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Metabolic response to small and large 13C-labelled pasta meals following rest or exercise in man

Published online by Cambridge University Press:  09 March 2007

Nathalie Folch ,
François Péronnet ,
Denis Massicotte ,
Martine Duclos ,
Carole Lavoie  and
Claude Hillaire-Marcel
Nathalie Folch
Affiliation:
Département de kinésiologie, Université de Montréal, CP 6128 Centre-Ville, Montréal, Québec H3C 3J7, Canada
François Péronnet*
Affiliation:
Département de kinésiologie, Université de Montréal, CP 6128 Centre-Ville, Montréal, Québec H3C 3J7, Canada
Denis Massicotte
Affiliation:
Département de kinanthropologie and Laboratoire de géochimie isotopique et de géochronologie, Université du Québec à Montréal, Montréal, Québec H3C 3P8, Canada
Martine Duclos
Affiliation:
Laboratoire Neurogénétique et stress, INSERM U471, Institut François Magendie, rue Camille Saint Sëns, 33077 Bordeaux cedex, France
Carole Lavoie
Affiliation:
Université du Québec à Trois-Rivières, Trois-Rivières, Québec G9A 5H7, Canada
Claude Hillaire-Marcel
Affiliation:
Département de kinanthropologie and Laboratoire de géochimie isotopique et de géochronologie, Université du Québec à Montréal, Montréal, Québec H3C 3P8, Canada
*
*Corresponding author: François Péronnet, fax +1 514 343 2181, email [email protected]
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Abstract

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The metabolic response to a 150 or 400 g 13C-labelled pasta meal was studied for 8 h following rest or exercise at low or moderate workload (n 6). Following rest, the 400 g meal totally suppressed fat oxidation (v. 14.1 g following the 150 g meal) and a small amount of glucose was converted into fat (4.6 g), but fat oxidation remained high in subjects who had exercised following both the small (21.8 and 34.1 g) and large meal (14.1 and 32.3 g). Exogenous glucose oxidation was significantly higher in subjects who had remained at rest both following the small (67.6 g v. 60.4 and 51.3 g in subjects who exercised at low and moderate workloads) and large meal (152.2 v. 123.0 and 127.2 g). Endogenous glucose oxidation was similar in the three groups following the 150 g meal (42.3–58.0 g), but was significantly lower following the 400 g meal in subjects who had exercised at low workload (24.2 v. 72.2 g following rest; P<0.05), and was totally suppressed in those who had exercised at moderate workload. As a consequence, a larger positive glycogen balance was observed in subjects who exercised before the large meal (182.8–205.1 g v. 92.4 g following rest; P<0.05). Total fat oxidation calculated from 08.00 hours to 20.00 hours was similar in subjects who exercised at low and moderate workloads. These results indicate that: (1) de novo lipogenesis, which plays only a minor role for the disposal of an acute dietary carbohydrate load, is totally suppressed following exercise, even when a very large carbohydrate load is ingested; (2) the reduction in glycogen turnover as well as a preferential conversion of glucose into glycogen are responsible for the increase in glycogen stores following exercise; (3) for a similar energy expenditure, exercise at low workload for a longer period does not favour fat oxidation when the post-exercise period is taken into account.

Keywords

Stable isotopeDe novo lipogenesisGlycogen stores
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 6 , May 2001 , pp. 671 - 680
Copyright
Copyright © The Nutrition Society 2001

References

Aarsland, A, Chinkes, D & Wolfe, RR (1997) Hepatic and whole-body fat synthesis in humans during carbohydrate overfeeding. American Journal of Clinical Nutrition 65, 1774–1782.CrossRefGoogle ScholarPubMed
Acheson, KJ, Flatt, JP & Jéquier, E (1982) Glycogen synthesis versus lipogenesis after a 500 gram carbohydrate meal in man. Metabolism 31, 1234–1240.CrossRefGoogle ScholarPubMed
Acheson, KJ, Schutz, Y, Bessard, T, Anantharaman, K, Flatt, JP & Jéquier, E (1988) Glycogen storage capacity and de novo lipogenesis during massive carbohydrate overfeeding in man. American Journal of Clinical Nutrition 48, 240–247.CrossRefGoogle ScholarPubMed
Acheson, KJ, Schutz, Y, Bessard, T, Flatt, JP & Jéquier, E (1987) Carbohydrate metabolism and de novo lipogenesis in human obesity. American Journal of Clinical Nutrition 45, 78–85.CrossRefGoogle ScholarPubMed
Acheson, KJ, Schutz, Y, Bessard, T, Ravussin, E, Jéquier, E & Flatt, JP (1984) Nutritional influences on lipogenesis and thermogenesis after a carbohydrate meal. American Journal of Physiology 246, E62–E70.Google ScholarPubMed
Acheson, KJ, Thélin, A, Ravussin, E, Arnaud, MJ & Jéquier, E (1985) Contribution of 500 g naturally labeled 13C dextrin maltose to total carbohydrate utilization and the effect of the antecedent diet in man. American Journal of Clinical Nutrition 41, 881–890.CrossRefGoogle ScholarPubMed
Bielinski, R, Schutz, Y & Jéquier, E (1985) Energy metabolism during the postexercise recovery in man. American Journal of Clinical Nutrition 42, 69–82.CrossRefGoogle ScholarPubMed
Boirie, Y, Gachon, P, Corny, S, Fauquant, J, Maubois, JL & Beaufrère, B (1996) Acute postprandial changes in leucine metabolism as assessed with an intrinsically labeled milk protein. American Journal of Physiology 271, E1083–E1091.Google ScholarPubMed
Broeder, CE, Brenner, M, Hofman, Z, Paijmans, IJM, Thomas, EL & Wilmore, JH (1991) The metabolic consequences of low and moderate intensity exercise with or without feeding in lean and borderline obese males. International Journal of Obesity 15, 95–104.Google ScholarPubMed
Burelle, Y, Péronnet, F, Charpentier, S, Lavoie, C, Hillaire-Marcel, C & Massicotte, D (1999) Oxidation of an oral [13C]glucose load at rest and prolonged exercise in trained and sedentary subjects. Journal of Applied Physiology 86, 52–60.CrossRefGoogle ScholarPubMed
Calles-Escandón, J, Goran, MI, O'Connell, M, Nair, KS & Danforth, E (1996) Exercise increases fat oxidation at rest unrelated to changes in energy balance or lipolysis. American Journal of Physiology 270, E1009–E1014.Google ScholarPubMed
De #Garine, I & Koppert, GJA (1991) Guru-fattening sessions among the Massa. Ecology of Food and Nutrition 25, 1–28.CrossRefGoogle Scholar
Ebiner, JR, Acheson, KJ, Doerner, A, Maeder, E, Arnaud, MJ, Jéquier, E & Felber, JP (1979) Comparison of carbohydrate utilization in man using indirect calorimetry and mass spectrometry after an oral load of 100 g naturally-labelled [13C]glucose. British Journal of Nutrition 41, 419–429.CrossRefGoogle ScholarPubMed
Elia, M & Livesey, G (1988) Theory and validity of indirect calorimetry during net lipid synthesis. American Journal of Clinical Nutrition 47, 591–607.CrossRefGoogle ScholarPubMed
Faix, D, Neese, R, Kletke, C, Wolden, S, Cesar, D, Coutlangus, M, Shackleton, CHL & Hellerstein, MK (1993) Quantification of menstrual and diurnal periodicities in rates of cholesterol and fat synthesis in humans. Journal of Lipid Research 34, 2063–2075.CrossRefGoogle ScholarPubMed
Flatt, JP (1995) McCollum Award Lecture, 1995: Diet, lifestyle, and weight maintenance. American Journal of Clinical Nutrition 62, 820–836.CrossRefGoogle ScholarPubMed
Hellerstein, MK (1999) De novo lipogenesis in humans: metabolic and regulatory aspects. European Journal of Clinical Nutrition 53, S53–S65.CrossRefGoogle ScholarPubMed
Hellerstein, MK, Schwarz, JM & Neese, RA (1996) Regulation of hepatic de novo lipogenesis in humans. Annual Review of Nutrition 16, 523–557.CrossRefGoogle ScholarPubMed
Horton, TJ, Drougas, H, Brachey, A, Reed, GW, Peters, JC & Hill, JO (1995) Fat and carbohydrate overfeeding in humans: different effects on energy storage. American Journal of Clinical Nutrition 62, 19–29.CrossRefGoogle ScholarPubMed
Ivy, JL (1992) Resynthesis of muscle glycogen after exercise In Diabetes Mellitus and Exercise, pp. 153–164 [Devlin, JT, Horton, EF& Vranic, M, editors]. London: Smith Gordon.Google Scholar
Ivy, JL & Kuo, CH (1998) Regulation of GLUT4 protein and glycogen synthase during muscle glycogen synthesis after exercise. Acta Physiologica Scandinavica 162, 295–304.CrossRefGoogle ScholarPubMed
Jandrain, B, Krzentowski, G, Pirnay, F, Mosora, F, Lacroix, M, Luyckx, A & Lefèbvre, P (1984) Metabolic availability of glucose ingested 3 h before prolonged exercise in humans. Journal of Applied Physiology 56, 1314–1319.CrossRefGoogle ScholarPubMed
Jéquier, E (1992) Calorie balance versus nutrient balance. In Energy Metabolism: Tissue Determinants and Cellular Corollaries, 123–137. [JM, Kinney & HN, Tucker, editors]. New York: Raven Press.Google Scholar
Krzentowski, G, Pirnay, F, Luyckx, AS, Lacroix, M, Mosora, F & Lefèbvre, PJ (1983) Effect of physical training on the oxidation of an oral glucose load at rest: A naturally labeled 13C-glucose study. Diabète et Métabolisme 9, 112–115.Google Scholar
Krzentowski, G, Pirnay, F, Luyckx, AS, Pallikarakis, N, Lacroix, M, Mosora, F & Lefèbvre, PJ (1982) Metabolic adaptations in post-exercise recovery. Clinical Physiology 2, 277–288.CrossRefGoogle ScholarPubMed
Lefèbvre, PJ (1985) From plant physiology to human metabolic investigations. Diabetologia 28, 255–263.CrossRefGoogle ScholarPubMed
Livesey, G & Elia, M (1988) Estimation of energy expenditure, net carbohydrate utilization, and net fat oxidation and synthesis by indirect calorimetry: evaluation of errors with special reference to the detailed composition of fuels. American Journal of Clinical Nutrition 47, 608–628.CrossRefGoogle Scholar
Mikines, KJ, Farrell, PA, Sonne, B, Tronier, B & Galbo, H (1988 a) Postexercise dose–response relationship between plasma glucose and insulin secretion. Journal of Applied Physiology 64, 988–999.CrossRefGoogle ScholarPubMed
Mikines, KJ, Sonne, B, Farrell, PA, Tronier, B & Galbo, H (1988 b) Effect of physical exercise on sensitivity and responsiveness to insulin in humans. American Journal of Physiology 254, E248–E259.Google ScholarPubMed
Mosora, F, Lacroix, M, Luyckx, A, Pallikarakis, N, Pirnay, F, Krzentowski, G & Lefèbvre, P (1981) Glucose oxidation in relation to the size of the oral glucose loading dose. Metabolism 30, 1143–1149.CrossRefGoogle Scholar
Mosora, F, Lefèbvre, P, Pirnay, F, Lacroix, M, Luyckx, A & Duchesne, J (1976) Quantitative evaluation of the oxidation of an exogenous glucose load using naturally labeled 13C-glucose. Metabolism 25, 1575–1582.CrossRefGoogle ScholarPubMed
Normand, S, Pachiaudi, C, Khalfallah, Y, Guilluy, R, Mornex, R & Riou, JP (1992) 13C appearance in plasma glucose and breath CO2 during feeding with naturally 13C-enriched starchy food in normal humans. American Journal of Clinical Nutrition 55, 430–435.CrossRefGoogle ScholarPubMed
Péronnet, F, Massicotte, D, Brisson, G & Hillaire-Marcel, C (1990) Use of 13C substrates for metabolic studies in exercise: methodological considerations. Journal of Applied Physiology 69, 1047–1052.CrossRefGoogle ScholarPubMed
Phelain, JF, Reinke, E, Harris, MA & Melby, CL (1997) Postexercise energy expenditure and substrate oxidation in young women resulting from exercise bouts of different intensity. Journal of the American College of Nutrition 16, 140–146.CrossRefGoogle ScholarPubMed
Proserpi, C, Sparti, A, Schutz, Y, Di Vetta, V, Milon, H & Jéquier, E (1997) Ad libitum intake of a high-carbohydrate or high-fat diet in young men: effects on nutrient balances. American Journal of Clinical Nutrition 66, 539–545.CrossRefGoogle ScholarPubMed
Randle, PJ (1998) Regulatory interactions between lipids and carbohydrates: the glucose fatty acid cycle after 35 years. Diabetes Metabolism Review 14, 263–283.3.0.CO;2-C>CrossRefGoogle Scholar
Ravussin, E, Doerner, A, Acheson, KJ, Pahud, P, Arnaud, MJ & Jéquier, E (1980) Carbohydrate utilization in obese subjects after an oral load of 100 g naturally-labelled [13C] glucose. British Journal of Nutrition 43, 281–288.CrossRefGoogle ScholarPubMed
Schneiter, P, Di Vetta, V, Jéquier, E & Tappy, L (1995) Effect of physical exercise on glycogen turnover and net substrate utilization according to the nutritional state. American Journal of Physiology 269, E1031–E1036.Google Scholar
Thompson, DL, Townsend, KM, Boughey, R, Patterson, K & Bassett, DR (1998) Substrate use during and following moderate- and low-intensity exercise: Implications for weight control. European Journal of Applied Physiology 78, 43–49.CrossRefGoogle ScholarPubMed
Tipton, KD & Wolfe, RR (1998) Exercise-induced changes in protein metabolism. Acta Physiologica Scandinavica 162, 377–387.CrossRefGoogle ScholarPubMed
Truswell, AS (1992) Glycaemic index of foods. European Journal of Clinical Nutrition 46, S91–S101.Google ScholarPubMed
Wagenmakers, AJM (1998) Protein and amino acid metabolism in human muscle. Advances in Experimental Medicine and Biology 441, 307–319.CrossRefGoogle ScholarPubMed