Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-12-03T19:12:13.207Z Has data issue: false hasContentIssue false
  • English
  • Français

Iron absorption from experimental infant formulas based on pea (Pisum sativum)-protein isolate: the effect of phytic acid and ascorbic acid

Published online by Cambridge University Press:  09 March 2007

Lena Davidsson ,
Triantafillia Dimitriou ,
Thomas Walczyk  and
Richard F. Hurrell
Lena Davidsson*
Affiliation:
Laboratory for Human Nutrition, Institute of Food Science, Swiss Federal Institute of Technology (ETHZürich), PO Box 474, CH-8803 Rüschlikon, Switzerland
Triantafillia Dimitriou
Affiliation:
Laboratory for Human Nutrition, Institute of Food Science, Swiss Federal Institute of Technology (ETHZürich), PO Box 474, CH-8803 Rüschlikon, Switzerland
Thomas Walczyk
Affiliation:
Laboratory for Human Nutrition, Institute of Food Science, Swiss Federal Institute of Technology (ETHZürich), PO Box 474, CH-8803 Rüschlikon, Switzerland
Richard F. Hurrell
Affiliation:
Laboratory for Human Nutrition, Institute of Food Science, Swiss Federal Institute of Technology (ETHZürich), PO Box 474, CH-8803 Rüschlikon, Switzerland
*
*Corresponding author: Lena Davidsson, fax +41 1 704 5710 email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Infant formula based on pea (Pisum sativum)-protein isolate has been suggested as an alternative to soyabean formula in countries where soyabean is not a native crop, or when soyabean protein cannot be used due to allergic reactions or intolerances. In the present study, Fe absorption from experimental infant formulas based on pea-protein isolate was measured in healthy non-anaemic young women. The influence of phytic acid and ascorbic acid on Fe absorption was evaluated, using a stable-isotope technique based on incorporation of Fe stable-isotope labels into erythrocytes 14 d after administration. Geometric mean Fe absorption increased from 20·7 (+1SD 41·6, -1sd 10·3) % to 33·1 (+1sd 58·6, -1sd 18·7) %; (P<0·0001; n 10) after enzymic degradation of virtually all phytic acid. Doubling the molar ratio Fe : ascorbic acid from 1 : 2·1 to 1 : 4·2 in the infant formula with native phytic acid content also increased Fe absorption significantly (P<0·0001; n 10); geometric mean Fe absorption increased from 14·8 (+1sd 32·1, -1sd 6·8) % to 22·1 (+1sd 47·2, -1sd 10·4) %. These results confirm the inhibitory and enhancing effects of phytic acid and ascorbic acid respectively on Fe absorption, but also indicate relatively high fractional Fe absorption from the pea-protein-based formulas. After adjusting for differences in Fe status, our data indicate that Fe absorption from dephytinised pea protein might be less inhibitory than dephytinised soyabean protein as measured in a previous study ().

Keywords

Pea proteinPhytic acidAscorbic acidIronStable isotopes
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 1 , January 2001 , pp. 59 - 63
Copyright
Copyright © The Nutrition Society 2001

References

Beer, B & Heumann, KG (1993) Isotope dilution mass spectrometry of microelectronically relevant heavy metal traces in high purity cobalt. Fresenius Journal of Analytical Chemistry 347, 351–355.CrossRefGoogle Scholar
Brown, E, Hopper, J, Hodges, JL, Bradley, B, Wennesland, R & Yamauchi, H (1962) Red cell, plasma and blood volume in healthy women measured by radiochromium cell-labeling and hematocrit. Journal of Clinical Investigation 41, 2182–2190.CrossRefGoogle ScholarPubMed
Cook, JD, Dassenko, SA & Lynch, SR (1991) Assessment of the role of nonheme-iron bioavailability in iron balance. American Journal of Clinical Nutrition 54, 717–722.CrossRefGoogle ScholarPubMed
Cook, JD, Lipschitz, DA, Miles, LE & Finch, CA (1974) Serum ferritin as a measure of iron stores in normal subjects. American Journal of Clinical Nutrition 27, 681–687.CrossRefGoogle ScholarPubMed
Davidsson, L, Galan, P, Kastenmayer, P, Cherouvrier, F, Juillerat, M-A, Hercberg, S & Hurrell, RF (1994) Iron bioavailability studied in infants: The influence of phytic acid and ascorbic acid in infant formulas based on soy isolate. Pediatric Research 36, 816–822.CrossRefGoogle ScholarPubMed
Gillooly, M, Torrance, JD, Bothwell, TH, MacPhail, AP, Derman, D, Mills, W & Mayet, F (1984) The relative effect of ascorbic acid on iron absorption from soy-based and milk-based infant formulas. American Journal of Clinical Nutrition 40, 522–527.CrossRefGoogle ScholarPubMed
Golden, BE & Golden, MHN (1981) Plasma zinc, rate of weight gain, and the energy cost of tissue deposition in children recovering from severe malnutrition on a cow's milk or soya protein based diet. American Journal of Clinical Nutrition 34, 892–899.CrossRefGoogle ScholarPubMed
Guéguen, J, Lemarié, J & B, Godon (1996) Composition, structure et propriétés physicochimiques des protéines de légumineuses et d'oléagineux (Composition, structure and physicochemical properties of the proteins of legumes and oil-producing crops). In Protéines végétales (Vegetable Proteins), pp. 1–40. [B, Godon, editor]. Paris: Lavoisier.Google Scholar
Hallberg, L, Brune, M & Rossander, L (1989) Iron absorption in man: ascorbic acid and dose-dependent inhibition by phytate. American Journal of Clinical Nutrition 49, 140–144.CrossRefGoogle ScholarPubMed
Hurrell, RF, Davidsson, L, Reddy, M, Kastenmayer, P & Cook, JD (1998) A comparison of iron absorption in adults and infants consuming identical infant formulas. British Journal of Nutrition 79, 31–36.CrossRefGoogle ScholarPubMed
Hurrell, RF, Juillerat, M-A, Reddy, MB, Lynch, SR, Dassenko, SA & Cook, JD (1992) Soy protein, phytate, and iron absorption in humans. American Journal of Clinical Nutrition 56, 573–578.CrossRefGoogle ScholarPubMed
Kastenmayer, P, Davidsson, L, Galan, P, Cherouvrier, F, Hercberg, S & Hurrell, RF (1994) A double stable isotope technique for measuring iron absorption in infants. British Journal of Nutrition 71, 411–424.CrossRefGoogle ScholarPubMed
Lynch, SR, Dassenko, SA, Cook, JD, Juillerat, M-A & Hurrell, RF (1994) Inhibitory effect of a soy-bean protein-related moiety on Fe absorption in humans. American Journal of Clinical Nutrition 60, 567–572.CrossRefGoogle Scholar
Magnusson, B, Björn-Rasmussen, E, Hallberg, L & Rossander, L (1981) Iron absorption in relation to iron status. Model proposed to express results to food iron absorption measurements. Scandinavian Journal of Haematology 27, 201–208.CrossRefGoogle ScholarPubMed
Sandström, B, Cederblad, A & Lönnerdal, B (1983) Zinc absorption from human milk, cow's milk, and infant formulas. American Journal of Diseases of Children 137, 726–729.Google ScholarPubMed
Siegenberg, D, Baynes, RD, Bothwell, TH, Macfarlane, BJ, Lamparelli, RD, Car, NG, MacPhail, P, Schmidt, U, Tal, A & Mayet, F (1991) Ascorbic acid prevents the dose-dependent inhibitory effects of polyphenols and phytates on nonheme iron absorption. American Journal of Clinical Nutrition 53, 537–541.CrossRefGoogle ScholarPubMed
Skoglund, E, Carlsson, N-G & Sandberg, A-S (1997) Determination of isomers of inositol mono- to hexaphosphates in selected foods and intestinal contents using high-performance ion chromatography. Journal of Agricultural Food Chemistry 45, 431–436.CrossRefGoogle Scholar
Taylor, PDP, Maeck, R & De Bièvre, P (1992) Determination of the absolute isotopic composition and atomic weight of a reference sample of natural iron. International Journal of Mass Spectrometry and Ion Processes 121, 111–125.CrossRefGoogle Scholar
Walczyk, T (1997) Iron isotope ratio measurements by negative thermal ionization mass spectrometry using FeF4- ions. International Journal of Mass Spectrometry and Ion Processes 161, 217–221.CrossRefGoogle Scholar
Walczyk, T, Davidsson, L, Zavaleta, N & Hurrell, RF (1997) Stable isotope labels as a tool to determine iron absorption by Peruvian school children from a breakfast meal. Fresenius Journal of Analytical Chemistry 359, 445–449.CrossRefGoogle Scholar
Walters, GO, Jacobs, A, Worwood, M, Trevett, D & Thomson, W (1975) Iron absorption in normal subjects and patients with idiopathic haemochromatosis; relationship with serum ferritin concentrations. Gut 16, 188–192.CrossRefGoogle Scholar