Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-12-02T21:29:14.268Z Has data issue: false hasContentIssue false
  • English
  • Français

Octacosanol affects lipid metabolism in rats fed on a high-fat diet

Published online by Cambridge University Press:  09 March 2007

S. Kato ,
K.-I. Karino ,
S. Hasegawa ,
J. Nagasawa ,
A. Nagasaki ,
M. Eguchi ,
T. Ichinose ,
K. Tago ,
H. Okumori  and
K. Hamatani
...Show all authors
S. Kato
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
K.-I. Karino
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
S. Hasegawa
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
J. Nagasawa
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
A. Nagasaki
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
M. Eguchi
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
T. Ichinose
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
K. Tago
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
H. Okumori
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
K. Hamatani
Affiliation:
Sumitomo Seika Chemicals Co. L., Chuoku, Osaka, 541, Japan
M. Takahashi
Affiliation:
Sumitomo Seika Chemicals Co. L., Chuoku, Osaka, 541, Japan
J. Ogasawara
Affiliation:
Sumitomo Seika Chemicals Co. L., Chuoku, Osaka, 541, Japan
S. Masushige
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
S. Masushige
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagayaku, Tokyo, 156, Japan
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of dietary octacosanol, a long-chain alcohol, on lipid metabolism was investigated in rats fed on a high-fat diet for 20 d. The addition of octacosanol (10 g/kg diet) to the high-fat diet led to a significant reduction (P < 0·05) in the perirenal adipose tissue weight without decrease of the cell number, suggesting that octacosanol may suppress lipid accumulation in this tissue, whereas no effect was seen in the epididymal adipose tissue weight and in the lipid content in liver. Octacosanol supplementation decreased the serum triacylglycerol concentration, and enhanced the concentration of serum fatty acids, probably through inhibition of hepatic phosphatidate phosphohydrolase (EC 3·1·3·4). Though the activity of hormone-sensitive lipase (EC 3·1·1·3) was not influenced by octacosanol, higher activities of lipoprotein lipase (EC 3·1·1·34) in the perirenal adipose tissue and the total oxidation rate of fatty acid in muscle were observed. Lipid absorption was not affected by the inclusion of octacosanol. Thus, the present results suggest that the dietary incorporation of octacosanol into a high-fat diet affects some aspects of lipid metabolism.

Keywords

OctacosanolLipid metabolismAdipose tissueSerum triacylglycerolRat
Type
Effects of octacosanol on lipid metabolism
Information
British Journal of Nutrition , Volume 73 , Issue 3 , March 1995 , pp. 433 - 441
Copyright
Copyright © The Nutrition Society 1995

References

Cureton, T. K. (1972). Forty-two physiological training programs utilizing 894 humans. In The Physiological Effects of Wheat Germ Oil on Humans in Exercise, pp. 525–715 [Thomas, C. C. editor]. Illinois: Springfield.Google Scholar
Deshaies, Y., Arnold, J., Lalonde, J. & Richard, D. (1988). Lipoprotein lipase in white and brown adipose tissues of exercised rats fed a high-fat diet. American Journal of Physiology 255, R226–R231.Google Scholar
Dupont, J. (1965). Relationship between utilization of fat and synthesis of cholesterol and total lipid in young female rats. Journal of the American Oil Chemists' society 42, 903–909.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Gasquet, de P. & Pequignot, E. (1972). Lipoprotein lipase activities in adipose tissues, heart and diaphragm of the genetically obese mouse (ob/ob). Biochemical Journal 127, 445–447.CrossRefGoogle ScholarPubMed
Germershausen, J. I., Yudkovitz, J. B. & Greenspan, M. D. (1980). A sensitive assay for phosphatidate phosphohydrolase in mouse liver microsomes. Biochemica et Biophysica Acta 620, 562–571.CrossRefGoogle ScholarPubMed
Hamatani, K. & Takahashi, M. (1988). (Extraction from natural material by supercritical carbon dioxide and the commercialization of this extract). Zairyo Gijutsu 6, 325–329.Google Scholar
Hamatani, K., Takahashi, M., Ogasawara, J. & Inada, S. (1991). Recovery of octacosanol with supercritical CO2 by the two stage separation on the commercial scale. 2nd International Symposium on Supercritical Fluids (Abs), 6–9 [McHugh, M. A. editor]. Baltimore: Johns Hopkins University.Google Scholar
Kato, S., Mano, H., Kumazawa, T., Yoshizawa, Y., Kojima, R. & Masushige, S. (1992). Effect of retinoid status on α, β and y retinoic acid receptor mRNA levels in various rat tissues. Biochemical Journal 286, 755–760.Google Scholar
Kawada, T., Hagiwara, K. & Iwai, K. (1986). Effects of capsaicin on lipid metabolism in rats fed a high fat diet. Journal of Nutrition 116, 1272–1278.Google Scholar
Kornberg, A. & Horecker, B. L. (1955). Glucose-6-phosphate dehydrogenase. In Methods in Enzymology, Vol. 1, pp. 323–327 [Colowick, S. P. and Kaplan, N. O., editors]. New York: Academic Press.CrossRefGoogle Scholar
Lazarow, P. B. (1981). Assay of peroxysomal β-oxidation of fatty acids. In Methods in Enzymology, Vol. 72, pp. 315–319. [Lowenstein, J. M. editor]. New York: Academic Press.Google Scholar
Levin, E. (1963). Effects of octacosanol on chick comb growth. Proceedings of the Society for Experimental Biology and Medicine 112, 331–334.Google Scholar
Leyva, A. Jr & Kelley, W. N. (1974). Measurement of DNA in cultured human cells. Analytical Biochemistry 62, 173–179.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Numa, S., Bortz, W. M. & Lynen, F. (1965). Regulation of fatty acid synthesis at the acetyl-CoA carboxylation step. Advances in Enzyme Regulation 3, 407–423.CrossRefGoogle Scholar
Passwater, R. (1982). Octacosanol. Its name really spells increased energy, stamina and vigor. Health Quarterly 7, 14–15.Google Scholar
Reubsaet, F. A. G., Veerkamp, J. H., Dirven, H. A. A. M., BruckwilderM. L. H., M. L. H.,Hashimoto, T., Trijbels, J. M. F. & Monnens, L. A. H. (1990). The effect of di(ethyhexyl) phthalate on fatty acid oxidation and carnitine palmitoyltransferase in various rat tissues. Biochimica et Biophysica Acta 1047, 264–270.Google Scholar
Rizack, M. A. (1961). An epinephrine sensitive lipolytic activity in adipose tissue. Journal of Biological Chemistry 236, 657–662.CrossRefGoogle Scholar
Saint-John, M. & McNaughton, L. (1986). Octacosanol injection and its effects on metabolic responses to submaximal cycle ergometry, reaction time and chest and grip strength. International Clinical Nutrition Review 6, 81–87.Google Scholar
Shimura, S., Hasegawa, T., Takano, S. & Suzuki, T. (1987). Studies on the effect of octacosanol on motor endurance in mice. Nutrition Reports International 36, 1029–1038.Google Scholar
Smyth, D. H. (1974). Methods of studying intestinal absorption. In Biomembrane, Vol. 4A, pp. 241–283 [Smyth, D. H. editor], London: Plenum Press.Google Scholar
Tanabe, T., Nakanishi, S., Hashimoto, T., Ogiwara, H., Nikawa, J. & Numa, S. (1981). Acetyl CoA carboxylase from rat liver. In Methods in Enzymology, Vol. 71, pp. 5–16 [Lowenstein, J. M. editor]. New York: Academic Press.Google Scholar