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Phyto-oestrogen content of berries, and plasma concentrationsand urinary excretion of enterolactone after asingle strawberry-meal in human subjects

Published online by Cambridge University Press:  09 March 2007

W. M. Mazur ,
M. Uehara ,
K. Wähälä  and
H. Adlercreutz
W. M. Mazur
Affiliation:
Folkhälsan Institute for Preventive Medicine, Nutrition and Cancer and Department of Clinical Chemistry, University of Helsinki, PO Box 60, FIN-00014 Helsinki, Finland
M. Uehara
Affiliation:
Folkhälsan Institute for Preventive Medicine, Nutrition and Cancer and Department of Clinical Chemistry, University of Helsinki, PO Box 60, FIN-00014 Helsinki, Finland
K. Wähälä
Affiliation:
Folkhälsan Institute for Preventive Medicine, Nutrition and Cancer and Department of Clinical Chemistry, University of Helsinki, PO Box 60, FIN-00014 Helsinki, Finland
H. Adlercreutz*
Affiliation:
Folkhälsan Institute for Preventive Medicine, Nutrition and Cancer and Department of Clinical Chemistry, University of Helsinki, PO Box 60, FIN-00014 Helsinki, Finland
*
*Corresponding author: Professor Herman Adlercreutz, fax +358 9 61585633, email [email protected]
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Abstract

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Quantitative data on phyto-oestrogen, particularly lignan, content in edible plants are insufficient. We, therefore, measured isoflavonoids and lignans in nine edible berries using an isotope dilution gas chromatography–mass spectrometry method for foods and found substantial concentrations of the lignan secoisolariciresinol (1·39–37·18 mg/kg DM), low amounts of matairesinol (0–0·78 mg/kg DM) and no isoflavones. To determine pharmacokinetics and urinary excretion pattern of the mammalian lignan enterolactone derived from plant lignans, a study with human subjects was conducted. Five healthy women and two men consumed, after a 72 h period of a phyto-oestrogen-free regimen, a single strawberry-meal containing known amounts of plant lignans. Basal and post-meal blood and urine samples were collected at short intervals. The samples were analysed using time-resolved fluoroimmunoassay of enterolactone. The meal increased plasma concentration of enterolactone after 8–24 h and in urine in the 13–24 h and 25–36 h urine collections. High individual variability of the metabolic response was observed. Enterolactone excreted in the urine collected throughout the 48 h post-meal yielded on average 114 % of the plant lignans consumed. It is concluded that berries containing relatively high concentrations of plant lignans contribute to plasma and urinary levels of mammalian enterolactone in human subjects.

Keywords

BerriesPhyto-oestrogensKinetics
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 4 , April 2000 , pp. 381 - 387
Copyright
Copyright © The Nutrition Society 2000

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