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Effect of dietary lactose on salt-mediated changes in mineral metabolism and bone composition in the rat

Published online by Cambridge University Press:  09 March 2007

C. Shortt  and
A. Flynn
C. Shortt
Affiliation:
Department of Nutrition, University College, Cork, Republic of Ireland
A. Flynn
Affiliation:
Department of Nutrition, University College, Cork, Republic of Ireland
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Abstract

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The effects of salt (sodium chloride) supplementation of rat diets (80 g/kg diet), with or without lactose (150 g/kg), were studied in weanling rats over 14 d. Dietary salt increased water intake and reduced weight gain and food conversion efficiency, but these variables were unaffected by lactose. Salt-supplemented rats exhibited a three- to fivefold increase in urinary calcium excretion and a small increase in urinary magnesium and phosphorus excretion, irrespective of dietary lactose content. In addition, salt supplementation reduced plasma alkaline phosphatase (EC 3.1.3.1) activity. Lactose increased urinary Ca and Mg excretion and plasma Ca and P concentrations. Salt reduced tibia mass but not tibia mass expressed relative to body-weight, but neither variable was affected by lactose. Both tibia Mg content and concentration were reduced by salt but unaffected by lactose, and neither tibia P content nor concentration was affected by salt or lactose. Tibia Ca content was reduced by salt but this was prevented by lactose. Tibia Ca concentration was unaffected by salt or lactose, although there was a reduction (not significant) in tibia Ca concentration in animals fed on the lactose-free diet. These results show that lactose had no independent effect on bone and that reduced accretion of bone mass and mineral content in rats fed on the high-salt diets was due, at least in part, to reduced growth. Failure to offset sodium-induced hypercalciuria by a compensatory increase in net Ca absorption may have contributed to reduced bone Ca accretion. The protective effect of lactose against reduced bone Ca accretion may be due to increased Ca absorption.

Keywords

SaltLactoseCalciumBoneRat
Type
Bioavailability and Utilization of Inorganic Nutrients
Information
British Journal of Nutrition , Volume 66 , Issue 1 , July 1991 , pp. 73 - 81
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

American Institute of Nutrition (1977). Ad Hoc Committee on Standards for Nutritional Studies. Report of the Committee. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Antoniou, L., Eisner, G., Slotkoff, L. & Lilienfield, L. (1969). Relationship between sodium and calcium transport in the kidney. Journal of Laboratory and Clinical Medicine 74, 410–420.Google ScholarPubMed
Au, W. Y. & Raisz, L. G. (1967). Restoration of parathyroid responsiveness in vitamin D-deficient rats by parenteral calcium or dietary lactose. Journal of Clinical Investigation 46, 1572–1578.CrossRefGoogle ScholarPubMed
Baker, H. J., Lindsey, R. J. & Weisbroth, H. (1979). Housing to control research variables. In The Laboratory Rat, vol. 1, pp. 169–187 [Baker, H. J., Lindsey, R. J. and Weisbroth, H., editors]. London: Academic Press.CrossRefGoogle Scholar
Bessey, O. A., Lowry, O. H. & Brock, M. J. (1946). A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. Journal of Biological Chemistry 164, 321–332.CrossRefGoogle ScholarPubMed
Breslau, N. A., McGuire, J. L., Zerwekh, J. E. & Pak, C. Y. C. (1982). The role of dietary sodium in renal excretion and intestinal absorption of calcium and in vitamin D metabolism. Journal of Clinical Endocrinology and Metabolism 55, 369–373.CrossRefGoogle ScholarPubMed
Breslau, N. A., Sakhaee, K. & Pak, C. Y. C. (1985). Impaired adaptation to salt-induced urinary calcium losses in postmenopausal osteoporosis. Transactions of the Association of American Physicians 98, 107–115.Google ScholarPubMed
Coe, F. L., Firpo, J.J., Hollandsworth, D. L., Segil, L., Canterbury, J. M. & Reiss, E. (1975). Effects of acute and chronic metabolic acidosis on serum immunoreactive parathyroid hormone in man. Kidney International 8, 262–273.CrossRefGoogle ScholarPubMed
Condon, J. R., Nassim, J. R., Millard, F. J. C., Hilbe, A. & Stainthorpe, E. M. (1970). Calcium and phosphorus metabolism in relation to lactose tolerance. Lancet i, 1027–1029.CrossRefGoogle Scholar
Debiec, H. & Lorenc, R. (1988). Influence of lactose on phosphate metabolism in rats. British Journal of Nutrition 59, 87–92.CrossRefGoogle ScholarPubMed
Forbes, R. M. (1964). Mineral utilization in the rat. III. Effects of calcium, phosphorus, lactose and source of protein in zinc-deficient and in zinc-adequate diets. Journal of Nutrition 83, 225–233.CrossRefGoogle Scholar
Goulding, A. (1980 a). Effects of sodium chloride supplements on tibial calcium content in rats taking a low-calcium diet with a moderate or a high protein intake. Proceedings of the University of Otago Medical School 58, 13–14.Google Scholar
Goulding, A. (1980 b). Effects of dietary NaCl supplements on parathyroid function, bone turnover and bone composition in rats taking restricted amounts of calcium. Mineral Electrolyte Metabolism 4, 203–208.Google Scholar
Goulding, A. & Campbell, D. R. (1982). Generalized skeletal loss of calcium induced by oral sodium chloride supplements in adult oophorectomized rats consuming a low-calcium diet. Proceedings of the University of Otago Medical School 60, 3–4.Google Scholar
Goulding, A. & Campbell, D. R. (1983). Dietary NaCl loads promote calciuria and bone loss in adult oophorectomized rats consuming a low calcium diet. Journal of Nutrition 113, 1409–1414.CrossRefGoogle ScholarPubMed
Goulding, A. & Campbell, D. R. (1984). Effects of oral loads of sodium chloride on bone composition in growing rats consuming ample dietary calcium. Mineral Electrolyte Metabolism 10, 58–62.Google Scholar
Goulding, A., Everitt, H., Cooney, J. & Spears, G. (1986). Sodium and Osteoporosis. In Recent Advances in Clinical Nutrition, vol. 2, pp. 99–108 [Wahlqvist, M. and Truswell, A., editors]. London: John Libbey.Google Scholar
Goulding, A. & Gold, E. (1986). Effects of dietary sodium chloride loading on parathyroid function, 1,25-dihydroxy vitamin D, calcium balance, and bone metabolism in female rats during chronic prednisolone administration. Endocrinology 119, 2148–2154.CrossRefGoogle Scholar
Goulding, A. & Gold, E. (1988). Effects of dietary NaCl supplementation on bone synthesis of hydroxyproline, urinary hydroxyproline excretion and bone 45Ca uptake in the rat. Hormone and Metabolic Research 20, 734–745.CrossRefGoogle Scholar
Goulding, A. & McIntosh, J. (1986). Effects of NaCl on calcium balance, parathyroid function, and hydroxyproline excretion in prednisolone-treated rats consuming low calcium diet. Journal of Nutrition 116, 1037–1044.CrossRefGoogle ScholarPubMed
Greger, J. L., Krashoc, C. L. & Krzykowski, C. E. (1987). Calcium, sodium and chloride interaction in rats. Nutrition Research 7, 401–412.CrossRefGoogle Scholar
Klein, L., Lafferty, F. W., Pearson, O. H. & Curtiss, P. H. (1964). Correlation of urinary hydroxyproline, serum alkaline phosphatase and skeletal calcium turnover. Metabolism 13, 272–284.CrossRefGoogle ScholarPubMed
Law, L. K., Swaminathan, R. & Donnan, S. P. B. (1988). Relationship between sodium excretion and calcium excretion in healthy subjects. Medical Science Research 16, 643.Google Scholar
McCarron, D. A., Rankin, L. I., Bennett, W. M., Krutzik, S., McClung, M. R. & Luft, F. C. (1981). Urinary calcium excretion at extremes of sodium intake in normal man. American Journal of Nephrology 1, 84–90.CrossRefGoogle ScholarPubMed
McParland, B. E., Goulding, A. & Campbell, A. J. (1989). Dietary salt affects biochemical markers of resorption and formation of bone in elderly women. British Medical Journal 299, 834–835.CrossRefGoogle ScholarPubMed
Marie, P. J. & Travers, R. (1983). Effects of magnesium and lactose supplementation on bone metabolism in the X-linked hypophosphatemic mouse. Metabolism 32, 165–171.CrossRefGoogle Scholar
Meyer, W. J., Transbol, I., Bartter, F. C. & Delea, C. (1976). Control of calcium absorption. Effect of sodium chloride loading and depletion. Metabolism 25, 989–993.CrossRefGoogle ScholarPubMed
Miller, S. C., Miller, M. A. & Omura, T. H. (1988). Dietary lactose improves endochondral growth and bone development and mineralization in rats fed a vitamin D-deficient diet. Journal of Nutrition 118, 72–77.CrossRefGoogle ScholarPubMed
Mitruka, B. M. & Rawnsley, H. M. (1977). Clinical biochemistry. In Clinical, Biochemical and Haematological Reference Values in Normal Experimental Animals, Section 5, pp. 117–129 [Mitruka, B. M. and Rawnsley, H. M., editors]. New York: Masson Publishing.Google Scholar
Nordin, B. E. C. & Polley, K. J. (1987). Metabolic consequences of the menopause. Calcified Tissues International 41, S1–S59.Google ScholarPubMed
Pernot, F., Berthelot, A., Schleiffer, R. & Gairard, A. (1979). Ionized serum calcium, urinary cAMP and immunoreactive PTH after DOCA + NaCl treatment in the rat. Mineral Electrolyte Metabolism 2, 258.Google Scholar
Schaafsma, G., Dekker, P. R. & de Waard, H. (1988). Nutritional aspects of yogurt. 2. Biovavailability of essential minerals and trace elements. Netherlands Milk and Dairy Journal 42, 135–146.Google Scholar
Schaafsma, G. & Visser, R. (1980). Nutritional interrelationships between calcium, phosphorus and lactose in rats. Journal of Nutrition 110, 1101–1111.CrossRefGoogle ScholarPubMed
Shortt, C. & Flynn, A. (1990). The sodium-calcium interrelationship with particular reference to osteoporosis. Nutrition Research Reviews 3, 101–115.CrossRefGoogle Scholar
Shortt, C., Madden, A., Flynn, A. & Morrissey, P. A. (1988). The influence of dietary sodium chloride intake on urinary calcium on selected Irish individuals. European Journal of Clinical Nutrition 42, 595–603.Google Scholar
Snedecor, G. W. & Cochran, W. G. (1967). Statistical Methods. Ames, Iowa: Iowa State University Press.Google Scholar
Taylor, A. K., Lundy, M. W., Libanati, C. R. & Baylink, D. J. (1988). Diagnostic tests for metabolic bone and mineral disorders. In Metabolic Bone and Mineral Disorders, pp. 33–62 [Manolagas, S. C. and Olefsky, J. M., editors]. New York: Churchill Livingstone.Google Scholar
Weissman, N. & Pileggi, V. J. (1974). Inorganic ions. In Clinical Chemistry: Principles and Technics, pp. 639–754 [Henry, R. J., Cannon, D. C. and Winkelman, J. W., editors]. Maryland: Harper & Row.Google Scholar
Whiting, S. J. & Cole, D. E. (1986). Effect of dietary anion composition on acid-induced hypercalciuria in the adult rat. Journal of Nutrition 116, 388–394.CrossRefGoogle ScholarPubMed