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Preparation and characterization of a [14C]cellulose suitable for human metabolic studies

Published online by Cambridge University Press:  09 March 2007

M. P. Walters ,
J. Kelleher ,
J. M. Findlay  and
S. T. Srinivasan
M. P. Walters
Affiliation:
Department of Medicine, St James's University Hospital, Leeds LS9 7TF
J. Kelleher
Affiliation:
Department of Medicine, St James's University Hospital, Leeds LS9 7TF
J. M. Findlay
Affiliation:
Department of Gastroenterology, Bradford Royal Infirmary, Bradford
S. T. Srinivasan
Affiliation:
Department of Medicine, St James's University Hospital, Leeds LS9 7TF
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Abstract

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In six normal subjects administered 5 μCi of an oral dose of a commercially available 14C-labelled cellulose, significant amounts of 14CO2 were detected in expired air within 30 min, suggesting that other 14C-labelled non-cellulosic material was present. Chemical and microscopical examination confirmed that starch was the principal contaminant. The commercial preparation was purified using amyloglucosidase (EC 3.2.1.3) digestion following gelatinization of the starch by autoclaving. Subsequent administration of the purified cellulose to a further six normal subjects decreased the expired air 14CO2 during the subsequent 10 h from 13.0 (sd 4.0) to 4.1 (sd 1.9)%. Administration of the purified product to a further group of four normal subjects, before and after a regimen of increased dietary fibre, showed a cumulative increase in expired 14CO2 over 24 h from 7.9 (sd 1.1) to 12.1 (sd 2.6) % on fibre. In six ileostomy subjects the cumulative excretion of 14CO2 was greatly decreased compared with normal controls (3.0 (sd 1.14) and 10.5 (sd 3.9) % respectively). In constipated subjects expired 14CO2 continued beyond 48 h, in contrast to normal subjects where expired 14CO2 at this time was negligible.

Keywords

Dietary fibreDigestion of celluloseHuman metabolic studies
Type
Research Article
Information
British Journal of Nutrition , Volume 62 , Issue 1 , July 1989 , pp. 121 - 129
Copyright
Copyright © The Nutrition Society 1989

References

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