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Digestibility of casein, formaldehyde-treated casein and soya-bean protein in relation to their effects on serum cholesterol in rabbits

Published online by Cambridge University Press:  09 March 2007

Marja W. Kuyvenhoven ,
Wojciech F. Roszkowski ,
Clive E. West ,
Ron L. A. P. Hoogenboom ,
Ria M. E. Vos ,
Anton C. Beynen  and
Roelof Van Der Meer
Marja W. Kuyvenhoven
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands
Wojciech F. Roszkowski
Affiliation:
Institute of Human Nutrition, Agricultural University, Warsaw, Poland
Clive E. West
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands
Ron L. A. P. Hoogenboom
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands
Ria M. E. Vos
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands
Anton C. Beynen
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
Roelof Van Der Meer
Affiliation:
Netherlands Institute for Dairy Research, PO Box 20, 6710 BA Ede, The Netherlands
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Abstract

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Adult male rabbits were fed on semi-purified diets containing soya-bean protein isolate, casein or formaldehyde-treated casein as the protein source and 1 g cholesterol and 5 g of the non-absorbable marker chromic oxide/kg diet. The concentration of cholesterol in serum and in liver was increased on both the casein and formaldehyde-treated-casein diets. Excretion of bile acids and their concentration in faeces were lower in rabbits fed on casein or formaldehyde-treated casein when compared with rabbits fed on soya-bean protein. Apparent digestibility of nitrogen was lowest when formaldehyde-treated casein was fed, and highest on the casein diet. In rabbits fed on casein treated with formaldehyde, higher proportions of N were found in the water-soluble and trichloroacetic acid-insoluble protein fractions of the gastrointestinal tract contents compared with rabbits on the other two diets. Absorption of phosphate from the gastrointestinal tract was higher in rabbits fed on casein than in rabbits fed on soya-bean protein or formaldehyde-treated casein. The results indicate that, in rabbits, protein digestibility may not be an important determinant of serum cholesterol.

Keywords

CholesterolProtein digestibilityRabbit
Type
Lipids
Information
British Journal of Nutrition , Volume 62 , Issue 2 , September 1989 , pp. 331 - 342
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

Beynen, A. C., Van der Meer, R., West, C. E., Sugano, M. & Kritchevsky, D. (1986). Possible mechanisms underlying the differential cholesterolemic effects of dietary casein and soy protein. In Nutritional Effects on Cholesterol Metabolism, pp. 29–45 [Beynen, A. C. editor]. Voorthuizen: Transmondial.Google Scholar
Beynen, A. C., West, C. E., Van Tintelen, G., Van Gils, L. G. M. & Van der Meer, R. (1985). Effect of formaldehyde treatment of dietary casein on serum cholesterol levels in rats. Nutrition Reports International 32, 325–335.Google Scholar
Fiske, C. H. & Subbarow, Y. (1925). The colorimetric determination of phosphorus. Journal of Biological Chemistry 66, 375–400.CrossRefGoogle Scholar
Folch, J., Lees, M. & Stanley, G. H. Sloane (1957). A simple method for the isolation and purification of total lipids from animal sources. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle Scholar
Glatz, J. F. C., Schouten, F. J. M., Engelsman, G. Den & Katan, M. B. (1985). Quantitative determination of neutral steroids and bile acids in human feces by capillary gas–liquid chromatography. In Cholesterol Metabolism in Health and Disease: Studies in the Netherlands, pp. 103–112 [Beynen, A. C.Geelen, M. J. H.Katan, M. B. and Schouten, J. A. editors]. Wageningen: Ponsen & Looijen.Google Scholar
Henry, R. J., Cannon, D. C. & Winkelman, J. W. (1974). Clinical Chemistry: Principles and Technics, pp. 409–411. New York: Harper & Row.Google Scholar
Huff, M. W. & Carroll, K. K. (1980). Effects of dietary protein on turnover, oxidation and absorption of cholesterol, and on steroid excretion in rabbits. Journal of Lipid Research 21, 546–558.CrossRefGoogle ScholarPubMed
Kaufmann, W. & Lüpping, W. (1980). Protected proteins and protected amino acids for ruminants. In Protein Contribution of Foodstuffs for Ruminants: Application to Feed Formulation, pp. 36–75 [Miller, E. L.Pike, I. H. and Van Es, A. J. H. editors]. Boston: Butterworth Scientific.Google Scholar
Kuyvenhoven, M. W., West, C. E., Van der Meer, R. & Beynen, A. C. (1986). Fecal steroid excretion in relation to the development of casein-induced hypercholesterolemia in rabbits. Journal of Nutrition 116, 1395–1404.CrossRefGoogle Scholar
Miller, R. G. Jr (1966). Simultaneous Statistical Influence, pp. 165–172. New York: McGraw-Hill.Google Scholar
Sklan, D., Budowsky, P. & Hurwitz, S. (1979). Absorption of oleic and taurocholic acids from the intestine of the chick. Interactions and interference by proteins. Biochimica et Biophysica Acta 573, 31–39.CrossRefGoogle ScholarPubMed
Van der Meer, R., De Vries, H. & Van Tintelen, G. (1988). The phosphorylation state of casein and the species-dependency of its hypercholesterolaemic effect. British Journal of Nutrition 59, 467–473.CrossRefGoogle ScholarPubMed
Walker, J. F. (1964). Formaldehyde, pp. 399–414. New York: Reinhold Publishing Corporation.Google Scholar
West, C. E., Beynen, A. C., Scholz, K. E., Terpstra, A. H. M., Schutte, J. B., Deuring, K. & Van Gils, L. G. M. (1984). Treatment of dietary casein with formaldehyde reduces its hypercholesterolemic effect in rabbits. Journal of Nutrition 114, 17–25.CrossRefGoogle ScholarPubMed
Williams, C. H., David, D. J. & Iismaa, O. (1962). The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Agricultural Science 59, 381–385.CrossRefGoogle Scholar
Woodward, C. J. H. & Carroll, K. K. (1985). Digestibilities of casein and soya-bean protein in relation to their effects on serum cholesterol in rabbits. British Journal of Nutrition 54, 355–366.CrossRefGoogle ScholarPubMed
Woodward, C. J. H. & West, C. E. (1984). Bile acid binding and the cholesterolaemic effect of dietary proteins. Proceedings of the Nutrition Society 43, 63A.Google Scholar