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Marked alterations in circulating inflammatory cells during cardiomyopathy development in a magnesium-deficient rat model

Published online by Cambridge University Press:  09 March 2007

Joseph Kurantsin-Mills ,
Marie M. Cassidy ,
Richard E. Stafford  and
William B. Weglicki
Joseph Kurantsin-Mills
Affiliation:
Division of Hematology and Oncology, Department of Medicine, Department of Medicine, The George Washington University Medical Center, Washington, DC 20037, USA Department of Physiology, Department of Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
Marie M. Cassidy
Affiliation:
Department of Physiology, Department of Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
Richard E. Stafford
Affiliation:
Division of Experimental Medicine, Department of Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
William B. Weglicki
Affiliation:
Division of Experimental Medicine, Department of Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
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Abstract

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Rodents fed on a Mg-deficient (Mg-D) diet develop cardiomyopathic lesions, as well as other types of cardiovascular dysfunction. In the rat, inflammatory cell infiltration of the myocardium begins to occur by week 1, and the lesions develop extensively in the third and fourth weeks on the Mg-D diet. Although the aetiologic mechanisms of Mg-D cardiomyopathy are unknown, we have previously reported that once plasma Mg is markedly reduced, one of the earliest molecular markers of the pathophysiological process is elevation of plasma substance P, calcitonin gene-related peptide and prostaglandin E2, followed by histamine and the inflammatory cytokines (interleukin-1, interleukin-6, and tumor necrosis factor-α). In order to evaluate the potential role of specific circulating inflammatory cell subpopulations in the mechanisms underlying pathophysiological changes observed in Mg-deficiency-induced cardiomyopathy, we analysed these cells by flow cytochemistry. Leucocyte subpopulation pools increased progressively in the Mg-D rats. Elevated circulating levels of neutrophils and lymphocytes appeared to contribute to both the acute (week 1–2) and chronic phases (week 3–4) of the inflammatory responses; monocytes, eosinophils, basophils and large unstained cells which are lymphoid in stained smears, on the other hand, increased significantly in the third and fourth weeks and thus contributed to the chronic inflammatory phase. Changes in the circulating leucocyte subpopulations paralleled the chronological progression of the cardiomyopathic lesions, particularly in weeks 3 and 4. Since a pronounced neutrophilia preceded leucocyte infiltration and deposition within the myocardial tissue, modifications of the microvascular barrier may be a prerequisite for cardiomyopathy in this model of neurogenic inflammation.

Keywords

MagnesiumNeutrophiliaInflammatory cells
Type
General Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 5 , November 1997 , pp. 845 - 855
Copyright
Copyright © The Nutrition Society 1997

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