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Distribution of [3H]trans-resveratrol in rat tissues following oral administration

Published online by Cambridge University Press:  08 March 2007

Manal Abd El-Mohsen ,
Henry Bayele ,
Gunter Kuhnle ,
Glenn Gibson ,
Edward Debnam ,
S. Kaila Srai ,
Catherine Rice-Evans  and
Jeremy P. E. Spencer
Manal Abd El-Mohsen
Affiliation:
School of Food Biosciences, University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, UK
Henry Bayele
Affiliation:
Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
Gunter Kuhnle
Affiliation:
Wolfson Centre for Age-related Diseases, GKT School of Biomedical Sciences, King's College, London SE1 9RT, UK
Glenn Gibson
Affiliation:
School of Food Biosciences, University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, UK
Edward Debnam
Affiliation:
Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
S. Kaila Srai
Affiliation:
Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
Catherine Rice-Evans
Affiliation:
Wolfson Centre for Age-related Diseases, GKT School of Biomedical Sciences, King's College, London SE1 9RT, UK
Jeremy P. E. Spencer*
Affiliation:
School of Food Biosciences, University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, UK
*
*Corresponding author: Dr Jeremy P. E. Spencer, fax +44 (0)118 9310080, email [email protected]
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Abstract

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Resveratrol has been widely investigated for its potential health properties, although little is known about its metabolism in vivo Here we investigated the distribution of metabolic products of [3H]trans-resveratrol, following gastric administration. At 2h, plasma concentrations reached 1·7% of the administered dose, whilst liver and kidney concentrations achieved 1·0 and 0·6%, respectively. Concentrations detected at 18h were lower, being only 0·5% in plasma and a total of 0·35% in tissues. Furthermore, whilst kidney and liver concentrations fell to 10 and 25%, respectively, of concentrations at 2h, the brain retained 43% of that measured at 2h. Resveratrol-glucuronide was identified as the major metabolite, reaching 7μm in plasma at 2h. However, at 18h the main form identified in liver, heart, lung and brain was native resveratrol aglycone, indicating that it is the main form retained in the tissues. No phenolic degradation products were detected in urine or tissues, indicating that, unlike flavonoids, resveratrol does not appear to serve as a substrate for colonic microflora. The present study provides additional information about the nature of resveratrol metabolites and which forms might be responsible for its in vivo biological effects.

Keywords

ResveratrolMetabolismPolyphenolRadioactiveDistribution
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 62 - 70
Copyright
Copyright © The Nutrition Society 2006

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