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Effect of composition of ruminally-infused short-chain fatty acids on net fluxes of nutrients across portal-drained viscera in underfed ewes

Published online by Cambridge University Press:  09 March 2007

Pierre Nozière ,
Cécile Martin ,
Didier Rémond ,
Niels B. Kristensen ,
Richard Bernard  and
Michel Doreau
Pierre Nozière*
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Cécile Martin
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Didier Rémond
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Niels B. Kristensen
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Box 50, DK-8830 Tjele, Denmark
Richard Bernard
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Michel Doreau
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
*
*Corresponding author: Dr Pierre Nozière, fax +33 4 73 62 45 19, email [email protected]
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Abstract

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Four ewes, each fitted with a rumen cannula and with catheters in the mesenteric artery and portal and mesenteric veins, received continuous intrarumen infusions of water or of short-chain fatty acids (SCFA). SCFA infusions were isoenergetic (83 kJ/h) and provided rumen molar proportions (acetate : propionate : butyrate) of 70 : 20 : 10, 50 : 40 : 10 or 50 : 20 : 30. The rumen SCFA production rate with the basal diet was 90·0, 23·1 and 8·8 mmol/h for acetate, propionate and butyrate respectively. Portal net fluxes indicated that 74, 67 and 22–30 % of infused acetate, propionate and butyrate respectively, reached the portal vein. Portal net release of β-hydroxybutyrate increased with SCFA infusions, irrespective of the amount of butyrate infused. Portal net release of lactate decreased with high-butyrate infusion. Portal net uptake of glucose increased with the SCFA infusions. In ewes infused with water, a portal net uptake of total amino acids (AA) was observed. SCFA infusions decreased the uptake of nonessential AA (glutamate, glycine, but not glutamine) and increased the net release of tyrosine and essential AA (isoleucine, leucine). Portal net fluxes of AA were similar with both high-acetate and high-propionate infusions. Lower net uptake of glutamine and net release of most essential AA and some nonessential AA were observed with the high-butyrate infusion. Energetic summation of portal net release was not significantly different between the three SCFA infusions, although it tended to be lower with high-butyrate infusion. This may be related to the higher trophic effect of butyrate on the digestive mucosa.

Keywords

SheepShort-chain fatty acidsPortal fluxesAmino acids
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 5 , May 2000 , pp. 521 - 531
Copyright
Copyright © The Nutrition Society 2000

References

Barnes, RJ, Comline, RS & Dobson, A (1986) The control of splanchnic blood flow. In Control of Digestion and Metabolism in Ruminants, pp. 41–59 [Milligan, LP, Grovum, WL and Dobson, A, editors]. Englewood Cliffs, NJ: Prentice Hall.Google Scholar
Barnouin, J, El Idilbi, N, Chilliard, Y, Chacornac, JP and Lefaivre, R (1986) Micro-dosage automatisé sans déprotéinisation du 3-hydroxybutyrate plasmatique chez les bovins (Automated micro-analysis of bovine plasma 3-hydroxybutyrate). Annales de Recherches Vétérinaires 17, 129–139.Google Scholar
Bergman, EN (1990) Energy contributions of volatile fatty acids from the gastrointestinal tract in various species. Physiological Reviews 70, 567–590.CrossRefGoogle ScholarPubMed
Binnerts, WT Van't Klooster ATh and Frens, AM (1968) Soluble chromium indicator measured by atomic absorption in digestion experiments. Veterinary Record 82, 470.Google Scholar
Bratton, CA and Marshall, EK (1939) A new coupling component for sulfanil-amide determination. Journal of Biological Chemistry 128, 537–550.CrossRefGoogle Scholar
Brighenti, F (1997) Simple method for quantitative analysis of short chain fatty acids in serum by gas-liquid chromatography. In Plant Polysaccharides in Human Nutrition: Structure, Function, Digestive Fate and Metabolic Effects, pp. 114–119 [Guillon, F, Abraham, G, Amado, R, Andersson, H, Asp, HG, Bach Knudsen, KE, Champ, M and Robertson, J, editors]. Nantes: INRA.Google Scholar
Bueno, L (1972) Le rumen isolé in situ: absorption des acides gras volatils (The isolated in situ rumen: absorption of volatile fatty acids). Revue de Médecine Vétérinaire 123, 943–953.Google Scholar
Casse, EA, Rulquin, H and Huntington, GB (1994) Effect of mesenteric vein infusion of propionate on splanchnic metabolism in primiparous Holstein cows. Journal of Dairy Science 77, 3296–3303.CrossRefGoogle ScholarPubMed
Chilliard, Y, Bocquier, F and Doreau, M (1998) Digestive and metabolic adaptations of ruminants to undernutrition, and consequences on reproduction. Reproduction Nutrition Development 38, 131–152.CrossRefGoogle ScholarPubMed
Demigné, C, Yacoub, C, Morand, C and Remesy, C (1991) Interactions between propionate and amino acid metabolism in isolated sheep hepatocytes. British Journal of Nutrition 65, 301–317.CrossRefGoogle ScholarPubMed
Dijkstra, J, Boer, H Van Bruchem, J, Bruining, M and Tamminga, S (1993) Absorption of volatile fatty acids from the rumen of lactating dairy cows as influenced by volatile fatty acid concentration, pH and rumen liquid volume. British Journal of Nutrition 69, 385–396.CrossRefGoogle ScholarPubMed
Eisemann, JH and Huntington, GB (1994) Metabolite flux across portal-drained viscera, liver, and hindquarters of hyperinsulinemic, euglycemic beef steers. Journal of Animal Science 72, 2919–2929.CrossRefGoogle ScholarPubMed
Goetsch, AL (1998) Splanchnic tissue energy use in ruminants that consume forage-based diets ad libitum. Journal of Animal Science 76, 2737–2746.CrossRefGoogle ScholarPubMed
Gross, KL, Harmon, DL and Avery, TB (1990) Portal drained viscera flux of nutrients in lambs fed alfalfa or maintained by total intragastric infusion. Journal of Animal Science 68, 214–221.CrossRefGoogle ScholarPubMed
Gross, KL, Harmon, DL, Minton, JE and Avery, TB (1990) Effects of isoenergetic infusions of propionate and glucose on portal drained viscera nutrient flux and concentrations of hormones in lambs maintained by total intragastric infusion. Journal of Animal Science 68, 2566–2574.CrossRefGoogle ScholarPubMed
Harmon, DL and Avery, TB (1987) Effects of dietary monensin and sodium propionate on net nutrient flux in steers fed a high-concentrate diet. Journal of Animal Science 65, 1610–1616.Google ScholarPubMed
Hogan, JP and Weston, RH (1967) The digestion of chopped and ground roughages by sheep. II. The digestion of nitrogen and some carbohydrate fractions in the stomach and intestines. Australian Journal of Agricultural Research 18, 803–819.CrossRefGoogle Scholar
Huhtanen, P and Jaakkola, S (1995) Intraruminal infusion technique for the estimation of ruminal VFA production. Annales de Zootechnie 44(Suppl.), 168.CrossRefGoogle Scholar
Huhtanen, P, Miettinen, H and Ylinen, M (1993) Effect of increasing ruminal butyrate on milk yield and blood constituents in dairy cows fed a grass silage-based diet. Journal of Dairy Science 76, 1114–1124.CrossRefGoogle ScholarPubMed
Huntington, GB (1990) Energy metabolism in the digestive tract and liver of cattle: influence of physiological state and nutrition. Reproduction Nutrition Development 30, 35–47.CrossRefGoogle ScholarPubMed
Huntington, GB and Prior, RL (1985) Net absorption of amino acids by portal-drained viscera and hind half of beef cattle fed a high concentrate diet. Journal of Animal Science 60, 1491–1499.CrossRefGoogle ScholarPubMed
Huntington, GB, Reynolds, PJ and Tyrrell, HF (1983) Net absorption and ruminal concentrations of metabolites in nonpregnant dry Holstein cows before and after intraruminal acetic acid infusion. Journal of Dairy Science 66, 1901–1908.CrossRefGoogle ScholarPubMed
Institut National de la Recherche Agronomique (1989) Ruminant Nutrition Recommended Allowances and Feed Tables [Jarrige, R, editor]. Paris: INRA/John Libbey Eurotext.Google Scholar
Isserty, A, Ortigues, I and Rémond, D (1998) Mesure des débits splanchniques par dilution de marqueur: comparaison de quatre méthodes de dosage de l'acide para-amino-hippurique (Measurement of splanchnic blood flows by marker dilution: comparison of four analytical methods of para-amino-hippuric acid). Reproduction Nutrition Development 38, 93–106.CrossRefGoogle Scholar
Jouany, JP (1982) Volatile fatty acids and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermentor contents. Sciences des Aliments 2, 131–144.Google Scholar
Katz, ML and Bergman, EN (1969) Simultaneous measurements of hepatic and portal blood flow in the sheep and dog. American Journal of Physiology 216, 946–952.CrossRefGoogle ScholarPubMed
Krehbiel, CR, Harmon, DL and Schnieder, JE (1992) Effect of increasing ruminal butyrate on portal and hepatic nutrient flux in steers. Journal of Animal Science 70, 904–914.Google ScholarPubMed
Kristensen, NB, Danfaer, A, Tetens, V and Agergaard, N (1996) Portal recovery of intraruminally infused short-chain fatty acids in sheep. Acta Agriculturae Scandinavica Section A Animal Science 46, 26–38.Google Scholar
Kristensen, NB, Pierzynowsky, S and Danfaer, A (1999) Net portal appearance of volatile fatty acids in sheep intraruminally infused with mixtures of acetate, proprionate, isobutyrate, butyrate and valerate Journal of Animal Science.Google Scholar
Leng, RA, Steel, JW and Luick, JR (1967) Contribution of propionate to glucose synthesis in sheep. Biochemical Journal 103, 785–790.CrossRefGoogle ScholarPubMed
Lindsay, DR (1993) Metabolism of the portal drained viscera. In Quantitative Aspects of Ruminant Digestion and Metabolism, pp. 267–289 [Forbes, JM, and France, J, editors]. Wallingford: CAB International.Google Scholar
Lomax, MA and Baird, GD (1983) Blood flow and nutrient exchange across the liver and gut of the dairy cow. Effects of lactation and fasting. British Journal of Nutrition 49, 481–496.CrossRefGoogle ScholarPubMed
Marsh, WH, Fingerhut, B and Miller, H (1965) Automated and manual direct methods for the determination of blood urea. Clinical Chemistry 11, 624–627.CrossRefGoogle ScholarPubMed
Miettinen, H and Huhtanen, P (1996) Effects of the ratio of ruminal propionate to butyrate on milk yield and blood metabolites in dairy cows. Journal of Dairy Science 79, 851–861.Google ScholarPubMed
Mould, FL and Ørskov ER (1983) Manipulation of rumen fluid pH and its influence on cellulolysis in sacco, dry matter degradation rumen microflora of sheep offered either hay or concentrate. Animal Feed Science and Technology 10, 1–14.Google Scholar
Nozière, P, Attaix, D, Bocquier, F and Doreau, M (1999) Effects of underfeeding and refeeding on weight and metabolism of splanchnic organs in ewes. Journal of Animal Science 77, 2279–2290.CrossRefGoogle ScholarPubMed
Nozière, P, Rémond, D, Bernard, L and Doreau, M (1999) Effect of underfeeding on portal net fluxes of glucose, lactate, ammonia, urea and amino acids in ewes. South African Journal of Animal Science 29, 236–237.Google Scholar
Nozière, P, Rémond, D, Ferlay, A and Doreau, M (1998) Differences between blood and plasma concentrations of acetate, β-hydroxybutyrate, glucose, ammonia and urea: implications for measurement of portal net fluxes in ewes. Reproduction Nutrition Development 38, 509–518.Google ScholarPubMed
Ortigues, I and Doreau, M (1995) Responses of the splanchnic tissues of ruminants to changes in intake: absorption of digestion end products, tissue mass, metabolic activity and implications to whole animal energy metabolism. Annales de Zootechnie 44, 321–346.CrossRefGoogle Scholar
Ortigues, I, Durand, D and Lefaivre, J (1994) Use of para-amino hippuric acid to measure blood flows through portal-drained-viscera, liver and hindquarters in sheep. Journal of Agricultural Science, Cambridge 122, 299–308.CrossRefGoogle Scholar
Peters, JP, Shen, RYW, Robinson, JA and Chester, ST (1990) Disappearance and passage of propionic acid from the rumen of the beef steer. Journal of Animal Science 68, 3337–3349.CrossRefGoogle ScholarPubMed
Rémond, D, Chaise, JP, Delval, E and Poncet, C (1993) Net flux of metabolites across the ruminal wall of sheep fed twice a day with orchardgrass hay. Journal of Animal Science 71, 2529–2538.CrossRefGoogle Scholar
Rémond, DOrtigues, I and Jouany, JP (1995) Energy substrates for the rumen epithelium. Proceedings of the Nutrition Society 54, 95–105.CrossRefGoogle ScholarPubMed
Rémond, D, Ortigues-Marty, I, Isserty, A and Lefaivre, J (1998) Technical note: Measuring portal blood flow in sheep using an ultrasonic transit time flow probe. Journal of Animal Science 76, 2712–2716.CrossRefGoogle ScholarPubMed
Reynolds, CK (1995) Quantitative aspects of liver metabolism in ruminants. In Ruminant Physiology: Digestion, Metabolism, Growth and Reproduction, pp. 351–371 [Engelhardt, WV, Leonhard-Marek, S, Breves, G and Giesecke, D, editors]. Stuttgart: Ferdinand Enke Verlag.Google Scholar
Reynolds, CK and Huntington, GB (1988) Partition of portal-drained visceral net flux in beef steers. 2. Net flux of volatile fatty acids, D-β-hydroxybutyrate and L-lactate across stomach and post-stomach tissues. British Journal of Nutrition 60, 553–562.CrossRefGoogle ScholarPubMed
Sakata, T and Tamate, H (1978) Rumen epithelial cell proliferation accelerated by rapid increase in intraruminal butyrate. Journal of Dairy Science 61, 1109–1113.CrossRefGoogle ScholarPubMed
Seal, CJ and Parker, DS (1994) Effect of intraruminal propionic acid infusion on metabolism of mesenteric- and portal-drained viscera in growing steers fed a forage diet: I. Volatile fatty acids, glucose, and lactate. Journal of Animal Science 72, 1325–1334.CrossRefGoogle ScholarPubMed
Shriver, BJ, Hoover, WH, Sargent, JP, Crawford, RJ and Thayne, WV (1986) Fermentation of a high concentrate diet as affected by ruminal pH and digesta flow. Journal of Dairy Science 69, 413–419.CrossRefGoogle Scholar
Tabaru, H, Ikeda, K, Kadota, E, Murakami, Y, Yamada, H, Sasaki, N and Takeuchi, A (1990) Effects of osmolality on water, electrolytes and VFAs absorption from the isolated ruminoreticulum in the cow. Japanese Journal of Veterinary Science 52, 91–96.Google ScholarPubMed
Tetens, V, Kristensen, NB and Calder, AG (1995) Measurement of 13C enrichment of plasma lactate by gas chromatography/isotope ratio mass spectrometry. Analytical Biochemistry 67, 858–862.Google ScholarPubMed
Weatherburn, MW (1967) Phenol–hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971–973.CrossRefGoogle Scholar
Weekes, TEC and Webster, AJF (1975) Metabolism of butyrate in the tissue of the sheep gut. British Journal of Nutrition 33, 425–438.Google Scholar
Weller, RA, Gray, FV, Pilgrim, AF and Jones, GB (1967) The rates of production of volatile fatty acids in the rumen. IV. Individual and total fatty acids. Australian Journal of Agricultural Research 18, 107–118.Google Scholar