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Effects of some poorly digestible carbohydrates on bile acid bacterial transformations in the rat

Published online by Cambridge University Press:  09 March 2007

Claude Andrieux ,
Daniele Gadelle ,
Christine Leprince  and
E. Sacquet
Claude Andrieux
Affiliation:
Laboratorie d'Ecologie Microbienne, INRA, Centre de Recherche de Jouy 78350, Jouy en Josas, France
Daniele Gadelle
Affiliation:
Laboratorie d'Ecologie Microbienne, INRA, Centre de Recherche de Jouy 78350, Jouy en Josas, France
Christine Leprince
Affiliation:
Laboratorie d'Ecologie Microbienne, INRA, Centre de Recherche de Jouy 78350, Jouy en Josas, France
E. Sacquet
Affiliation:
Laboratorie d'Ecologie Microbienne, INRA, Centre de Recherche de Jouy 78350, Jouy en Josas, France
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Abstract

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The effects of ingestion of poorly digestible carbohydrates on bacterial transformations of cholic acid and β-muricholic acid were studied in rats fed on increasing levels of lactose, lactulose, amylomaize or potato starches. Each level was given for 3 weeks and, at the end of each dietary treatment, bile acid faecal composition was analysed and a group of six rats was killed every 4 h during 24 h to determine the amounts of fermented carbohydrate and fermentation characteristics (caecal pH, volatile fatty acids (VFA) and lactic acid concentrations). Fermentation of carbohydrates decreased caecal pH and enhanced caecal VFA and lactic acid concentrations. Irrespective of the poorly digestible carbohydrate, the variation of bacterial transformation always occurred in the same way: the bacterial transformation of β-muricholic acid into hyodeoxycholic acid was the first to disappear, while ω-muricholic acid formation increased; second, cholic acid transformation decreased and finally all bile acid transformations were strongly affected. There was a significant correlation between bile acid transfer and the minimal caecal pH in vivo. This effect of pH was similar in vitro. To determine whether the levels of bacteria which transformed bile acids were modified, rats fed on the highest amounts of poorly digestible carbohydrates were introduced into isolators and carbohydrate feeding was stopped. Caecal pH recovered its initial value but bile acid transformations remained changed, suggesting that the intestinal microflora were modified by ingestion of fermentable carbohydrates.

Keywords

Bacterial transformationBile acidsCarbohydratesRat
Type
Research Article
Information
British Journal of Nutrition , Volume 62 , Issue 1 , July 1989 , pp. 103 - 119
Copyright
Copyright © The Nutrition Society 1989

References

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