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Enhanced bioavailability of zeaxanthin in a milk-based formulation of wolfberry (Gou Qi Zi;Fructus barbarum L.)

Published online by Cambridge University Press:  08 March 2007

Iris F. F. Benzie*
Affiliation:
Department of Health Technology and Informatics, The Hong Kong Polytechnic UniversityKowloon, Hong Kong
Wai Y. Chung
Affiliation:
Department of Health Technology and Informatics, The Hong Kong Polytechnic UniversityKowloon, Hong Kong
Junkuan Wang
Affiliation:
Nestlé Research CenterLausanne, Switzerland
Myriam Richelle
Affiliation:
Nestlé Research CenterLausanne, Switzerland
Peter Bucheli
Affiliation:
Nestlé R&D CenterShanghai Ltd, Shanghai, China
*
*Corresponding author Professor Iris Benzie, fax + 852 23624365, email [email protected]
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Abstract

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The carotenoid zeaxanthin is concentrated within the macula. Increased macular zeaxanthin is suggested to lower the risk of age-related macular degeneration. The small red berry, wolfberry (Fructus barbarum L.; Gou Qi Zi and Kei Tze), is one of the richest natural sources of zeaxanthin. However, carotenoid bioavailability is low, and food-based products with enhanced bioavailability are of interest. The present study investigated zeaxanthin bioavailability from three wolfberry formulations. Berries were homogenised in hot (80°C) water, warm (40°C) skimmed milk and hot (80°C) skimmed milk, with freeze drying of each preparation into a powdered form. A zeaxanthin-standardised dose (15mg) of each was consumed, in randomised order, together with a standardised breakfast by twelve healthy, consenting subjects in a cross-over trial, with a 3–5-week washout period between treatments. Blood samples were taken via a venous cannula immediately before (fasting) and 2, 4, 6, 7, 8 and 10h post-ingestion. Zeaxanthin concentration in the triacylglycerol-rich lipoprotein fraction of plasma was measured by HPLC. Results showed that triacylglycerol-rich lipoprotein zeaxanthin peaked at 6h post-ingestion for all formulations. Zeaxanthin bioavailability from the hot milk formulation was significantly higher (p<0·001) than from the others. Mean area under the curve (n 12) results were 9·73 (sem 2·45), 3·24 (sem 0·72) and 3·14 (sem 1·09) nmol×h/l for the hot milk, warm milk and hot water formulations, respectively. Results showed clearly that homogenisation of wolfberry in hot skimmed milk results in a formulation that has a 3-fold enhanced bioavailability of zeaxanthin compared with both the ‘classical’ hot water and warm skimmed milk treatment of the berries.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2006

References

Aebischer, CP, Schierle, J & Schüep, WSimultaneous determination of retinol, tocopherols, carotene, lycopene, and xanthophylls in plasma by means of reversed-phase high-performance liquid chromatography. Methods Enzymol (1999) 299 348362.CrossRefGoogle ScholarPubMed
Bai, SResearch on Ningxia Wolfberry(Lycium barbarum), vols. 1 and 2. China: People's Publishing House 1999Google Scholar
Bartlett, H & Eperjesi, FAge-related macular degeneration and nutritional supplementation: a review of randomized controlled trials. Ophthal Physiol Opt (2003) 23 383399.CrossRefGoogle Scholar
Beatty, S, Boulton, M, Henson, D, Koh, HH & Murray, IJMacular pigment and age related macular degeneration. Br J Ophthalmol (1999) 83 867877.CrossRefGoogle ScholarPubMed
Beatty, S, Koh, H, Henson, D & Boulton, MThe role of oxidative stress in the pathogenesis of age-related macular. Surv Ophthalmol (2000) 45 115134.CrossRefGoogle ScholarPubMed
Benzie, IFFEvolution of dietary antioxidants. J Compar Biochem Physiol (2003) 136 113126.CrossRefGoogle ScholarPubMed
Bone, RA, Landrum, JT, Dixon, Z, Chen, Y & Llerena, CMLutein and zeaxanthin in the eyes, serum and diet of human subjects. Exp Eye Res (2000) 71 239245.CrossRefGoogle ScholarPubMed
Bone, RA, Landrum, JT, Guerra, LH & Ruiz, CALutein and zeaxanthin dietary supplements raise macular pigment density and serum concentrations of these carotenoids in humans. J Nutr (2003) 133 992998.CrossRefGoogle ScholarPubMed
Breithaupt, DE, Weller, P, Wolters, M & Hahn, AComparison of plasma responses in human subjects after the ingestion of 3R,3R0-zeaxanthin dipalmitate from wolfberry (Lycium barbarum) and non-esterified 3R,3R0-zeaxanthin using chiral high-performance liquid chromatography. Br J Nutr (2004) 91 707713.CrossRefGoogle ScholarPubMed
Cheng, CY, Chung, WY, Szeto, YT & Benzie, IFFFasting plasma zeaxanthin response to Fructus barbarum L (wolfberry; Kei Tze) in a food-based human supplementation trial. Br J Nutr (2005) 93 123130.CrossRefGoogle Scholar
Curcio, CA, Owsley, C & Jackson, GRSpare the rods, save the cones in aging and age-related maculopathy. Invest Ophthalmol Vis Sci (2000) 41 20152018.Google ScholarPubMed
Dwyer, JH, Paul-Labrador, MJ, Fan, J, Shircore, AM, Merz, CNB & Dwyer, KMProgression of carotid intima-media thickness and plasma antioxidants: The Los Angeles Atherosclerosis Study. Arterioscler Thromb Vasc Biol (2004) 24 313319.CrossRefGoogle ScholarPubMed
Gonzalez, S, Astner, S, An, W, Goukassian, D & Pathak, MADietary lutein/zeaxanthin decreases ultraviolet B-induced epidermal hyperproliferation and acute inflammation in hairless mice. J Invest Dermatol (2003) 121 399405.CrossRefGoogle ScholarPubMed
Hammond, BR Jr, Johnson, EJ, Russell, RM, Krinsky, NI, Yeum, KJ, Edwards, RB & Snodderly, DMDietary modification of human macular pigment. Invest Opthalmol Vis Sci (1997) 38 17951801.Google ScholarPubMed
Handelman, GJ, Dratz, EA, Reay, CC & van Kuijk, JGCarotenoids in the human macula and whole retina. Invest Ophthalmol Vis Sci (1988) 29 850855.Google ScholarPubMed
Handlelman, GJ, Nightingale, ZD, Lichenstein, AH, Scaefer, EJ & Blumberg, JBLutein and zeaxanthin in plasma after dietary supplementation with egg yolk. Am J Clin Nutr (1999) 70 247251.CrossRefGoogle Scholar
Hartmann, D, Thurmann, PA, Spitzer, V, Schalch, W, Manner, B & Cohn, WPlasma kinetics of zeaxanthin and 30-dehydrolutein after multiple oral doses of synthetic zeaxanthin. Am J Clin Nutr (2004) 79 410417.CrossRefGoogle ScholarPubMed
Hubacek, JA, Berge, KE, Stefkova, J, Pitha, J, Skodova, Z, Lanska, V & Poledne, RPolymorphisms in ABCG5 and ABCG8 transporters and plasma cholesterol levels. Physiol Res (2004) 53 395401.CrossRefGoogle ScholarPubMed
Humphries, JA & Khachik, FDistribution of lutein, zeaxanthin, and related geometrical isomers in fruit, vegetables, wheat and pasta products. J Agric Food Chem (2003) 51 13221327.CrossRefGoogle ScholarPubMed
Hyman, L & Neborsky, RRisk factors for age-related macular degeneration: an update. Curr Opin Ophthalmol (2002) 13 171175.CrossRefGoogle ScholarPubMed
Krinsky, NI, Landrum, JT & Bone, RABiologic mechanisms of the protective role of lutein and zeaxanthin in the eye. Ann Rev Nutr (2003) 23 171201.CrossRefGoogle ScholarPubMed
Lam, KW & But, PThe content of zeaxanthin in Gou Qi Zi, a potential health benefit to improve visual acuity. Food Chem (1999) 67 173176.CrossRefGoogle Scholar
Landrum, JT & Bone, RALutein, zeaxanthin, and the macular pigment. Arch Biochem Biophys (2001) 385 2840.CrossRefGoogle ScholarPubMed
Landrum, JT, Bone, RA, Joa, H, Joa, H, Kilburn, MD, Moore, LL & Sprague, KEA one year study of the macular pigment: the effect of 140 days of a lutein supplement. Exp Eye Res (1997) 65 5762.CrossRefGoogle ScholarPubMed
Landrum, JT, Bone, RA, Moore, LL & Gomez, CMAnalysis of zeaxanthin distribution within individual human retinas. Methods Enzymol (1999) 299 457467.CrossRefGoogle ScholarPubMed
Le Marchand, L, Hankin, JH, Kolonel, LN, Beecher, GR, Wilkens, LR & Zhao, LPIntake of specific carotenoids and lung cancer risk. Cancer Epidemiol Biomarkers Prev (1993) 2 183187.Google ScholarPubMed
Leung, IYF, Tso, MOM, Li, WWY & Lam, TTAbsorption and tissue distribution of zeaxanthin and lutein in rhesus monkeys after taking Fructus lycii (Gou Qi Zi) extract. Invest Ophthalmol Vis Sci (2001) 42 466471.Google ScholarPubMed
Mares-Perlman, JA, Millen, AE, Ficek, TL & Hankinson, SEThe body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease: an overview. J Nutr (2002) 132 518S524S.CrossRefGoogle Scholar
Miwa, K, Inazu, A, Kobayashi, J, Higashikata, T,Nohara, A, Kawashiri, M, Katsuda, S, Takata, M, Koizumi, J & Mabuchi, HATPbinding cassette transporter G8 M429V polymorphism as a novel genetic marker of higher cholesterol absorption in hypercholesterolaemic Japanese subjects. Clin Sci (2005) 109 183188.CrossRefGoogle ScholarPubMed
Moeller, SM, Jacques, PF & Blumberg, JBThe potential role of dietary xanthophylls in cataract and age-related macular degeneration. J Am Coll Nutr (2000) 19, Suppl.5, 522S527S.CrossRefGoogle ScholarPubMed
Mozaffarieh, M, Sacu, S & Wedrich, AThe role of the carotenoids, lutein and zeaxanthin, in protecting against age-related macular degeneration: a review based on controversial evidence. Nutr J (2003) 2 2031.CrossRefGoogle ScholarPubMed
Olmedilla, B, Granado, F, Bianco, I, Vaquere, M & Cajigal, CLutein in patients with cataracts and age-related macular degeneration: a long-term supplementation study. J Sci Food Agric (2001) 81 904909.CrossRefGoogle Scholar
Parker, RS, Swanson, JE, You, CS, Edwards, AJ & Huang, TBioavailability of carotenoid in human subjects. Proc Nutr Soc (1999) 58 155162.CrossRefGoogle ScholarPubMed
Pérez-Gálvez, A, Martin, HD, Sies, H & Stahl, WIncorporation of carotenoids from paprika oleoresin into human chylomicrons. Br J Nutr (2003) 89 787793.CrossRefGoogle ScholarPubMed
Polidori, MC, Cherubini, A, Stahl, W, Senin, U, Sies, H & Mecocci, PPlasma carotenoid and malondialdehyde levels in ischemic stroke patients: relationship to early outcome. Free Radic Res (2002) 36 265268.CrossRefGoogle ScholarPubMed
Puddu, P, Cravero, E, Puddu, GM & Muscari, AGenes and atherosclerosis: at the origin of the predisposition. Int J Clin Pract (2005) 59 462472.CrossRefGoogle ScholarPubMed
Reboul, E, Abou, L, Mikail, C, Gghiringhelli, O, Andre, M,Portugal, H, Jourdheuil-Rahmani, D, Amiot, MJ, Lairou, D & Borel, PLutein transport by Caco-2 TC-7 cells occurs partly by a facilitated process involving the scavenger receptor class B type I (SR-BI). Biochem J (2005) 387 455461.CrossRefGoogle ScholarPubMed
Richelle, M, Bortlik, K, Liardet, S, Hager, C, Lambelet, P, Baur, M, Applegate, LA & Offord, EAA food-based formulation provides lycopene with the same bioavailability to humans as that from tomato paste. J Nutr (2002) 132 404408.CrossRefGoogle ScholarPubMed
Rose, RC, Richer, SP & Bode, AMOcular oxidants and antioxidant protection. PSEBM (1998) 217 397407.Google ScholarPubMed
Slattery, ML, Benson, J, Curtin, K, Ma, K, Shaeffer, D & Potte, RJDCarotenoids and colon cancer. Am J Clin Nutr (2000) 71 575582.CrossRefGoogle ScholarPubMed
Sommerburg, O, Keunen, JE, Bird, AC & van Kuijk, FJFruits and vegetables that are sources for lutein and zeaxanthin: the macular pigment in human eyes. Br J Ophthalmol (1998) 82 907910.CrossRefGoogle ScholarPubMed
Stokkermans, TJWTreatment of age-related macular degeneration. Clin Eye Vis Care (2000) 12 1535.CrossRefGoogle ScholarPubMed
Terpstra, AHMIsolation of serum chylomicrons prior to density gradient ultracentrifugation of other serum lipoprotein classes. Anal Biochem (1985) 150 221227.CrossRefGoogle ScholarPubMed
van het Hof, KH, West, CE, Weststrate, JA & Hautvast, JGAJDietary factors that affect the bioavailability of carotenoids. J Nutr (2000) 130 503506.CrossRefGoogle ScholarPubMed
Verschuren, WM, Jacobs, DR, Bloemberg, BP et al. ,. Serum total cholesterol and long term coronary heart disease mortality in different cultures. Twenty-five-year follow-up of the Seven Countries Study. JAMA (1995) 274 131136.CrossRefGoogle ScholarPubMed
Weller, P & Breithaupt, DEIdentification and quatification of zeaxanthin esters in plants using liquid chromatography-mass spectrometry. J Agric Food Chem (2003) 51 70447049.CrossRefGoogle Scholar
Woll, PS, Hanson, NQ, Arends, VL & Tsai, MYEffect of two common polymorphisms in the ATP binding cassette transporter A1 gene on HDL-cholesterol concentration. Clin Chem (2005) 51 907909.CrossRefGoogle ScholarPubMed
Zhou, L, Leung, I, Tso, MOM & Lam, KWThe identification of dipalmityl zeaxanthin as the major carotenoid in Gou Qi Zi by high pressure liquid chromatography and mass spectrometry. J Ocul Pharmacol Ther (1999) 15 557565.CrossRefGoogle ScholarPubMed