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Effects of the bacterial status of rats on the changes in some liver cytochrome P450 (EC 1.14.14.1) apoproteins consequent to a glucosinolate-rich diet

Published online by Cambridge University Press:  09 March 2007

L. Nugon-Baudon ,
S. Rabot ,
J.-P. Flinois ,
S. Lory  and
Ph. Beaune
L. Nugon-Baudon*
Affiliation:
INRA, Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josas Cedex, France
S. Rabot
Affiliation:
INRA, Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josas Cedex, France
J.-P. Flinois
Affiliation:
INSERM, U75, Centre Hospitalier Universitaire Necker, 156 rue de Vaugirard, F-75730 Paris Cedex 15, France
S. Lory
Affiliation:
INRA, Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josas Cedex, France
Ph. Beaune
Affiliation:
INSERM, U75, Centre Hospitalier Universitaire Necker, 156 rue de Vaugirard, F-75730 Paris Cedex 15, France
*
*Corresponding author: Dr Lionelle Nugon-Baudon, fax +33 1 34 65 24 62.
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Abstract

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The aim of the present work was to investigate the influence of the intestinal microflora on the changes in hepatic cytochrome P450 apoproteins induced by dietary glucosinolates. Ten rats harbouring a conventional digestive microflora were offered either a diet containing 390g myrosinase-free rapeseed meal/kg (n 5) or a control diet devoid of glucosinolates (n 5). A similar trial was performed using germ-free rats. After 4 weeks of exposure to the dietary regimens, animals were slaughtered and their livers removed for preparation of microsomes and analysis of cytochrome P450 (EC 1.14.14.1). The glucosinolate-rich diet decreased the concentration of total cytochrome P450 in conventional rats only (-34%). The bacterial status did not modify the concentration of apoproteins CYP1A2 and CYP2B1/B2, but greatly decreased the concentration of the male constitutive isoform CYP2C11 (-53 and -45% respectively in conventional and germ-free rats). Germ-free rats fed on the glucosinolate-rich diet had a greater concentration of CYP3A (+139%) and a lower concentration of CYP2E1 (-32%) than their counterparts fed on the control diet. However, these differences were absent in conventional animals. On the whole, the influence of the intestinal microflora on the changes in hepatic cytochrome P450 due to the consumption of cruciferous vegetables is very complex and obviously involves different mechanisms according to the apoprotein.

Keywords

GnotobiologyXenobiotic metabolizing enzymesRapeseed
Type
Short communication
Information
British Journal of Nutrition , Volume 80 , Issue 3 , September 1998 , pp. 231 - 234
Copyright
Copyright © The Nutrition Society 1998

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