Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-14T21:24:33.155Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of exogenous glucose, fructose and galactose oxidation during exercise using 13C-labelling

Published online by Cambridge University Press:  08 March 2007

Yan Burelle ,
Marie-Catherine Lamoureux ,
François Pèronnet ,
Denis Massicotte  and
Carole Lavoie
Yan Burelle
Affiliation:
Department of Kinesiology, University of Montreal, CP 6128 Centre Ville, Montreal, Quebec, H3C 3J7, Canada
Marie-Catherine Lamoureux
Affiliation:
Department of Kinesiology, University of Montreal, CP 6128 Centre Ville, Montreal, Quebec, H3C 3J7, Canada
François Pèronnet
Affiliation:
Department of Kinesiology, University of Montreal, CP 6128 Centre Ville, Montreal, Quebec, H3C 3J7, Canada
Denis Massicotte*
Affiliation:
Department of Kinanthropology, University of Quebec at Montreal, Montreal, Quebec, H3C 3P8, Canada
Carole Lavoie
Affiliation:
Department of Science of Physical Activity, University of Quebec at Trois-Riviéres, Quebec, G9A 5H7, Canada
*
*Corresponding author: Dr Denis Massicotte, fax +1 514 987 6616, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Six subjects exercised for 120;min on a cycle ergometer (65 (se 3) % V˙O2max) when ingesting a placebo or glucose, fructose or galactose (100g in 1000 ml water) labelled with 13C. The oxidation of energy substrates including exogenous hexoses was compared using indirect respiratory calorimetry and 13CO2 production at the mouth. Total carbohydrate progressively decreased and total fat oxidation increased over the 120min exercise period in the four experimental situations. During the 120min of exercise, the amount of fructose oxidized (38·8 (se 2·6) g; 9·0 (se 0·6)% energy yield) was not significantly (approximately 4%) lower than that of exogenous glucose (40·5 (se 3·4) g; 9·2 (se 0·8)% energy yield), while that of galactose (23·7 (se 3·5) g; 5·5 (se 0·9) % energy yield) was only 59% and 61% that of glucose and fructose, respectively. When compared with the placebo, the ingestion and oxidation of the three hexoses did not significantly modify fat oxidation or total carbohydrate oxidation, but it significantly reduced (9–13%) endogenous carbohydrate oxidation. The present data indicate that fructose and exogenous glucose ingested during exercise could be oxidized at a similar rate, but that the oxidation rate of galactose was only approximately 60% that of the exogenous glucose and fructose, presumably because of a preferential incorporation of galactose into liver glycogen (Leloir pathway). The reduction in endogenous carbohydrate oxidation was, however, similar with the three hexoses.

Keywords

Stable isotopeExogenous hexosesCalorimetry
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 56 - 61
Copyright
Copyright © The Nutrition Society 2006

References

Adopo, E, Péronnet, F, Massicotte, DBrisson, GR & Hillaire-Marcel, CRespective oxidation of exogenous glucose and fructose given in the same drink during exercise. J Appl Physiol (1994) 76, 1014–1019.CrossRefGoogle ScholarPubMed
Ahlborg, G & Björkman, OSplanchnic and muscle fructose metabolism during and after exercise. J Appl Physiol (1990) 69, 1244–1251.CrossRefGoogle ScholarPubMed
Berry, GT, Nissim, I, Mazur, AT, Elsas, LJ, Singh, RH, Klein, PD, Gibson, JB, Lin, z & Segal, SIn vivo oxidation of [13C] galactose in patients with galactose-1-phosphate uridyltransferase deficiency. Biochem Mol Med (1995) 56, 158–165.CrossRefGoogle ScholarPubMed
Burelle, Y, Massicotte, DLussier, M, Lavoie, C, Hillaire-Marcel, C & Péronnet, FOxidation of (13C) glycerol ingested along with glucose during prolonged exercise. J Appl Physiol (2001) 90, 1685–1690.CrossRefGoogle ScholarPubMed
Burelle, Y, Péronnet, F, Massicotte, D, Brisson, & Hillaire-Marcel, Cxidation of 13C-glucose and 13C-fructose ingested as a pre-exercise meal: effect of carbohydrate ingestion during exercise. Int J Sports Nutr (1997) 7, 117–127.CrossRefGoogle Scholar
Chen, M & Whistler, RLMetabolism of D-fructose. Adv Carb Chem Biochem (1977) 34, 285–343.Google ScholarPubMed
Couture, S, Massicotte, D, Lavoie, c, Hillaire-Marcel, c & Péronnet, FOral 13C-glucose and endogenous energy substrate oxidation during prolonged treadmill running. J Appl Physiol (2002) 92, 1255–1260.CrossRefGoogle Scholar
Craig, HThe geochemistry of stable isotopes. Geochem Cosmochem Acta (1953) 3, 53–92.CrossRefGoogle Scholar
Décombaz, J, Sartori, D, Arnaud, MJ, Thélin, AL, Schürch, P & Howald, HOxidation and metabolic effects of fructose and glucose ingested before exercise. Int J Sports (1985) 6, 282–286.CrossRefGoogle ScholarPubMed
Ferraris, RP & Diamond, JRegulation of intestinal sugar transport. Physiol Rev (1997) 77, 257–302.CrossRefGoogle ScholarPubMed
Ganda, OP, Soldner, JS, Gleason, RE & Cleator, IGMMetabolic effect of glucose, mannose, galactose, and fructose in man. J Clin Endocrinol Metab (1979) 49, 616–622.CrossRefGoogle Scholar
Guézennec, CY, Satabin, P, Duforez, F, Merino, D, Péronnet, F & Koziet, JOxidation of corn starch, glucose, and fructose ingested before exercise. Med Sci Sports Exerc (1989) 21, 45–50.CrossRefGoogle ScholarPubMed
Jandrain, BJ, Pallikarakis, N, Normand, S et al. . Fructose utilization during exercise in men: rapid conversion of ingested fructose to circulating glucose. J Appl Physiol (1993) 74, 2146–2154.CrossRefGoogle ScholarPubMed
Jentjens, RLP, Achten, J & Jeukendrup, AEHigh oxidation rates from combined carbohydrates ingested during exercise. Med Sci Sports Exerc (2004a) 36, 1551–1558.CrossRefGoogle ScholarPubMed
Jentjens, RLP, Venables, MC & Jeukendrup, AEOxidation of exogenous glucose, sucrose, and maltose during prolonged cycling exercise. J Appl Physiol (2004b) 96, 1285–1291.CrossRefGoogle ScholarPubMed
Jeukendrup, AECarbohydrate intake during exercise and performance. Nutrition (2004) 20, 669–677.CrossRefGoogle ScholarPubMed
Jeukendrup, AE, Wagenmakers, AJM, Stegen, JHCH, Gijsen, AP, Brouns, F & Saris, WHMCarbohydrate ingestion can completely suppress endogenous glucose production during exercise. Am J Physiol (1999) 276, E672–E683.Google ScholarPubMed
Kellett, GLThe facilitated component of intestinal glucose absorption. Topical review. J Physiol (2001) 531, 585–595.CrossRefGoogle Scholar
Lefe`bvre, PJFrom plant physiology to human metabolic investigations. Diabetologia (1985) 28, 255–263.CrossRefGoogle Scholar
Leijssen, DPC,Saris, WHM, Jeukendrup, AE & Wagenmakers, AJMOxidation of exogenous [13C]galactose and [13C]glucose during exercise. J Appl Physiol (1995) 79, 720–725.CrossRefGoogle ScholarPubMed
Massicotte, D, Péronnet, F, Adopo, E, Brisson, GR & Hillaire-Marcel, CEffect of metabolic rate on the oxidation of ingestedglucose and fructose during exercise. Int J Sports Med (1994) 15, 177–180.CrossRefGoogle ScholarPubMed
Massicotte, d, Péronnet, F, Allah, C, Hillaire-Marcel, C, Ledoux, M & Brisson, GMetabolic response to [13C]glucose and [13C]fructose ingestion during exercise. J Appl Physiol (1986) 61, 1180–1184.CrossRefGoogle ScholarPubMed
Massicotte, DPéronnet, F, Brisson, G, Bakkouch, K & Hillaire-Marcel, COxidation of a glucose polymer during exercise: comparison with glucose and fructose. J Appl Physiol (1989) 66, 179–183.CrossRefGoogle ScholarPubMed
Massicotte, D, Péronnet, F, Brisson, G, Boivin, L & Hillaire-Marcel, COxidation of exogenous carbohydrate during prolonged exercise in fed and fasted conditions. Int J Sports Med (1990) 11, 253–258.CrossRefGoogle ScholarPubMed
Pallikarakis, N, Sphiris, N & Lefe`bvre, PInfluence of the bicarbonate pool on the occurrence of 13CO2 in exhaled air. Eur J Appl Physiol (1991) 63, 179–183.CrossRefGoogle ScholarPubMed
Pereira, LO & Lancha, AHEffect of insulin and contraction up on glucose transport in skeletal muscle. Review. Prog Biophys Mol Biol (2004) 84, 1–27.CrossRefGoogle Scholar
Péronnet, F & Massicotte, DTable of non protein respiratory quotient: an update. Can J Sport Sci (1991) 16, 23–29.Google Scholar
Pirnay, F, Sheen, AJ, Gautier, JF, Lacroix, M, Mosora, F & Lefébvre, PJExogenous glucose oxidation during exercise in relation to the power output. Int J Sports Med (1995) 16, 456–460.Google Scholar
Ruzzin, J, Péronnet, F, Tremblay, J, Massicotte, D & Lavoie, CBreath [13CO2] recovery from an oral glucose load during exercise: comparison between[U-13C] and [1, 2-13C]glucose. J Appl Physiol (2003) 95, 477–482.CrossRefGoogle Scholar
Santer, R, Hillebrand, G, Steinmann, B & Schaub, JIntestinal glucose transporter: evidence for a membrane traffic-base pathway in humans. Gastroenterology (2003) 124, 34–39.CrossRefGoogle ScholarPubMed
Slama, G, Boillot, J, Hellal, I, Darmaun, D, Rizkalla, SW, Orvoen-Friza, E, Doré, MF, Guille, G, Fretault, J & Coursaget, JFructose is as good a fuel as glucose for exercise in normal subjects. Diabet Metabol (Paris) (1989) 15, 105–106.Google Scholar
Stellaard, F, Koetse, HA, Elzinga, H, Boverhof, R, Tjoonk, R, Klimp, A, Vegter, D, Liesker, J & Vonk, RJ13C-carbohydrate breath test: impact of physical activity on the rate-limiting step in lactose utilization. Scand J Gastroenterol (2000) 35, 819–823.Google ScholarPubMed
Stümpel, F, Burcelin, R, Jungermann, K & Thorens, Normal kinetics of intestinal glucose absorption in the absence of GLUT2: evidence for a glucose pathway requiring glucose phosphorylation and transfer into the endoplasmic Reticul. Proc Nat Acad Sci (USA) (2001) 98, 11330–11335.CrossRefGoogle Scholar
Trimmer, JK,Casazza, GA, Horning, MA & Brooks, GARecovery of 13CO2 during rest and exercise after [1-13C]acetate, [2–13C]acetate, and NaH13CO3 infusions. Am J Physiol (2001) 281, E683–E692.Google Scholar
Wagenmakers, AJM, Brouns, F & Saris, WHMOxidation rate of orally ingested carbohydrates during prolonged exercise in man. J Appl Physiol (1993) 75, 2774–2780.CrossRefGoogle Scholar
Wallis, GA, Rowlands, DS, Shaw, C, Jentjens, RL & Jeukendrup, AEOxidation of combined ingestion of maltodextrins and fructose during exercise. Med Sci Sports Exerc (2005) 37, 426–432.CrossRefGoogle ScholarPubMed
Williams, CAMetabolism of lactose and galactose in man. Prog Biochem Pharmacol (1986) 21, 219–247.Google ScholarPubMed
Wright, EM, Martin, GM & Turk, EIntestinal absorption in health and disease – sugars. Best Pract Res Clin Gastroenterol (2003) 17, 943–956.CrossRefGoogle ScholarPubMed