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Dietary n-6:n-3 fatty acid ratio in the perinatal period affects bone parameters in adult female rats

Published online by Cambridge University Press:  09 March 2007

M. Korotkova ,
C. Ohlsson ,
L. Å. Hanson  and
B. Strandvik
M. Korotkova*
Affiliation:
Department of Pediatrics, Göteborg University, SE 41685 Göteborg, Sweden
C. Ohlsson
Affiliation:
Research Centre for Endocrinology and Metabolism, Department of Internal Medicine, Göteborg University, Göteborg, Sweden
L. Å. Hanson
Affiliation:
Department of Clinical Immunology, Göteborg University, Göteborg, Sweden
B. Strandvik
Affiliation:
Department of Pediatrics, Göteborg University, SE 41685 Göteborg, Sweden
*
*Corresponding author: Dr Marina Korotkova, fax +46 31 21 70 23, email [email protected]
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Abstract

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PUFA and their metabolites are important regulators of bone formation and resorption. The effect of PUFA on bone growth may be especially striking during the perinatal period. The aim of the present study was to investigate the effect of diets with different n-6:n-3 fatty acid (FA) ratios during the perinatal period on bone parameters in the adult offspring. During late gestation and throughout lactation, rat dams were fed an isoenergetic diet containing 70 g linseed oil (n-3 diet), soyabean oil (n-6+n-3 diet) or sunflower-seed oil (n-6 diet) per kg with n-6:n-3 FA ratios of 0·4, 9 and 216, respectively. The offspring were weaned onto an ordinary chow and followed until 30 weeks of age. Bone parameters were analysed using peripheral quantitative computerised tomography and dual-energy X-ray absorptiometry. Femur length and cortical cross-sectional bone area and bone mineral content were significantly higher in the n-6+n-3 group than in the other groups. Cortical bone thickness in the n-6+n-3 group was increased compared with the n-3 group, but most cortical bone parameters did not differ between the n-3 and n-6 groups. The results suggest that regulatory mechanisms were influenced by the n-6:n-3 FA ratio early in life and not compensated for by the introduction of an ordinary diet after weaning.

Keywords

DietBone mineral densityFatty acidsInsulin-like growth factor-1
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 4 , October 2004 , pp. 643 - 648
Copyright
Copyright © The Nutrition Society 2004

References

Chlebna-Sokol, D & Rusinska, A (2001) Serum insulin/like growth factor I, bone mineral density and biochemical markers of bone metabolism in children with idiopathic osteoporosis. Endocr Regul 35, 201–208.Google ScholarPubMed
Folch, J, Lees, M, Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497–509.CrossRefGoogle ScholarPubMed
Gordeladze, JO, Drevon, CA, Syversen, U & Reseland, JE (2002) Leptin stimulates human osteoblastic cell proliferation, de novo collagen synthesis, and mineralization: impact on differentiation markers, apoptosis, and osteoclastic signaling. J Cell Biochem 85, 825–836.CrossRefGoogle ScholarPubMed
Innis, SM & Kuhnlein, HV (1988) Long-chain n -3 fatty acids in breast milk of Inuit women consuming traditional foods. Early Hum Dev 18, 185–189.CrossRefGoogle ScholarPubMed
Iwami-Morimoto, Y, Yamaguchi, K & Tanne, K (1999) Influence of dietary n -3 polyunsaturated fatty acid on experimental tooth movement in rats. Angle Orthod 69, 365–371.Google ScholarPubMed
Jones, G, Riley, MD & Dwyer, T (2000) Maternal diet during pregnancy is associated with bone mineral density in children: a longitudinal study. Eur J Clin Nutr 54, 749–756.CrossRefGoogle ScholarPubMed
Judex, S, Wohl, GR, Wolff, RB, Leng, W, Gillis, AM & Zernicke, RF (2000) Dietary fish oil supplementation adversely affects cortical bone morphology and biomechanics in growing rabbits. Calcif Tissue Int 66, 443–448.Google Scholar
Knapp, HR (1990) Prostaglandins in human semen during fish oil ingestion: evidence for in vivo cyclooxygenase inhibition and appearance of novel trienoic compounds. Prostaglandins 39, 407–423.CrossRefGoogle ScholarPubMed
Kokkinos, PP, Shaye, R, Alam, BS & Alam, SQ (1993) Dietary lipids, prostaglandin E2 levels, and tooth movement in alveolar bone of rats. Calcif Tissue Int 53, 333–337.Google Scholar
Korotkova, M, Gabrielsson, B, Hanson, LA & Strandvik, B (2001) Maternal essential fatty acid deficiency depresses serum leptin levels in suckling rat pups. J Lipid Res 42, 359–365.CrossRefGoogle ScholarPubMed
Korotkova, M, Gabrielsson, B, Lonn, M, Hanson, LA & Strandvik, B (2002) Leptin levels in rat offspring are modified by the ratio of linoleic to alpha-linolenic acid in the maternal diet. J Lipid Res 43, 1743–1749.CrossRefGoogle ScholarPubMed
Li, Y, Seifert, MF, Ney, DM, Grahn, M, Grant, AL, Allen, KG & Watkins, BA (1999) Dietary conjugated linoleic acids alter serum IGF-I and IGF binding protein concentrations and reduce bone formation in rats fed ( n -6) or ( n -3) fatty acids. J Bone Miner Res 14, 1153–1162.CrossRefGoogle ScholarPubMed
Lucas, A (1991) Programming by early nutrition in man. Ciba Found Symp 156, 38–50.Google Scholar
Lucas, A (1998) Programming by early nutrition: an experimental approach. J Nutr 128, 401S–406S.CrossRefGoogle ScholarPubMed
McCarthy, TL, Centrella, M, Raisz, LG & Canalis, E (1991) Prostaglandin E2 stimulates insulin-like growth factor I synthesis in osteoblast-enriched cultures from fetal rat bone. Endocrinology 128, 2895–2900.Google Scholar
Mehta, G, Roach, HI, Langley-Evans, S, Taylor, P, Reading, I, Oreffo, RO, Aihie-Sayer, A, Clarke, NM & Cooper, C (2002) Intrauterine exposure to a maternal low protein diet reduces adult bone mass and alters growth plate morphology in rats. Calcif Tissue Int 71, 493–498.CrossRefGoogle ScholarPubMed
Moreira-Andres, MN, Papapietro, K, Canizo, FJ, Rejas, J, Larrodera, L & Hawkins, FG (1995) Correlations between bone mineral density, insulin-like growth factor I and auxological variables. Eur J Endocrinol 132, 573–579.CrossRefGoogle ScholarPubMed
Ohlsson, C, Bengtsson, BA, Isaksson, OG, Andreassen, TT & Slootweg, MC (1998) Growth hormone and bone. Endocr Rev 19, 55–79.Google ScholarPubMed
Okada, Y, Lorenzo, JA, Freeman, AM, Tomita, M, Morham, SG, Raisz, LG & Pilbeam, CC (2000) Prostaglandin G/H synthase-2 is required for maximal formation of osteoclast-like cells in culture. J Clin Invest 105, 823–832.CrossRefGoogle Scholar
Raisz, LG & Fall, PM (1990) Biphasic effects of prostaglandin E2 on bone formation in cultured fetal rat calvariae: interaction with cortisol. Endocrinology 126, 1654–1659.CrossRefGoogle Scholar
Rocquelin, G, Kiffer, J, Tapsoba, S & Bouda, C (2001) High proportions of n -6 polyunsaturated fatty acids in mature milk of mothers in Ouagadougou, Burkina Faso. Acta Paediatr 90, 450–452.Google Scholar
Sakaguchi, K, Morita, I & Murota, S (1994) Eicosapentaenoic acid inhibits bone loss due to ovariectomy in rats. Prostaglandins Leukot Essent Fatty Acids 50, 81–84.CrossRefGoogle ScholarPubMed
Sjogren, K, Sheng, M, Moverare, S, Liu, JL, Wallenius, K, Tornell, J, Isaksson, O, Jansson, JO, Mohan, S & Ohlsson, C (2002) Effects of liver-derived insulin-like growth factor I on bone metabolism in mice. J Bone Miner Res 17, 1977–1987.CrossRefGoogle Scholar
Steppan, CM, Crawford, DT, Chidsey-Frink, KL, Ke, H & Swick, AG (2000) Leptin is a potent stimulator of bone growth in ob/ob mice. Regul Pept 92, 73–78.CrossRefGoogle ScholarPubMed
Takiguchi, T, Kobayashi, M, Nagashima, C, Yamaguchi, A, Nishihara, T & Hasegawa, K (1999) Effect of prostaglandin E2 on recombinant human bone morphogenetic protein-2-stimulated osteoblastic differentiation in human periodontal ligament cells. J Periodontal Res 34, 431–436.Google Scholar
Vidal, O, Lindberg, MK, Hollberg, K, Baylink, DJ, Andersson, G, Lubahn, DB, Mohan, S, Gustafsson, JA & Ohlsson, C (2000) Estrogen receptor specificity in the regulation of skeletal growth and maturation in male mice. Proc Natl Acad Sci USA 97, 5474–5479.Google Scholar
Watkins, BA, Li, Y, Allen, KG, Hoffmann, WE & Seifert, MF (2000) Dietary ratio of ( n -6)/( n -3) polyunsaturated fatty acids alters the fatty acid composition of bone compartments and biomarkers of bone formation in rats. J Nutr 130, 2274–2284.CrossRefGoogle Scholar
Watkins, BA, Li, Y, Lippman, HE & Seifert, MF (2001) Omega-3 polyunsaturated fatty acids and skeletal health. Exp Biol Med (Maywood) 226, 485–497.CrossRefGoogle ScholarPubMed
Watkins, BA, Shen, CL, Allen, KG & Seifert, MF (1996) Dietary ( n -3) and ( n -6) polyunsaturates and acetylsalicylic acid alter ex vivo PGE2 biosynthesis, tissue IGF-I levels, and bone morphometry in chicks. J Bone Miner Res 11, 1321–1332.CrossRefGoogle ScholarPubMed
Watkins, BA, Shen, CL, McMurtry, JP, Xu, H, Bain, SD, Allen, KG & Seifert, MF (1997) Dietary lipids modulate bone prostaglandin E2 production, insulin-like growth factor-I concentration and formation rate in chicks. J Nutr 127, 1084–1091.CrossRefGoogle ScholarPubMed
Windahl, SH, Vidal, O, Andersson, G, Gustafsson, JA & Ohlsson, C (1999) Increased cortical bone mineral content but unchanged trabecular bone mineral density in female ERbeta(-/-) mice. J Clin Invest 104, 895–901.CrossRefGoogle ScholarPubMed