Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-08T14:29:49.971Z Has data issue: false hasContentIssue false
  • English
  • Français

Conjunctival impression cytology with transfer (CICT) to detect pre-clinical vitamin A deficiency among slum children in India

Published online by Cambridge University Press:  09 March 2007

Swapan Chowdhury ,
Rajesh Kumar ,
N. K. Ganguly ,
Lata Kumar ,
C. K. Nain  and
B. N. S. Walia
Swapan Chowdhury
Affiliation:
Deparment of Experimental MedicinePostgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Rajesh Kumar
Affiliation:
Departments of Community Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
N. K. Ganguly
Affiliation:
Deparment of Experimental MedicinePostgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Lata Kumar
Affiliation:
Department of PaediatricsPostgraduate Institute of Medical Education and Research, Chandigarh-160012,India
C. K. Nain
Affiliation:
Deparment of Experimental MedicinePostgraduate Institute of Medical Education and Research, Chandigarh-160012, India
B. N. S. Walia
Affiliation:
Department of PaediatricsPostgraduate Institute of Medical Education and Research, Chandigarh-160012,India
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In order to establish a method usable in the field for diagnosis of pre-clinical vitamin A deficiency, conjunctival impression cytology with transfer (CICT) was used in 200normal slum children aged 6−120 months in Chandigarh, India. Conjunctival impressions taken on cellulose acetate paper were transferred to glass slides which were fixed in ethanol and stained with alcian blue and carbol fuchsin. Sixty samples of cellulose acetate paper were preserved after transfer of impression and were stained later. Transfer was complete in forty-eight samples and in the other twelve it was incomplete. Conjunctival impressions on paper and slide of these twelve cases were comparable. At a plasma retinol concentration of <0·70 pmol/l measured by HPLC the sensitivity, specificity and positive predictive values of CICT were 90·5%, 100% and 100% respectively. Compared with conventional conjunctival impression cytology, CICT is less time consuming, cheaper and comparable in validity. It is thus more suitable than the conventional method for mass screening

Keywords

Conjunctival impression cytology with transferVitamin ARetinol
Type
Detction of pre-clinical vitamin A deficiency
Information
British Journal of Nutrition , Volume 75 , Issue 5 , May 1996 , pp. 785 - 790
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Carlier, C., Moulia-Pelat, J. P., Ceccon, J. F., Mourey, M. S., Fall, M., Diaye, M. N. & Amedee-Manesme, O. (1991 a). Prevalence of malnutrition and vitamin A deficiency in the Diourbel, Fatick and Kaolack Regions of Senegal: feasibility of the method of impression cytology with transfer. American Journal of Clinical Nutrition 53, 66–69.CrossRefGoogle ScholarPubMed
Carlier, C., Moulia-Pelat, J. P., Ceccon, J. F., Mourey, M. S., Malvy, D., Fall, M., Diaye, M. N.Amedee-Manesme, O. (1991 b). Prevalence of malnutrition and vitamin A deficiency in the Diurbel, Fatick, and Kaolack regions of Senegal: a controlled study. American Journal of Clinical Nutrition 53, 74–77.CrossRefGoogle ScholarPubMed
Gadomski, A. M., Kjolhede, C. L., Wittpenn, J., Rosas, A. R. & Forman, M. R. (1989). Conjunctival impression cytology (CIC) to detect sub-clinical vitamin A deficiency: comparison of CIC with biochemical assessments. American Journal of Clinical Nutrition 49, 495–500.CrossRefGoogle Scholar
Humphrey, J. H., West, K. P. Jr & Sommer, A. (1992). Vitamin A deficiency and attributable mortality among under-5-year-olds. Bulletin of the World Health Organization 70, 225–232.Google ScholarPubMed
Indian Council of Medical Research (1974). Studies in preschool children. ICMR Technical Report Series 26, 15–19.Google Scholar
Luzeau, R., Carlier, C., Ellrodt, A. & Amedee-Manesme, O. (1988). Impression cytology with transfer: an easy method for detection of vitamin A deficiency. International Journal for Vitamin and Nutrition Research 58, 166–170.Google ScholarPubMed
Natadisastra, G., Wittpenn, J. R., Muhilal, West K. P., Mele, L. & Sommer, A. (1988). Impression cytology: a practical index of vitamin A status. American Journal of Clinical Nutrition 48, 695–701.CrossRefGoogle ScholarPubMed
Olson, J. A. (1984). Serum levels of vitamin A and carotenoids as reflectors of nutritional status. Journal of the National Cancer Institute 73, 1439–1444.Google ScholarPubMed
Pilch, S. M. (1987). Analysis of vitamin A data from the health and nutrition examination surveys. Journal of Nutrition 117, 636–640.CrossRefGoogle ScholarPubMed
Reddy, V., Rao, V. Arunjyothi, & Reddy, M. (1989). Conjunctival impression cytology for assessment of vitamin A status. American Journal of Clinical Nutrition 50, 814–817.CrossRefGoogle ScholarPubMed
Sommer, A. (1989). New imperative for an old vitamin (A). Journal ofNutrition 119, 96–100.Google ScholarPubMed
Thurnham, D., Smith, E. & Flora, P. S. (1988). Concurrent liquid-chromatographic assay of retinol, α-tocopherol, β-carotene, α-carotene, lycopene and β-cryptoxanthin in plasma with tocopherol acetate as internal standard. Clinical Chemistry 34, 377–381.CrossRefGoogle ScholarPubMed
Wittpenn, J. R., Tseng, S. C. G. & Sommer, A. (1986). Detection of early xerophthalmia by impression cytology. Archives of Ophthalmology 104, 237–239.CrossRefGoogle ScholarPubMed
World Health Organization (1982). Control of vitamin A deficiency and xerophthalmia, pp. 7–20. WHO Technical Report Series no. 672. Geneva: World Health Organization.Google Scholar