponding author: Dr Margarita Kudryavtseva, fax +7 812 247 0341, email [email protected]
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Abstract

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Using cytofluorimetric and biochemical methods, the content of glycogen and its labile and stable fractions, as well as activities of glucose-6-phosphatase (EC 3.1.3.9), glycogen phosphorylase (EC 2.4.1.1) and glycogen synthase (EC 2.4.1.11) were determined in the rat liver for 6 months after chronic poisoning of the animals with CCl4 and then at 1, 3, and 6 months after the end of the poisoning. One group of rats was given a standard diet, the other, a high-carbohydrate diet. The 6-month long chronic intoxication with CCl4 was shown to produce development of typical liver cirrhosis characterized by a 2·8-fold increase in the total glycogen content in hepatocytes as compared with normal cells, by a fall in the glycogen labile fraction (from 85 to 53 % of the total glycogen) as well as by decreases in the activities of glycogen phosphorylase and glucose-6-phosphatase by 25 and 82% respectively. The structural rehabilitation occurred faster and more completely at the cellular level than at the tissue level. Functional variables of the cirrhotic liver tissue also recovered, after cessation of poisoning, faster and more completely than the liver structure at the tissue level: glycogen levels in hepatocytes fell dramatically, the labile: stable glycogen fraction ratio recovered completely, and the activity of glycogen phosphorylase rose to the level characteristic of the normal liver. Use of the high-carbohydrate diet promoted a somewhat faster and more complete recovery of hepatic structure and function.

Keywords

Liver cirrhosisGlycogenHigh-carbohydrate diet
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 6 , June 1999 , pp. 473 - 480
Copyright
Copyright © The Nutrition Society 1999

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