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Ingestion of insoluble dietary fibre increased zinc and iron absorption and restored growth rate and zinc absorption suppressed by dietary phytate in rats

Published online by Cambridge University Press:  09 March 2007

Kumiko Hayashi ,
Hiroshi Hara ,
Patchana Asvarujanon ,
Yoritaka Aoyama  and
Pairojana Luangpituksa
Kumiko Hayashi
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Hiroshi Hara*
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Patchana Asvarujanon
Affiliation:
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
Yoritaka Aoyama
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Pairojana Luangpituksa
Affiliation:
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
*
*Corresponding author: Dr Hiroshi Hara, fax +81 11 706 2504 or +81 11 716 0879, email [email protected]
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Abstract

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We examined the effects of ingestion of five types of insoluble fibre on growth and Zn absorption in rats fed a marginally Zn-deficient diet (6·75 mg (0·103 mmol) Zn/kg diet) with or without added sodium phytate (12·6 mmol/kg diet). The types of insoluble fibre tested were corn husks, watermelon skin, yam-bean root (Pachyrhizus erosus) and pineapple core, and cellulose was used as a control (100 g/kg diet). Body-weight gain in the cellulose groups was suppressed by 57 % by feeding phytate. Body-weight gain in phytate-fed rats was 80 % greater in the watermelon skin fibre and yam-bean root fibre group than that in the cellulose group. Zn absorption ratio in the cellulose groups was lowered by 46 and 70 % in the first (days 7–10) and second (days 16–19) measurement periods with feeding phytate. In the rats fed the phytate-containing diets, Zn absorption ratio in the watermelon skin, yam-bean root and pineapple core fibre groups was 140, 80 and 54 % higher respectively than that in the cellulose group, in the second period. Fe absorption was not suppressed by phytate, however, feeding of these three types of fibre promoted Fe absorption in rats fed phytate-free diets. The concentration of soluble Zn in the caecal contents in the watermelon skin fibre or yam-bean root fibre groups was identical to that in the control group in spite of a higher short-chain fatty acid concentration and lower pH in the caecum. These findings indicate that ingestion of these types of insoluble fibre recovered the growth and Zn absorption suppressed by feeding a high level of phytate, and factors other than caecal fermentation may also be involved in this effect of insoluble fibre.

Keywords

Insoluble dietary fibreZinc absorptionPhytic acidCaecal fermentationBulking effectRats
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 4 , October 2001 , pp. 443 - 451
Copyright
Copyright © The Nutrition Society 2001

References

American Institute of Nutrition (1977) Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
American Institute of Nutrition (1980) Second report of the ad hoc committee on standards for nutritional studies. Journal of Nutrition 110, 1726.CrossRefGoogle Scholar
Association of Official Analytical Chemists (1997) Official Methods of Analysis of the Association of Official Analytical Chemists International, 16th ed., vol 2. Arlington, VA: Association of Official Analytical Chemists, Inc.Google Scholar
Bagheri, SM & Gueguen, L (1982) Effects of wheat bran on the metabolism of calcium-45 and zinc-65 in rats. Journal of Nutrition 112, 2047–2051.Google Scholar
Cousins, RJ (1996) Zinc. In Present Knowledge in Nutrition, 7th ed., pp. 293–306 [Brown, ML, editor]. Washington, DC: International Life Sciences Institute.Google Scholar
Donangelo, CM & Eggum, BO (1986) Comparative effects of wheat bran and barley husk on nutrient utilization in rats. 2. Zinc, calcium and phosphorus. British Journal of Nutrition 56, 269–280.CrossRefGoogle ScholarPubMed
Duncan, DB (1995) Multiple range and multiple F tests. Biometrics 11, 1–42.CrossRefGoogle Scholar
Fairweather-Tait, SJ & Wright, AJA (1990) The effects of sugar-beet fibre and wheat bran on iron and zinc absorption in rats. British Journal of Nutrition 64, 547–552.CrossRefGoogle ScholarPubMed
Ferguson, EL & Gibson, RS (1993) The zinc nutriture of preschool children living in two African countries. Journal of Nutrition 123, 1487–1496.CrossRefGoogle ScholarPubMed
Franz, KB, Kennedy, BM & Fellers, DA (1980) Relative bioavailability of zinc from selected cereals and legumes using rat growth. Journal of Nutrition 110, 2272–2283.CrossRefGoogle ScholarPubMed
Giugliano, R & Millward, DJ (1984) Growth and zinc homeostasis in the severely Zn-deficient rat. British Journal of Nutrition 52, 545–560.CrossRefGoogle ScholarPubMed
Hara, H, Konishi, A & Kasai, T (2000) Contribution of the cecum and colon to zinc absorption in rats. Journal of Nutrition 130, 83–89.CrossRefGoogle ScholarPubMed
Hara, H, Nagata, M, Ohta, A & Kasai, T (1996) Increases in calcium absorption with ingestion of soluble dietary fibre, guar-gum hydrolysate, depend on the caecum in partially nephrectomized and normal rats. British Journal of Nutrition 76, 773–784.CrossRefGoogle ScholarPubMed
Hara, H, Suzuki, T, Kasai, T, Aoyama, Y & Ohta, A (1999) Ingestion of guar gum hydrolysate, a soluble fiber, increases calcium absorption in totally gastrectomized rats. Journal of Nutrition 129, 39–45.CrossRefGoogle ScholarPubMed
Harper, AE (1959) Amino acid balance and imbalance. 1. Dietary level of protein and amino acid imbalance. Journal of Nutrition 68, 405–418.CrossRefGoogle Scholar
Hoshi, S, Sakata, T, Mikuni, K, Hashimoto, H & Kimura, S (1994) Galactosylsucrose and xylosylfrutoside alter digestive tract size and concentrations of cecal organic acids in rats fed diets containing cholesterol and cholic acid. Journal of Nutrition 124, 52–60.CrossRefGoogle ScholarPubMed
Jiang, KS (1986) Effects of dietary celluose and xylan on absorption and tissue contents of zinc and copper in rats. Journal of Nutrition 116, 999–1006.CrossRefGoogle Scholar
Johansen, HN & Bach Knudsen, KE (1994) Effects of wheat-flour and oat mill fractions on jejunal flow, starch degradation and absorption of glucose over an isolated loop of jejunum in pigs. British Journal of Nutrition 72, 299–313.CrossRefGoogle ScholarPubMed
Larsson, M, Rossander, HL, Sandstrom, B & Sandberg, AS (1996) Improved zinc and iron absorption from breakfast meals containing malted oats with reduced phytate content. British Journal of Nutrition 76, 677–688.CrossRefGoogle ScholarPubMed
Lin, HC, Zhao, XT, Chu, AW, Lin, YP & Wang, L (1997) Fiber-supplemented enteral formula slows intestinal transit by intensifying inhibitory feedback from the distal gut. American Journal of Clinical Nutrition 65, 1840–1844.CrossRefGoogle ScholarPubMed
Lopez, HW, Coudray, C, Bellanger, J, Younes, H, Demigne, C & Remesy, C (1998) Intestinal fermentation lessens the inhibitory effects of phytic acid on mineral utilization in rats. Journal of Nutrition 128, 1192–1198.CrossRefGoogle ScholarPubMed
MacDonald, RS (2000) The role of zinc in growth and cell proliferation. Journal of Nutrition 130, 1500S–1508S.CrossRefGoogle ScholarPubMed
Mason, PM, Judd, PA, Fairweather-Tait, SJ, Eagles, J & Minski, M (1990) The effect of moderately increased intakes of complex carbohydrates (cereals, vegetables and fruit) for 12 weeks on iron and zinc metabolism. British Journal of Nutrition 63, 597–911.CrossRefGoogle ScholarPubMed
Miyazawa, E, Iwabuchi, A & Yoshida, T (1996) Phytate breakdown and apparent absorption of phosphorus, calcium and magnesium in germfree and conventionalized rats. Nutrition Research 16, 603–613.CrossRefGoogle Scholar
Navert, B, Sandstrom, B & Cederblad, A (1985) Reduction of the phytate content of bran by leavening in bread and it effects on zinc absorption in man. British Journal of Nutrition 53, 47–53.Google ScholarPubMed
Ohta, A, Baba, S, Takizawa, T & Adachi, T (1994) Effects of fructooligosaccharides on the absorption of magnesium in the magnesium-deficient rat model. Journal of Nutritional Science and Vitaminology (Tokyo) 40, 171–180.CrossRefGoogle ScholarPubMed
Pallauf, J & Rimbach, G (1997) Nutritional significance of phytic acid and phytase. Archiv fur Tierernahrung 50, 301–319.CrossRefGoogle ScholarPubMed
Reeves, PG (1989) AIN-76 diet: should we change the formulation? Journal of Nutrition 119, 1081–1082.CrossRefGoogle ScholarPubMed
Sandberg, AS, Brune, M, Carlsson, NG, Hallberg, L, Skoglund, E & Rossander, HL (1999) Inositol phosphates with different numbers of phosphate groups influence iron absorption in humans. American Journal of Clinical Nutrition 70, 240–246.CrossRefGoogle ScholarPubMed
Sandberg, AS, Hasselblad, C, Hasselblad, K & Hulten, L (1982) The effect of wheat bran on the absorption of minerals in the small intestine. British Journal of Nutrition 48, 185–191.CrossRefGoogle ScholarPubMed
Sandstrom, B, Bugel, S, McGraw, BA, Price, J & Reid, MD (2000) A high oat-bran intake does not impair zinc absorption in humans when added to a low-fiber animal protein-based diet. Journal of Nutrition 130, 594–599.CrossRefGoogle Scholar
Takeda, H & Kiriyama, S (1979) Correlation between the physical properties of dietary fibers and their protective activity against amaranth toxicty in rats. Journal of Nutrition 109, 388–396.CrossRefGoogle Scholar
Thebaudin, JY, Lefebvre, AC, Harrington, M & Bourgeois, CM (1997) Dietary fibres: Nutritional and technological interest. Trends in Food Science and Technology 8, 41–47.CrossRefGoogle Scholar
Trinidad, TP, Wolever, TM & Thompson, LU (1996) Effect of acetate and propionate on calcium absorption from the rectum and distal colon of humans. American Journal of Clinical Nutrition 63, 574–578.CrossRefGoogle ScholarPubMed
Tidehag, P, Hallmans, G, Wing, K, Sjostrom, R, Agren, G, Lundin, E & Zhang, J (1996) A comparison of iron absorption from single meals and daily diets using radio Fe (55Fe, 59Fe). British Journal of Nutrition 75, 281–289.Google Scholar
Wise, A & Gilburt, DJ (1982) Phytate hydrolysis by germfree and conventional rats. Applied and Environmental Microbiology 43, 753–756.CrossRefGoogle ScholarPubMed
Younes, H, Demigne, C & Remesy, C (1996) Acidic fermentation in the caecum increases absorption of calcium and magnesium in the large intestine of the rat. British Journal of Nutrition 75, 301–314.CrossRefGoogle ScholarPubMed
Zhou, JR, Fordyce, EJ, Raboy, V, Dickinson, DB, Wong, MS, Burns, RA & Erdman, JJ (1992) Reduction of phytic acid in soybean products improves zinc bioavailability in rats. Journal of Nutrition 122, 2466–2473.CrossRefGoogle ScholarPubMed