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Effect of parsley (Petroselinum crispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects

Published online by Cambridge University Press:  09 March 2007

S. E. Nielsen ,
J. F. Young ,
B. Daneshvar ,
S. T. Lauridsen ,
P. Knuthsen ,
B. Sandström  and
L. O. Dragsted
S. E. Nielsen
Affiliation:
Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, Copenhagen, Denmark
J. F. Young
Affiliation:
Research Department of Human Nutrition Royal, Veterinary and Agricultural University, Frederiksberg, Denmark
B. Daneshvar
Affiliation:
Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, Copenhagen, Denmark
S. T. Lauridsen
Affiliation:
Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, Copenhagen, Denmark
P. Knuthsen
Affiliation:
Institute of Food Chemistry and Nutrition, Danish Veterinary and Food Administration, Copenhagen, Denmark
B. Sandström
Affiliation:
Research Department of Human Nutrition Royal, Veterinary and Agricultural University, Frederiksberg, Denmark
L. O. Dragsted*
Affiliation:
Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, Copenhagen, Denmark
*
*Corresponding author: Dr L. O. Dragsted, fax +45 33 95 60 01, email [email protected]
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Abstract

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Seven men and seven women participated in a randomized crossover trial to study the effect of intake of parsley (Petroselinum crispum), containing high levels of the flavone apigenin, on the urinary excretion of flavones and on biomarkers for oxidative stress. The subjects received a strictly controlled diet low in flavones and other naturally occurring antioxidants during the 2 weeks of intervention. This basic diet was supplemented with parsley providing 3·73–4·49 mg apigenin/MJ in one of the intervention weeks. Urinary excretion of apigenin was 1·59–409·09 μg/MJ per 24 h during intervention with parsley and 0–112·27 μg/MJ per 24 h on the basic diet (P < 0·05). The fraction of apigenin intake excreted in the urine was 0·58 (se 0·16) % during parsley intervention. Erythrocyte glutathione reductase (EC 1.6.4.1; GR) and superoxide dismutase (EC 1.15.1.1; SOD) activities increased during intervention with parsley (P < 0·005) as compared with the levels on the basic diet, whereas erythrocyte catalase (EC 1.11.1.6) and glutathione peroxidase (EC 1.11.1.9) activities did not change. No significant changes were observed in plasma protein 2-adipic semialdehyde residues, a biomarker of plasma protein oxidation. In this short-term investigation, an overall decreasing trend in the activity of antioxidant enzymes was observed during the 2-week study. The decreased activity of SOD was strongly correlated at the individual level with an increased oxidative damage to plasma proteins. However, the intervention with parsley seemed, partly, to overcome this decrease and resulted in increased levels of GR and SOD.

Keywords

ApigeninAntioxidant statusUrinary excretion
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 6 , June 1999 , pp. 447 - 455
Copyright
Copyright © The Nutrition Society 1999

References

Ameer, B, Weintraub, RA, Johnson, JV, Yost, RA & Rouseff, RL (1996) Flavanone absorption after naringin, hesperidin, and citrus administration. Clinical Pharmacology and Therapeutics 60, 34–40.CrossRefGoogle ScholarPubMed
Armitage, P & Berry, G (1994) Statistical Methods in Medical Research, 3rd ed. Cambridge: Blackwell Science Ltd.Google Scholar
Belsten, JL & Wright, AJ (1995) European community-FLAIR common assay for erythrocyte superoxide dismutase (SOD); results of an inter-laboratory trial. European Journal of Clinical Nutrition 49, 928–931.Google ScholarPubMed
Birt, DF, Mitchell, D, Gold, B, Pour, P & Pinch, HC (1997) Inhibition of ultraviolet light-induced skin carcinogenesis in SKH-1 mice by apigenin, a plant flavonoid. Anticancer Research 17, 85–91.Google ScholarPubMed
Bro, S, Sandström, B & Heydorn, K (1990) Intake of essential and toxic trace elements in a random sample of Danish men as determined by the duplicate portion sample technique. Journal of Trace Elements and Electrolytes in Health and Disease 4, 147–155.Google Scholar
Daneshvar, B (1996) Biomarkers for oxidative damage to proteins. Development of analytical methods and their application to biological samples. PhD Thesis, Institute of Pharmacology, University of Copenhagen, Denmark.Google Scholar
Daneshvar, B, Frandsen, H, Autrup, H & Dragsted, LO (1997) Gamma-glutamyl semialdehyde and 2-amino-adipic semialdehyde: biomarkers of oxidative damage to proteins. Biomarkers 2, 117–123.CrossRefGoogle Scholar
Dixon, ZR, Burri, BJ, Clifford, A, Frankel, EN, Schneeman, BO, Parks, E, Keim, NL, Barbieri, T, Wu, MM, Fong, AK, Kretsch, MJ, Sowell, AL & Erdman, JW Jr (1994) Effects of α carotene-deficient diet on measures of oxidative susceptibility and superoxide dismutase activity in adult women. Free Radical Biology and Medicine 17, 537–544.CrossRefGoogle Scholar
Fernandez de Simon, B, Perez Ilzarbe, J, Hernandez, T, Gomez Cordoves, C & Estrella, I (1992) Importance of phenolic compounds for the characterization of fruit juices. Journal of Agricultural and Food Chemistry 40, 1531–1535.CrossRefGoogle Scholar
Fraga, CG, Martino, VS, Ferraro, GE, Coussio, JD & Boveris, A (1987) Flavonoids as antioxidants evaluated by in vitro and in situ liver chemiluminescence. Biochemical Pharmacology 36, 717–720.CrossRefGoogle ScholarPubMed
Hart, DJ & Scott, KJ (1995) Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Food Chemistry 54, 101–111.CrossRefGoogle Scholar
Hollman, PCH, van der Gaag, MS, Mengelers, MJB, van Trijp, JMP, de Vries, JHM & Katan, MB (1996) Absorption and disposition kinetics of the dietary antioxidant quercetin in man. Free Radical Biology and Medicine 21, 703–707.CrossRefGoogle ScholarPubMed
Hollman, PCH, van Trijp, JMP, Buysman, MNCP, van der Gaag, MS, Mengelers, MJB, Vries, JHM & Katan, MB (1997) Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. FEBS Letters 418, 152–156.CrossRefGoogle ScholarPubMed
Hollman, PCH, Vries, JHM, van Leeuwen, SD, Mengelers, MJB & Katan, MB (1995) Absorption of dietary quercetin glycosides and quercetin in healthy ileostomy volunteers. American Journal of Clinical Nutrition 62, 1276–1282.CrossRefGoogle ScholarPubMed
Janssen, K, Mensink, RP, Cox, FJJ, Harryvan, JL, Hovenier, R, Hollman, PCH & Katan, MB (1998) Effects of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in vitro and a dietary supplement study. American Journal of Clinical Nutrition 67, 255–262.CrossRefGoogle Scholar
Justesen, U, Knuthsen, P & Leth, T (1998) Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by HPLC with photodiode array and mass spectrometric detection. Journal of Chromatography A 799, 101–110.CrossRefGoogle ScholarPubMed
Kühnau, J (1976) The flavonoids. A class of semi-essential food components: their role in human nutrition. World Review of Nutrition and Dietetics 24, 117–191.CrossRefGoogle ScholarPubMed
Kuo, ML, Lee, KC & Lin, JK (1992) Genotoxicities of nitropyrenes and their modulation by apigenin, tannic acid, ellagic acid and indole-3-carbinol in the Salmonella and CHO systems. Mutation Research 270, 87–95.CrossRefGoogle ScholarPubMed
Lee, SJ, Son, KH, Chang, HW, Do, JC, Jung, KY, Kang, SS & Kim, HP (1993) Antiinflammatory activity of naturally occurring flavone and flavonol glycosides. Archives of Pharmacology Research 16, 25–28.CrossRefGoogle Scholar
Lux, O & Naidoo, D (1995) Biological variability of superoxide dismutase and glutathione peroxidase in blood. Redox Report 1, 331–335.CrossRefGoogle ScholarPubMed
Milne, DB & Nielsen, FH (1996) Effects of a diet low in copper on copper-status indicators in postmenopausal women. American Journal of Clinical Nutrition 63, 358–364.CrossRefGoogle ScholarPubMed
Møller, A (1996) Levnedsmiddeltabeller (The Composition of Foods), 4th revised and extended ed. [Saxholt, E, editor]. Copenhagen: National Food Agency of Denmark.Google Scholar
Nielsen, SE & Dragsted, LO (1998) Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection. Journal of Chromatography B 713, 379–386.CrossRefGoogle ScholarPubMed
Pocock, SJ (1998) Clinical trials. A practical approach. Chichester: John Wiley & Sons.Google Scholar
Saghir, AN, Rickards, H & Pall, HS (1997) Postprandial changes in superoxide dismutase activity in subjects with Gilles de la Tourette syndrome and controls. Experimental Neurology 144, 420–422.CrossRefGoogle ScholarPubMed
Tschiersch, K & Hölzl, J (1993) Resorption und Ausscheidung von Apigenin, Apigenin-7-glucosid und Herniarin nach Peroraler Gabe eines Extract von Matricaria recutita (L.) [syn. Chamomilla recutita (L.) Rauschert] (Absorption and excretion of apigenin, apigenin-7-glycoside and herniarin after oral administration of extracts of Matricaria recutita). Pharmazie 48, 554–555.Google ScholarPubMed
van Acker, SABE, van den Berg, DJ, Tromp, MNJL, Griffioen, DH, van Bennekom, WP, van der Vijgh, WJF & Bast, A (1996) Structural aspects of antioxidant activity of flavonoids. Free Radicical Biology and Medicine 20, 331–342.CrossRefGoogle ScholarPubMed
Wei, H, Tye, L, Bresnick, E & Birt, DF (1989) Inhibitory effect of apigenin, a plant flavonoid, on epidermal ornithine decarboxylase and skin tumor promotion in mice. Cancer Research 50, 499–502.Google Scholar
Wheeler, CR, Salzman, JA, Elsayed, NM, Omaye, ST & Korte, DW Jr (1990) Automated assay for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase. Analytical Biochemistry 184, 193–199.CrossRefGoogle ScholarPubMed
Young, JF, Nielsen, SE, Haraldsdóttir, J, Daneshvar, B, Lauridsen, ST, Knuthsen, P, Crozier, A, Sandström, B & Dragsted, LO (1999) Effect of juice intake on urinary quercetin excretion and biomarkers of antioxidative status. American Journal of Clinical Nutrition 69, 87–94.CrossRefGoogle ScholarPubMed