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Microbial protein and peptide metabolism in rumen fluid from faunated and ciliate-free sheep

Published online by Cambridge University Press:  09 March 2007

R. J. Wallace ,
G. A. Broderick  and
Margaret L. Brammall
R. J. Wallace
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
G. A. Broderick
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Margaret L. Brammall
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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1. Protease and deaminase activities and the metabolism of peptides were measured in rumen fluid from ciliate- free sheep and from sheep with a limited population of small entodinia. The same measurements were repeated following inoculation of the latter group with a more typical mixed ciliate population.

2. Protease and dialanine uptake activities of mixed rumen micro-organisms were not significantly influenced by protozoa. Trialanine uptake, leucine aminopeptidase (EC 3.4.11. 1), deaminase and trypsin-like protease activities were 70, 107,73 and 91 % higher with the limited population, and 72,58,64 and 55 % higher when mixed protozoa were present, indicating a major role for the protozoa in these activities.

Type
General Nutrition papers
Information
British Journal of Nutrition , Volume 58 , Issue 1 , July 1987 , pp. 87 - 93
Copyright
Copyright © The Nutrition Society 1987

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