Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-23T21:23:05.365Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of frequency of feeding of diets containing free or protein-bound lysine on the oxidation of [14C]lysine or [14C]phenylalanine by growing pigs

Published online by Cambridge University Press:  09 March 2007

E. S. Batterham  and
H. S. Bayley
E. S. Batterham
Affiliation:
Department of Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
H. S. Bayley
Affiliation:
Department of Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of frequency of feeding (once daily or six equal portions at intervals of 3 h) of diets containing lysine (6.4 g/kg) supplied as either protein-bound or free lysine on the metabolism of essential amino acids was investigated in pigs over the 23–34 kg weight range. The amounts of radioactivity from 14CO2 produced by the pigs given oral doses of [14C]lysine or [14C]phenylalanine were determined. [14C]Lysine was confirmed to be unsuitable presumably because the oral dose was diluted by the dietary free lysine or was absorbed ahead of the protein-bound lysine, or both. With [14C]phenylalanine addition, there was a significantly (P < 0.01) greater production of 14CO2 by pigs fed once daily on the diet containing free lysine. However, with frequent feeding, there was no significant difference (P > 0.05) in the amount of 14CO2 produced by pigs from [14C]phenylalanine for either diet. Growth responses of the pigs were lower when the diet containing free lysine was given once daily (P < 0.01) but were similar when the diets were given frequently. It is concluded that the more rapid absorption of free lysine by growing pigs fed once daily results in an imbalance of amino acids at the sites of metabolism. This leads to greater oxidation of essential amino acids than occurs when all the amino acids are supplied in the protein-bound form. With frequent feeding, a better balanced supply of amino acids is absorbed, leading to similar rates of oxidation of excess essential amino acids from diets containing either free or protein-bound lysine.

Keywords

Feeding frequencyLysineAmino acid oxidationPig
Type
Protein and Peptide Metabolism
Information
British Journal of Nutrition , Volume 62 , Issue 3 , November 1989 , pp. 647 - 655
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

Agricultural Research Council (1981). The Nutrient Requirement of Pigs, 2nd ed. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Association of Official Analytical Chemists (1975). Official Methods of Analysis of the Association of Official Analytical Chemists, 12th ed. Washington, DC: Association of Official Analytical Chemists.Google Scholar
Batterham, E.S. & Murison, R.D. (1981). Utilization of free lysine by growing pigs. British Journal of Nutrition 46, 87–92.CrossRefGoogle ScholarPubMed
Fuller, M.F. & Wang, T.C. (1987). Amino acid requirements of the growing pig. In Manipulating Pig Production, pp. 97–111 [Barnett, J.L., Batterham, E.S., Cronin, G.M., Hansen, C., Hemsworth, P.H., Hennessy, D.P., Hughes, P.E., Johnston, N.E. and King, R.H., editors]. Ferntree Gully: JFL Promotions.Google Scholar
Hugli, T.E. & Moore, S. (1972). Determination of the tryptophan content of proteins by ion-exchange chromatography of alkaline hydrolysates. Journal of Biological Chemistry 247, 2828–2834.CrossRefGoogle ScholarPubMed
Kim, K., McMillan, I. & Bayley, H.S. (1983). Determination of amino acid requirements of young pigs using an indicator amino acid. British Journal of Nutrition 50, 369–382.CrossRefGoogle ScholarPubMed
Williams, K.C. & Dunkin, A.C. (1980). Utilization of synthetic lysine by the growing pig. Proceedings of the Australian Society of Animal Production 13, 149–152.Google Scholar