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The contribution of protozoa to the protein entering the duodenum of sheep

Published online by Cambridge University Press:  09 December 2008

D. G. Harrison ,
D. E. Beever  and
D. F. Osbourn
D. G. Harrison
Affiliation:
Department of Agricultural Biochemistry, University of Newcastle upon Tyne, Newcastle upon Tyne NEI 7RU
D. E. Beever
Affiliation:
The Grassland Research Institute, Hurley, Maidenhead, Berkshire SL6 5LR
D. F. Osbourn
Affiliation:
The Grassland Research Institute, Hurley, Maidenhead, Berkshire SL6 5LR
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Abstract

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1. Four sheep, each fitted with a rumen fistula and a re-entrant cannula at the proximal duodenum were fed a semi-purified diet containing urea as the only nitrogen source. The quantities of total protozoal amino acid-N (TPAN) present in the rumen and entering the duodenum were determined when the mean rumen dilution rate (D) was low (0.034/h) and when D was increased to 0.078/h by the intraruminal infusion of artificial saliva.

2. Increasing the dilution rate had no significant effect upon the proportions of TPAN present in the total microbial amino acid-N (TMAN) of the rumen fluid and duodenal digesta. With both dilution rates the mean proportion of TPAN in the duodenal TMAN (0.24) was markedly less than the equivalent proportion (0.45) found in the rumen fluid.

3. The daily flow of TPAN, as measured at the duodenal cannula at both dilution rates was equivalent to only 41 % of the flow of TPAN as predicted from measurements of rumen outflow, indicating that a substantial proportion of rumen protozoal protein was retained within the rumen.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 41 , Issue 3 , May 1979 , pp. 521 - 527
Copyright
Copyright © The Nutrition Society 1979

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