Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-27T13:47:47.883Z Has data issue: false hasContentIssue false
  • English
  • Français

The effects of dietary tryptophan levels on growth and metabolism of rainbow trout (Salmo gairdneri)

Published online by Cambridge University Press:  09 March 2007

M. J. Walton ,
R. M. Coloso ,
C. B. Cowey ,
J. W. Adron  and
D. Knox
M. J. Walton
Affiliation:
NERC Institute of Marine Biochemistry, St Fittick's Road, Aberdeen AB1 3RA
R. M. Coloso
Affiliation:
NERC Institute of Marine Biochemistry, St Fittick's Road, Aberdeen AB1 3RA
C. B. Cowey
Affiliation:
NERC Institute of Marine Biochemistry, St Fittick's Road, Aberdeen AB1 3RA
J. W. Adron
Affiliation:
NERC Institute of Marine Biochemistry, St Fittick's Road, Aberdeen AB1 3RA
D. Knox
Affiliation:
NERC Institute of Marine Biochemistry, St Fittick's Road, Aberdeen AB1 3RA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Groups of rainbow trout (Salmo gairdneri) (mean weight 14 g) were given diets containing 0.8, 1.3, 2, 3, 4 or 6 g tryptophan/kg diet for 12 weeks.

2. By analysis of the growth results, the dietary requirement of tryptophan was found to be 2.5 g/kg diet (equivalent to 50 mg/kg biomass per d).

3. Carbon dioxide expired by trout following intraperitoneal injection of [14COOH]tryptophan contained little radioactivity when dietary tryptophan level was low but, above 2.0 g/kg diet, it increased rapidly with increasing dietary tryptophan level. The break point in the dose-response curve did not, however, coincide with that from the growth results.

4. Changes in concentrations of free tryptophan in blood and liver and activity of hepatic tryptophan pyrrolase (EC 1.13.11.11) in response to changes in dietary tryptophan concentration did not provide reliable indicators for quantifying dietary requirement. Unlike the situation in mammals, blood tryptophan was not protein-bound to any appreciable extent. Tryptophan pyrrolase of trout has properties which suggest it has no apoenzyme form.

5. In fish given adequate levels of tryptophan injected intraperitoneally with a tracer dose of [14COOH]tryptophan, 60% of the dose was incorporated into body protein within 1 d. The turnover of the label in this protein is very slow.

6. Those trout given diets deficient in tryptophan suffered from severe scoliosis and lordosis as well as having increased liver and kidney levels of calcium, magnesium, sodium and potassium.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 51 , Issue 2 , March 1984 , pp. 279 - 287
Copyright
Copyright © The Nutrition Society 1984

References

Badaway, A. A. B. & Evans, M. (1975). Biochemical Journal 150, 511–520.CrossRefGoogle Scholar
Badaway, A. A. B. & Evans, M. (1976). Biochemical Journal 158, 79–88.CrossRefGoogle Scholar
Barash, H., Poston, H. A. & Rumsey, G. L. (1982). Cornell Veterinarian 72, 361–371.Google Scholar
Basha, S. M. M. & Roberts, R. M. (1977). Analytical Biochemistry 77, 378–386.CrossRefGoogle Scholar
Bender, D. A. (1978). Amino Acid Metabolism. Chichester: John Wiley & Sons.Google Scholar
Bloxam, D. L. & Warren, W. H. (1974). Analytical Biochemistry 60, 621–625.CrossRefGoogle Scholar
Brown, J. N. & Dodgen, C. L. (1968). Biochimica Biophysica Acta 165, 463–469.CrossRefGoogle Scholar
Cowey, C. B., Adron, J. W., Walton, M. J., Murray, J., Youngson, A. & Knox, D. (1981). Journal of Nutrition 111, 1556–1567.CrossRefGoogle Scholar
Cowey, C. B., Knox, D., Adron, J. W., George, S. & Pirie, B. (1977). British Journal of Nutrition 38, 127–135.CrossRefGoogle Scholar
Denkla, W. D. & Dewey, H. K. (1967). Journal of Laboratory Clinical Medicine 69, 160–169.Google Scholar
Feigelson, P. & Greengard, O. (1962). Journal of Biological Chemistry 237, 3714–3717.CrossRefGoogle Scholar
Fuller, R. W. & Roush, B. W. (1973). Comparative Biochemistry and Physiology B, 46, 273–276.CrossRefGoogle Scholar
Garlick, P. J., Waterlow, J. C. & Swick, R. W. (1976). Biochemical Journal 156, 657–663.CrossRefGoogle Scholar
Halver, J. E. (1965). Federation Proceedings 24, 229.Google Scholar
Halver, J. E. (1982). Comparative Biochemistry and Physiology B, 73, 43–50.CrossRefGoogle Scholar
Kaushik, S. (1979). In Finfish Nutrition and Fishfeed Technology, pp. 197–208 [Halver, E.J., Tiews, K., editors]. Berlin: Heenemann.Google Scholar
Ketola, H. G. (1982). Comparative Biochemistry and Physiology B, 73, 17–24.CrossRefGoogle Scholar
Kloppel, T. M. & Post, G. (1975). Journal of Nutrition 105, 861–866.CrossRefGoogle Scholar
Knox, D., Cowey, C. B. & Adron, J. W. (1981). British Journal of Nutrition 45, 137–148.CrossRefGoogle Scholar
Knox, D., Cowey, C. B. & Adron, J. W. (1983). British Journal of Nutrition 50, 121–127.CrossRefGoogle Scholar
Lewis, A. J., Peo, E. R., Cunningham, P. J. & Moser, B. D. (1977). Journal of Nutrition 107, 1361–1368.CrossRefGoogle Scholar
McLaughlan, J. M. & Illman, W. E. (1967). Journal of Nutrition 93, 21–24.CrossRefGoogle Scholar
Nose, T. (1979). In Finfish Nutrition and Fishfeed Technology, pp. 145–156 [Halver, E.J., Tiews, K., editors]. Berlin: Heenemann.Google Scholar
Poston, H. A. & Combs, G. F. (1980). Proceedings of The Society of Experimental Biology and Medicine 163, 452–454.CrossRefGoogle Scholar
Robinson, E. H., Wilson, R. P. & Poe, W. E. (1981). Journal of Nutrition 111, 46–52.CrossRefGoogle Scholar
Sidransky, H., Verney, E. & Murty, C. N. (1981). Journal of Nutrition 111, 1942–1948.CrossRefGoogle Scholar
Steel, R. G. D. & Torrie, J. H. (1980). Principles and Procedures of Statistics. New York: McGraw–Hill.Google Scholar
Walton, M. J., Cowey, C. B. & Adron, J. W. (1982). Journal of Nutrition 112, 1525–1535.CrossRefGoogle Scholar
Wilson, R. P., Allen, O. W., Robinson, E. H. & Poe, W. E. (1978). Journal of Nutrition 108, 1595–1599.CrossRefGoogle Scholar
Wittman, J. S. (1976). Journal of Nutrition 106, 631–635.CrossRefGoogle Scholar
Yamada, S., Simpson, K. L., Tonaka, Y. & Katayama, T. (1981). Bulletin of the Japanese Society of Scientific Fisheries 47, 1035–1040.CrossRefGoogle Scholar
Yang, J. N. Y. & Carlson, J. R. (1972). Journal of Nutrition 102, 1655–1665.CrossRefGoogle Scholar
Young, V. R. & Munro, H. N. (1973). Journal of Nutrition 103, 1756–1763.CrossRefGoogle Scholar