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Protective effects of tomato extract with elevated β-carotene levels on oxidative stress in ARPE-19 cells

Published online by Cambridge University Press:  08 March 2007

Gurunadh R. Chichili ,
Donatus Nohr ,
Jürgen Frank ,
Andrea Flaccus ,
Paul D. Fraser ,
Eugenia M. A. Enfissi  and
Hans K. Biesalski
Gurunadh R. Chichili
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, University of Hohenheim, D-70599 Stuttgart, Germany
Donatus Nohr
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, University of Hohenheim, D-70599 Stuttgart, Germany
Jürgen Frank
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, University of Hohenheim, D-70599 Stuttgart, Germany
Andrea Flaccus
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, University of Hohenheim, D-70599 Stuttgart, Germany
Paul D. Fraser
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
Eugenia M. A. Enfissi
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
Hans K. Biesalski*
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, University of Hohenheim, D-70599 Stuttgart, Germany
*
*Corresponding author: Professor H. K. Biesalski, fax +49 711 459 3822, email [email protected]
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Abstract

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Epidemiological studies show that dietary products rich in carotenoids delay the progression of age-related macular degeneration. Experimental evidence from cellular studies on the antioxidant actions of carotenoids in the retinal pigment epithelium is still, however, fragmentary. The present study examined the uptake and protective potential of dietary carotenoids from tomato on the human retinal pigment epithelial cell line ARPE-19. ARPE-19 cells were incubated in medium supplemented with tomato extract containing high levels of ß-carotene, lycopene and traces of lutein. The cellular uptake of carotenoids was analysed by reverse-phase HPLC. Oxidative stress was induced by treatment with 1 mm-H2O2. Nitrotyrosine was detected by immunocytochemistry, and oxidised proteins (protein carbonyls) were measured by a quantitative ELISA method. Lipid peroxidation was assessed by quantifying thiobarbituric acid reactive substances. ARPE-19 cells preferentially accumulated lutein and ß-carotene rather than lycopene. Nitrotyrosine formation was considerably reduced in cells incubated with tomato extract compared with controls after H2O2 treatment. Protein carbonyls were reduced by 30 % (P=0·015), and the formation of thiobarbituric acid-reactive substances was reduced by 140 % (P=0·003) in cells incubated with tomato extract. The present study provides the experimental evidence for protective effects of dietary tomatoes rich in carotenoids on oxidative stress in the retinal pigment epithelium.

Keywords

ß-caroteneOxidative stressRetinaAMDRetinal pigment epitheliumTomatoCarotenoids
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 4 , October 2006 , pp. 643 - 649
Copyright
Copyright © The Nutrition Society 2006

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