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Influence of oat saponins on intestinal permeability in vitro and in vivo in the rat

Published online by Cambridge University Press:  09 March 2007

Gunilla Önning ,
Quan Wang ,
Björn R Weström ,
Nils-Georg Asp  and
Börje W Karlsson
Gunilla Önning
Affiliation:
Department of Applied Nutrition and Food Chemistry, Chemical Center, Lund University, PO Box 124, 4221 00, Lund, Sweden
Quan Wang
Affiliation:
Department of Animal Physiology, Lund University, S-223 62, Lund, Sweden
Björn R Weström
Affiliation:
Department of Animal Physiology, Lund University, S-223 62, Lund, Sweden
Nils-Georg Asp
Affiliation:
Department of Applied Nutrition and Food Chemistry, Chemical Center, Lund University, PO Box 124, 4221 00, Lund, Sweden
Börje W Karlsson
Affiliation:
Department of Animal Physiology, Lund University, S-223 62, Lund, Sweden
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Abstract

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The aim of the present study was to investigate whether oat saponins (avenacosides A and B) have any effect on the permeability of the rat intestine to actively and passively transported markers in vitro and in vivo. Intestinal segments were mounted in modified Ussing chambers, and thepassage of the different marker compounds from the mucosal to the serosal side was measured for 120 min. Avenacosides (1 mg/ml) gave a significantly higher passage of the macromolecule ovalbumin and there was a tendency to increased passage of [14C]D-mannitol and [15Cr]EDTA. On the other hand, the saponins did not affect the active transport of [3H]methyl glucose. When rats were given saponins (40 mg/kg body weight)together with markers by gastric intubation, the passage of [51Cr]EDTA into blood and urine was somewhat reduced. For the macromolecule bovine serum albumin, no evident effect on the passage was observed in the presence ofsaponins. Thus, in contrast to the in vitro results, the in vivo marker passage seemed to be unaffected or even reduced in the presence of avenacosides. The study shows that saponinscan affect the permeability of the rat intestine. However, this effect needs further investigation in vivo, especially regarding proteins.

Keywords

SaponinsOatsIntestinal permeability
Type
General Nutrition
Information
British Journal of Nutrition , Volume 76 , Issue 1 , July 1996 , pp. 141 - 151
Copyright
Copyright © The Nutrition Society 1996

References

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