Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-25T02:42:54.207Z Has data issue: false hasContentIssue false
  • English
  • Français

β-Galactosidase production by Streptococcus thermophilus is higher in the small intestine than in the caecum of human-microbiota-associated mice after lactose supplementation

Published online by Cambridge University Press:  08 March 2007

Denis D.G. Mater ,
Sophie Drouault-Holowacz ,
Raish Oozeer ,
Philippe Langella ,
Jamila Anba  and
Gérard Corthier
Denis D.G. Mater
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Sophie Drouault-Holowacz
Affiliation:
Arilait Recherches, 42 rue de Châteaudun, 75009 Paris, France
Raish Oozeer
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Philippe Langella
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Jamila Anba
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Gérard Corthier*
Affiliation:
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
*
*Corresponding author Dr Gérard Corthier, fax +33 134 652462, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Transit kinetics and survival rates of a bacterial species from yoghurt (i.e. Streptococcus thermophilus strain FBI3) were examined in different digestive compartments of gnotoxenic and human-microbiota-associated mice. The production of the lactose-hydrolysing enzyme (i.e. β-galactosidase) was also investigated within the digestive tract, using a chromosomal reporter system based on luciferase genes from Photorhabdus luminescens under the control of the plac promoter. In both mice models, S. thermophilus cells transited within 2h from the stomach to the caecum–colon compartment of the digestive tract where they displayed a survival rate of nearly 100%. In gnotoxenic mice, luciferase activity was found to increase in the second half of the small intestine and in the caecum–colon compartment when lactose was added to the drinking water provided to the animals. In human-microbiota-associated mice drinking lactose, luciferase activity was similarly increased in the second half of the small intestine but was drastically reduced in the caecum–colon compartment. This feature could be ascribed to the presence of the resident human microbiota.

Keywords

Digestive tractHuman microbiotaLactose intoleranceStreptococcus thermophilusYoghurt
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 177 - 181
Copyright
Copyright © The Nutrition Society 2006

References

Andremont, A, Raibaud, P, Tancrede, C, Duval-Iflah, Y, Ducluzeau, RThe use of germ-free mice associated with human fecal flora as an animal model to study enteric bacterial interactions. InBacterial Diarrheal Diseases PP. Takeda, Y, Miwatani, TTokyo: KTK Scientific Publishers. 1985 219–228.Google Scholar
Bayless, TM, Huang, SS, Recurrent abdominal pain due to milk and lactose intolerance in school-aged children. Pediatrics (1971) 47 1029–1032.CrossRefGoogle ScholarPubMed
Corthier, G, Delorme, C, Ehrlich, SD, Renault, P, Use of luciferase genes as biosensors to study bacterial physiology in the digestive tract.. Appl Environ Microbiol (1998) 64 2721–2722.Google Scholar
de Vrese, M, Stegelmann, A, Richter, B, Fenselau, S, Laue, C, Schrezenmeir, J, Probiotics – compensation for lactase insuf-ficiency. Am J Clin Nutr (2001) 73 Suppl 2421S–429S.Google Scholar
Drouault, s, Anba, J, Corthier, G, Streptococcus thermophilus is able to produce a beta-galactosidase active during its transit in the digestive tract of germ-free mice. Appl Environ Microbiol (2002) 68 938–941.Google Scholar
Ducluzeau, R, Bellier, M, Raibaud, P, Transit through the digestive tract of the inocula of several bacterial strains introduced “per os” into axenic and “holoxenic” mice. The antagonistic effect of the microflora of the gastrointestinal tract. Zentralbl Bakteriol (1970) 213 533–548.Google Scholar
Hirayama, K, Ex-germfree mice harboring intestinal microbiota derived from other animal species as an experimental model for ecology and metabolism of intestinal bacteria. Exp Anim (1999) 48 219–227.CrossRefGoogle ScholarPubMed
Lerebours, E, N'Djitoyap Ndam, C, Lavoine, A, Hellot, MF, Antoine, JM, Colin, R, Yogurt and fermented-then-pasteurized milk: effects of short-term and long-term ingestion on lactose absorption and mucosal lactase activity in lactase-deficient subjects. Am J Clin Nutr (1989) 49 823–827.Google Scholar
Marteau, P, Flourie, B, Pochart, P, Chastang, C, Desjeux, JF, Rambaud, JC, Effect of the microbial lactase (EC 3.2.1.23)activity in yoghurt on the intestinal absorption of lactose: an in vivo study in lactase-deficient humans. Br J Nutr (1990) 64 71–79.CrossRefGoogle Scholar
Mater, DDG, Bretigny, L, Firmesse, O, Flores, M-J, Mogenet, A, Bresson, J-L, Corthier, G, Streptococcus thermophilus and Lactobacillus delbrueckiisubsp. bulgaricus survive gastrointestinal transit of healthy volunteers consuming yogurt. FEMS Microbiol Lett (2005) 250 185–187.CrossRefGoogle Scholar
Meighen, EA, Molecular biology of bacterial bioluminescence. Microbiol Rev (1991) 55 123–142.CrossRefGoogle ScholarPubMed
Oozeer, R, Goupil-Feuillerat, N, Alpert, CA, van de Guchte, M, Anba, J, Mengaud, J, Corthier, G, Lactobacillus casei is able to survive and initiate protein synthesis during its transit in the digestive tract of human flora associated mice. Appl Environ Microbiol (2002) 68 3570–3574.CrossRefGoogle ScholarPubMed
Oozeer, R, Mater, DDG, Goupil-Feuillerat, N, Corthier, G, Initiation of protein synthesis by a labeled derivative of theLactobacillus casei DN-114 001 strain during transit from the stomach to the cecum in mice harboring human microbiota. Appl Environ Microbiol (2004) 70 6992–6997.Google Scholar
Rizkalla, SW, Luo, J, Kabir, M, Chevalier, A, Pacher, N, Slama, GChronic consumption of fresh but not heated yogurt improves breath-hydrogen status and short-chain fatty acid profiles:a controlled study in healthy men with or without lactose maldigestion. Am J Clin Nutr (2000) 72 1474–1479.Google Scholar
Savaiano, DA, Abou El Anouar, A, Smith, DE, Levitt, MD, Lactose malabsorption from yogurt, pasteurized yogurt, sweet acidophilus milk, and cultured milk in lactase-deficient individuals. Am J Clin Nutr (1995) 40 1219–1223.CrossRefGoogle Scholar
Shermak, MA, Saavedra, JM, Jackson, TL, Huang, SS, Bayless, TM, Perman, JA, Effect of yogurt on symptoms and kinetics of hydrogen production in lactose-malabsorbing children. Am J Clin Nutr (1995) 62 1003–1006.Google Scholar
Simoons, FJ, The geographic hypothesis and lactose malabsorption. A weighting of evidence. Am J Dig Dis (1978) 23 963–980.CrossRefGoogle Scholar
Varela-Moreiras, G, Antoine, JM, Ruiz-Roso, B, Varela, G, Effects of yogurt and fermented-then-pasteurized milk on lactose absorption in an institutionalized elderly group. J Am Coll Nutr (1992) 11 168–171.Google Scholar