Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-28T12:48:27.123Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary conjugated linoleic acids promote fatty streak formation in the C57BL/6 mouse atherosclerosis model

Published online by Cambridge University Press:  09 March 2007

John S. Munday ,
Keith G. Thompson  and
Kerry A. C. James
John S. Munday*
Affiliation:
Institute of Veterinary, Animal and Biomedical Sciences Massey University, Palmerston North, New Zealand
Keith G. Thompson
Affiliation:
Institute of Veterinary, Animal and Biomedical Sciences Massey University, Palmerston North, New Zealand
Kerry A. C. James
Affiliation:
New Zealand Institute for Crop and Food Research Limited, Palmerston North, New Zealand
*
*Corresponding author: Dr John S. Munday, fax +646 350 2270, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Conjugated linoleic acids (CLA) are positional isomers of linoleic acid which have been suggested by some to possess antiatherosclerotic properties. To test this hypothesis, three groups of twenty C57BL/6 mice were fed on atherogenic diets containing: 5 g CLA/kg, 2·5 g CLA + 2·5 g linoleic acid/kg or 5 g linoleic acid/kg. All diets were fed for 15 weeks and contained (g/kg): triacylglycerol 145, free fatty acids 5, cholesterol 10 and cholic acid 5. At the completion of the experimental period, when data from both groups fed on CLA were combined, dietary CLA did not produce significant differences in body weight, serum total cholesterol concentration or serum HDL-cholesterol concentration. However, mice receiving CLA developed a significantly higher serum HDL-cholesterol: total cholesterol ratio and a significantly lower serum triacylglycerol concentration than controls. Despite causing a serum lipoprotein profile considered to be less atherogenic, the addition of CLA to the atherogenic diet increased the development of aortic fatty streaks. Considering the increased atherogenesis associated with dietary CLA in the present study, and the failure to demonstrate a significant beneficial effect of CLA in other animal studies, there is currently no conclusive evidence to support the hypothesis that CLA protect against atherogenesis.

Keywords

AtherosclerosisConjugated linoleic acidsDietary fat
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 3 , March 1999 , pp. 251 - 255
Copyright
Copyright © The Nutrition Society 1999

References

Aviram, M (1996) Interaction of oxidised low density lipoprotein with macrophages in atherosclerosis, and the antiatherogenicity of antioxidants. European Journal of Clinical Chemistry and Clinical Biochemistry 34, 599–608.Google Scholar
Belury, MA & Kempa-Steczko, A (1997) Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32, 199–204.Google Scholar
Breslow, J, Plump, A & Dammerman, M (1996) New mouse models of lipoprotein disorders and atherosclerosis. In Atherosclerosis and Coronary Artery Disease, pp. 363–367 [Fuster, V, Ross, R and Topol, EP, editors]. Philadelphia, PA: Lippincott-Raven Publishers.Google Scholar
Chin, SF, Liu, W, Storkson, JM & Pariza, MW (1992) Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognised class of anticarcinogens. Journal of Food Composition and Analysis 5, 185–197.CrossRefGoogle Scholar
Cook, ME, Miller, CC, Park, Y & Pariza, M (1993) Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poultry Science 72, 1301–1305.CrossRefGoogle ScholarPubMed
Fuster, V (1994) Mechanisms leading to myocardial infarction: insights from studies of vascular biology (Lewis A. Conner Memorial Lecture). Circulation 90, 2126–2146.Google Scholar
Ha, YL, Storkson, JM & Pariza, MW (1990) Inhibition of benz(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Research 50, 1097–1101.Google Scholar
Haumann, BF (1996) Conjugated linoleic acid. International News on Fats, Oils and Related Materials 7, 152–159.Google Scholar
Ip, C, Singh, M, Thompson, HJ & Scimeca, JA (1991) Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Research 51, 6118–6124.Google Scholar
Izzo, C, Grillo, F & Murador, E (1981) Improved method for determination of high-density-lipoprotein cholesterol I. Isolation of high-density lipoproteins by use of polyethylene glycol 6000. Clinical Chemistry 27, 371–374.CrossRefGoogle ScholarPubMed
Lee, KN, Kritchevsky, D & Pariza, MW (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19–25.CrossRefGoogle ScholarPubMed
Luria, MH, Erel, J, Sapoznikov, D & Gotsman, MS (1991) Cardiovascular risk factor clustering and ratio of total cholesterol to high-density lipoprotein cholesterol in angiographically documented coronary artery disease. American Journal of Cardiology 67, 31–36.Google Scholar
Miller, CC, Park, Y, Pariza, MW & Cook, ME (1994) Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochemical and Biophysical Research Communications 198, 1107–1112.CrossRefGoogle ScholarPubMed
Munday, JS, Thompson, KG, James, KAC & Manktelow, BW (1998) Dietary antioxidants do not reduce fatty streak formation in the C57BL/6 mouse atherosclerosis model. Arteriosclerosis, Thrombosis, and Vascular Biology 18, 114–119.Google Scholar
National Research Council (1995) Nutrient Requirements of Laboratory Animals, 4th revised ed. Washington, DC: National Academy Press.Google Scholar
Nicolosi, RJ, Rogers, EJ, Kritchevsky, D, Scimeca, JA & Huth, PJ (1997) Dietary conjugated linoleic acid reduces plasma lipoproteins and early atherosclerosis in hypercholesterolemic hamsters. Artery 22, 266–277.Google Scholar
Nishina, PM, Lowe, S, Verstuyft, J, Naggert, JK, Kuypers, FA & Paigen, B (1993) Effects of dietary fats from animal and plant sources on diet-induced fatty streak lesions in C57BL/6J mice. Journal of Lipid Research 34, 1413–1422.CrossRefGoogle ScholarPubMed
Nishina, PM, Verstuyft, J & Paigen, B (1990) Synthetic low and high fat diets for the study of atherosclerosis in the mouse. Journal of Lipid Research 31, 859–869.CrossRefGoogle Scholar
Paigen, B, Holmes, PA, Mitchell, D & Albee, D (1987 a) Comparison of atherosclerotic lesions and HDL-lipid levels in male, female, and testosterone-treated female mice from strains C56BL/6, BALB/C and C3H. Atherosclerosis 64, 215–221.Google Scholar
Paigen, B, Morrow, A, Holmes, P, Mitchell, D & Williams, R (1987 b) Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis 68, 231–240.Google Scholar
Park, Y, Albright, KJ, Liu, W, Storkson, JM, Cook, ME & Pariza, MW (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32, 853–858.CrossRefGoogle ScholarPubMed
Parodi, PW (1997) Cows' milk fat components as potential anticarcinogenic agents. Journal of Nutrition 127, 1055–1060.Google Scholar
Stampfer, MJ, Sacks, FM, Salvini, S, Willett, WC & Henekens, CH (1991) A prospective study of cholesterol, apolipoproteins and risk of myocardial infarction. New England Journal of Medicine 325, 373–381.Google Scholar
Stewart-Phillips, JL & Lough, J (1991) Pathology of atherosclerosis in cholesterol-fed, susceptible mice. Atherosclerosis 90, 211–218.CrossRefGoogle ScholarPubMed
van den Berg, JJM, Cook, NE & Tribble, DL (1995) Reinvestigation of the antioxidant properties of conjugated linoleic acid. Lipids 30, 599–605.CrossRefGoogle ScholarPubMed
Williams, KJ & Tabas, I (1995) The response-to-retention hypothesis of early atherogenesis. Arteriosclerosis, Thrombosis, and Vascular Biology 15, 551–561.Google Scholar
Wong, MW, Chew, BP, Wong, TS, Hosick, HL, Boylston, TD & Shultz, TD (1997) Effects of dietary conjugated linoleic acid on lymphocyte function and growth of mammary tumors in mice. Anticancer Research 17, 987–994.Google Scholar