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Oxidative stress in marathon runners: interest of antioxidant supplementation

Published online by Cambridge University Press:  08 March 2007

Mari-Carmen Gomez-Cabrera ,
Agustín Martínez ,
Gustavo Santangelo ,
Federico V. Pallardó ,
Juan Sastre  and
Jose Viña
Mari-Carmen Gomez-Cabrera
Affiliation:
Catholic University of Valencia, Spain
Agustín Martínez
Affiliation:
Department of Physiology, University of Valencia, School of Medicine, Blasco Ibañez 15, E-46010 Valencia
Gustavo Santangelo
Affiliation:
Department of Physiology, University of Valencia, School of Medicine, Blasco Ibañez 15, E-46010 Valencia
Federico V. Pallardó
Affiliation:
Department of Physiology, University of Valencia, School of Medicine, Blasco Ibañez 15, E-46010 Valencia
Juan Sastre
Affiliation:
Department of Physiology, University of Valencia, School of Medicine, Blasco Ibañez 15, E-46010 Valencia
Jose Viña*
Affiliation:
Department of Physiology, University of Valencia, School of Medicine, Blasco Ibañez 15, E-46010 Valencia
*
*Corresponding author: Dr Jose Viña, fax +34 96 386 46 42, email [email protected]
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Abstract

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We have recently reported that xanthine oxidase is involved in the generation of free radicals in exhaustive exercise. Allopurinol, an inhibitor of xanthine oxidase, prevents it. The aim of the present work was to elucidate the role of exercise-derived reactive oxygen species in the cell signalling pathways involved in the adaptation to exercise in man. We have found that exercise causes an increase in the activity of plasma xanthine oxidase and an activation of NF-κB in peripheral blood lymphocytes after marathon running. This activation is dependent on free radical formation in exercise: treatment with allopurinol completely prevents it. In animal models, we previously showed that NF-κB activation induced by exhaustive physical exercise leads to an increase in the expression of superoxide dismutase, an enzyme involved in antioxidant defence. We report evidence in man that reactive oxygen species act as signals in exercise as decreasing their formation prevents activation of important signalling pathways which can cause useful adaptations in cells.

Keywords

NF-κBReactive oxygen speciesSignal transductionMarathon runners
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue S1 , August 2006 , pp. S31 - S33
Copyright
Copyright © The Nutrition Society 2006

References

Baeuerle, PA & Baltimore, D (1988) Activation of DNA binding activity in an apparently cytoplasmic precursor of the NF-κB transcriptional factor. Cell 53, 211–217.CrossRefGoogle Scholar
Davies, KJA, Quintanilha, AT, Brooks, GA & Packer, L (1982) Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun 107, 1198–1205.CrossRefGoogle ScholarPubMed
Gomez-Cabrera, MC, Pallardo, FV, Sastre, J, Viña, J, Garcia-del-Moral, L (2003) Allopurinol and markers of muscle damage among participants in the Tour de France. JAMA 289, 2503–2504.CrossRefGoogle ScholarPubMed
Harris, R & Ukaejiofo, EO (1970) Tissue typing using a routine one-step lymphocyte separation procedure. Br J Haematol 18, 229–235.CrossRefGoogle ScholarPubMed
Heunks, LM, Viña, J, van Herwaarden, CL, Folgering, HT, Gimeno, A & Dekhuijzen, PN (1999) Xanthine oxidase is involved in exercise-induced oxidative stress in chronic obstructive pulmonary disease. Am J Physiol 277, 1697–1704.Google ScholarPubMed
Hollander, J, Fiebig, R, Gore, M, Ookawara, T, Ohno, H & Ji, LL (2001) Superoxide dismutase gene expression is activated by a single bout of exercise in rat skeletal muscle. Pflugers Arch 442, 426–434.CrossRefGoogle ScholarPubMed
Ji, LL, Gomez-Cabrera, MC, Steinhafel, N, Viña, J (2004) Acute exercise activates nuclear factor (NF) κB signaling pathway in rat skeletal muscle. FASEB J 18   1500–1506.CrossRefGoogle ScholarPubMed
Khassaf, M, McArdle, A & Esanu, C et al. (2003) Effect of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle.   J Physiol 549, 645–652.Google ScholarPubMed
Reid, MB (2001) Redox modulation of skeletal muscle contraction: what we know and what we don't. J Appl Physiol 90, 724–731.CrossRefGoogle ScholarPubMed
Salminen, A, Kainulainen, H & Vihko, V (1984) Endurance training and antioxidants of lung. Experientia 15, 822–823.CrossRefGoogle Scholar
Sastre, J, Asensi, N & Gascó, E et al. (1992) Exhaustive physical exercise causes oxidation of glutathione status in blood: prevention by antioxidant administration. Am J Physiol 32, R992–R995.Google Scholar
Vider, J, Laaksonen, DE & Kilk, A et al. (2001) Physical exercise induces activation of NF-κB in human peripheral blood lymphocytes. Antioxid Redox Signal 3,   1131–1137.CrossRefGoogle ScholarPubMed
Viña, J, Gimeno, A & Sastre, J et al. (2000 a) Mechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol. IUBMB Life 49, 539–544.CrossRefGoogle ScholarPubMed
Viña, J, Gomez-Cabrera, MC & Lloret, A et al. (2000 b) Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. IUBMB Life 50, 271–277.CrossRefGoogle ScholarPubMed
Young, SHY, Knight, JA, Hopfer, SM, Zaharia, O, Leach, CN & Sunderman, FW (1987) Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde–thiobarbituric acid adduct. Clin Chem 33, 214–220.Google Scholar