Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-18T17:33:33.441Z Has data issue: false hasContentIssue false
  • English
  • Français

Is gastric ulceration different in normal and malnourished rats?

Published online by Cambridge University Press:  08 March 2007

A. C. B. Paula ,
J. S. Gracioso ,
W. Toma ,
R. Bezerra ,
M. A. J. Saad ,
I. M. S. De Lucca ,
E. M. Carneiro  and
A. R. M. Souza Brito
A. C. B. Paula
Affiliation:
Department of Physiology and Biophysics, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil Department of Research and Graduate School (PRPPG), Sacred Heart University (USC), Bauru, São Paulo, Brazil
J. S. Gracioso
Affiliation:
Department of Physiology and Biophysics, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil
W. Toma
Affiliation:
Department of Physiology and Biophysics, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil
R. Bezerra
Affiliation:
Department of Internal Medicine, School of Medicine, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil
M. A. J. Saad
Affiliation:
Department of Internal Medicine, School of Medicine, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil
I. M. S. De Lucca
Affiliation:
Department of Histology and Embryology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil
E. M. Carneiro
Affiliation:
Department of Physiology and Biophysics, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil
A. R. M. Souza Brito*
Affiliation:
Department of Physiology and Biophysics, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil
*
*Corresponding author: A. R. M. Souza Brito, Departamento de Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil, fax +55(19)37886185, email [email protected], and [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Protein malnutrition can adversely affect all tissues. The aim of the present study was to test the hypothesis that protein deprivation influences gastric ulcer formation, as well as metabolism and organ growth, in rats. In the present study, there was a significant reduction in the body and organ weight of rats fed a low-protein diet (P<0·001). Malnourished rats were less susceptible to ulceration of the gastric mucosa in ethanol and indomethacin models of acute gastric ulcers when compared with rats fed a normoproteic diet (17 % protein). Mucus production and prostaglandin E2 formation increased in malnourished rats, possibly explaining the lower number of acute ulcers in these animals. Pylorus ligature altered gastric juice composition (increased pH and gastric volume, and decreased total acid concentration) in the animal group fed a low-protein diet compared with the group fed a diet containing 17 % protein (P<0·05). The gastric mucosa was more damaged in malnourished rats than in normal rats evaluated for 14 d after acetic acid injection (P<0·001). Malnourished rats exhibited resistance to acute gastric lesions, owing to an increase in prostaglandin GE2 release and mucus secretion, which protected their gastric mucosa. This phenomenon was not seen in subchronic gastric ulceration.

Keywords

Anti-ulcer activityMalnourishmentCytoprotectionGastric ulcers
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 1 , January 2005 , pp. 47 - 52
Copyright
Copyright © The Nutrition Society 2005

References

Bagchi, M, Milnes, M, Williams, C, Balmoori, J, Ye, X, Stahs, S & Bagchi, D (1999) Acute and chronic stress-induced oxidative gastrointestinal injury in rats, and the protective ability of a novel grape seed proanthocyanidin extract. Nutr Res 19, 1189–1199.CrossRefGoogle Scholar
Carneiro, EM, Mello, MAR, Gobatto, CA & Boschero, AC (1995) Low protein diet impairs glucose-induced insulin secretion from and 45 Ca uptake by pancreatic rat islets. J Nutr Biochem 6, 314–318.CrossRefGoogle Scholar
Curtis, GH, Macnaughton, K, Gall, DG & Wallace, JL (1995) Intraluminal pH modulates gastric prostaglandins synthesis. Can J Physiol Pharmacol 73, 130–134.CrossRefGoogle Scholar
Das, BK, Romesh, J, Agarwal, JK, Mishuro, OP & Bloatt, RP (1998) Blood glucose, serum insulin and corticosterone levels response in protein-malnourished rats. Infect Immun 66, 3562–3568.Google Scholar
Desai, M, Crowther, NJ, Lucas, A & Hales, CN (1996) Organ-selective growth in the offspring of protein-restricted mothers. Br J Nutr 76, 591–603.CrossRefGoogle ScholarPubMed
Diel, F & Szabo, S (1986) Dose-dependent effects of linear and cyclic somatostatin on ethanol-induced gastric erosions: the role of mast cells and increased vascular permeability in the rat. Regul Pept 13, 2735–2743.Google Scholar
D'Souza, RS & Dhume, VG (1991) Gastric cytoprotection. Indian J Physiol Pharmacol 35, 88–98.Google Scholar
Forsell, H (1988) Gastric mucosal defense mechanisms: a brief review. Scan J Gastroenterol Suppl 155, 23–28.CrossRefGoogle Scholar
Hales, CM & Barker, DJ (1992) Type 2 (non-insulin dependent) diabetes mellitus: the thrifty phenotype hypothesis. Diabetologia 35, 595–601.CrossRefGoogle ScholarPubMed
Hayden, LJ, Thomas, G & West, GB (1978) Inhibitors of gastric lesions in the rat. J Pharm Pharmacol 30, 244–246.Google Scholar
Karmeli, F, Eliakim, R, Okon, E & Rachmilewitz, D (1994) Gastric and mucosal damage by ethanol is mediated by substance P and prevented by ketotifen, a mast cell stabilizer. Gastroenterology 10, 1206–1216.Google Scholar
Klein, RM & McKenzie, JC (1993) The role of cell renewal in the ontogeny of the intestine. 1. Cell proliferation patterns in adult, fetal, and neonatal intestine. J Pediatric Gastroenterol Nutr 2, 1–43.Google Scholar
Konturek, SJ, Brzozowski, T, Taut, A, Sliwowski, Z, Stachura, J & Hahn, EG (1998) Activation of genes for growth factors and cyclooxygenase in gastric mucosa of rats during recovery from stress damage. Eur J Pharmacol 342, 55–65.CrossRefGoogle ScholarPubMed
Krous, SAL (1980) Research applications. In The Laboratory Rat. volume. II pp. 2–28 [Baker, HJ, Hussel, J, Weisbroth, SH, editors]. New York: Academic Press:Google Scholar
Latorraca, MQ, Carneiro, EM, Boschero, AC & Mello, MAR (1999) Reduced insulin secretion in response to nutrients in islets from malnourished young rats is associated with a diminished calcium uptake. J Nutr Biochem 10, 37–43.CrossRefGoogle ScholarPubMed
Lucas, A (1990) Does early diet programme future outcome?. Acta Paediatr Scand 365, 58–67.Google Scholar
Mattingly, DA (1962) A simple fluorimetric method for the estimation of free 11-hydroxycorticoids in human plasma. J Clin Path 15, 374–379.Google Scholar
Motilva, V, Martin, MJ, Luque, MI, De La Lastra, CA (1996) Role of polymorphonuclear leukocytes and oxyqen–derived free radicals in chronic gastric lesion induced by acetic acid in rat. Gen pharmac 27, 545–550.CrossRefGoogle ScholarPubMed
Morimoto, Y, Shimohara, K, Oshima, S & Sukamoto, T (1991) Effects of the new anti-ulcer agent KB-5492 on experimental gastric mucosal lesions and gastric mucosal defensive factors, as compared to those of terprenone and cimetidine. Jpn J Pharmacol 57, 495–505.Google Scholar
Okitolonda, W, Brichard, SM & Hunquin, JC (1987) Repercussions of chronic-calorie malnutrition on glucose homeostasis in rat. Diabetologia 30, 946–951.CrossRefGoogle Scholar
Olfert, ED, Cross, BM & McWilliam, AA (1993) Guide to the Care and Use of Experimental Animals, vol. 1, pp. 213. Ottawa, Ontario: Canadian Council on Animal Care.Google Scholar
Rafatullah, S, Tariq, M, Morra, JS, Al-Yahia, MA, Al-Sard, MS & Ageel, AM (1994) Anti-secretagogue anti-ulcer and cytoprotective properties of Acorus calamus in rats. Fitoterapia 65, 19–23.Google Scholar
Reeves, PG, Nielsen, FH, Fahey, GC Jr (1993) AIN-93 purified diets for laboratory rodents: report of the American Institute of Nutrition ad hoc working committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 1939–1951.CrossRefGoogle Scholar
Robert, A, Nezamis, JE, Lancaster, C & Hanchar, AJ (1979) Cytoprotection ny prostaglandins in rats. Prevention of gastric necroses produced by alcohol, HCl, NaOH, hypotonic and thermal injury. Gastroenterology 77, 761–767.CrossRefGoogle Scholar
Sant'ana, DMG, de Miranda-Neto, MH, de Souza, RR & Molinari, SL (1997) Morphological and quantitative study of the myenteric plexus of the ascending colon of rats subjected to proteic disnutrition. J Neuropsychiatr 55, 687–695.Google Scholar
Sertie, JA, Basile, AC, Panizza, S, Matida, AK & Zelnik, R (1990) Planta Med 56, 36–40.Google Scholar
Shay, H, Komarov, SA, Fels, SS, Meranze, D, Gruenstein, M & Siplet, H (1945) A simple method for the uniform production of gastric ulceration in rat. Gastroenterology 5, 43–61.Google Scholar
Stone, EA, Plott, J, Herrero, A & Kirk, LK (1986) Effect of repeated restraint stress, desmethylimipiramine or adrenocorticotropin on the alpha and beta adrenergic components of the cyclic AMP response to norepinephrine in rat brain slices. J Pharm Exp Ther 237, 702–707.Google ScholarPubMed
Sun, SB, Matsumoto, T & Yamada, H (1991) Histological study of mechanism of adaptive cytoprotection on ethanol-induced mucosal damage in rat stomachs. J Pharm Pharmacol 43, 699–704.Google Scholar
Szabo, S & Vinze, A (2000) Growth factors in ulcer healing: lessons from recent studies. J Physiol 94, 77–81.Google ScholarPubMed
Takagi, K, Okabe, S & Saziki, R (1969) A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing. Jpn J Pharmacol 19, 418–426.Google Scholar
Trevethick, MA, Oakley, I, Clayton, NM & Strong, P (1995) Review: Non-steroidal anti-inflammatory drug-induced gastric damage in experimental animals: underlying pathological mechanism. Gen Pharmacol 26, 1455–1459.CrossRefGoogle Scholar
Weaver, LT, Austin, S & Cole, TJ (1991) Small intestinal length: a factor essential for gut adaptation. Gut 32, 1321–1323.Google Scholar
Weaver, LT, Desai, M, Austin, S, Arthur, HM, Lucas, A & Hales, CN (1998) Effects of protein restriction in early life on growth and function of the gastrointestinal tract of the rat. J Pediatr Gastroenterol Nutr 27, 553–559.Google ScholarPubMed
Yago, MD, Serrano, P, Manãs, M, Mataix, J, Medrano, J, Calpena, R, Martinez-Victoria, E (1998) Release of secretin and somatostatin after test meals with different fatty-acid composition in cholecystectomized humans. J Nutr Biochem 9, 186–192.CrossRefGoogle Scholar
Young, CM, Lee, PC & Lebenthal, E (1987) Maternal dietary restriction during pregnancy and lactation: effect on digestive organ development in suckling rats. Am J Clin Nutr 46, 36–40.Google Scholar