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Biotin studies in pigs

5. The post-ileal absorption of biotin

Published online by Cambridge University Press:  09 March 2007

J. S. Kopinski ,
Jane Leibholz ,
Jane Leibholz  and
R.J. Love
J. S. Kopinski
Affiliation:
Department of Animal Husbandry, University of Sydney, Camden, New South Wales 2570, Australia
Jane Leibholz
Affiliation:
Department of Animal Husbandry, University of Sydney, Camden, New South Wales 2570, Australia
Jane Leibholz
Affiliation:
Department of Animal Husbandry, University of Sydney, Camden, New South Wales 2570, Australia
R.J. Love
Affiliation:
Department of Animal Husbandry, University of Sydney, Camden, New South Wales 2570, Australia
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Abstract

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Six pigs were given a biotin-deficient diet (10 μg biotin/kg) for 14 weeks. The pigs were given the same diet for a further 42 d with one of three biotin treatments: no supplement, 400 μg biotin/d given orally, 400 μg biotin/d infused into the caecum. The concentrations of biotin in the liver, kidney and heart, at the conclusion of 42 d were greater in the pigs given the oral biotin supplement than in the unsupplemented pigs. The values for the pigs given the caecal infusion of biotin were intermediate. The excretion of biotin in faeces was increased from 35 to 101 μg/d by the caecal biotin infusion, and the urinary excretion was increased from 35 to 83 μg/d. The oral dose of biotin increased urinary biotin excretion from 35 to 345 μg/d, but there was no change in faecal biotin excretion. Two pigs were fitted with ileal cannulas and catheters in the vena cava. The pigs were given a dose of [14C] biotin either into the caecum, when the ileum was occluded by a bladder catheter, or orally. [14C] biotin was measured in the urine and plasma and showed that post-ileal biotin absorption was 8 % as efficient as the absorption of biotin after oral dosing.

Keywords

Biotin absorptionPig
Type
Vitamins
Information
British Journal of Nutrition , Volume 62 , Issue 3 , November 1989 , pp. 781 - 789
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

Barth, C.A., Brigg, M. & Hagemeister, H. (1986). Biotin absorption from the hind gut of the pig. Journal of Animal Physiology and Animal Nutrition 55, 128–134.CrossRefGoogle Scholar
Chapman, D.I. & Marcroft, J. (1971). The use of Triton X-100 in the liquid scintillation counting of 14C with particular reference to plasma and urine. International Journal of Applied Radioactive Isotopes 22, 371–377.CrossRefGoogle Scholar
Hecker, J.F. (1974). Experimental Surgery on Small Ruminants. London: Butterworths.Google Scholar
Hood, R.L. (1977). Use of linear regression analysis in the isotope dilution assay of biotin. Analytical Biochemistry 79, 635–638.CrossRefGoogle ScholarPubMed
Kopinski, J.S. & Leibholz, J. (1989). Biotin studies in pigs. 2. The biotin requirement of the growing pig. British Journal of Nutrition 62, 761–766.CrossRefGoogle ScholarPubMed
Kopinski, J.S., Leibholz, J. & Bryden, W.L. (1989 a). Biotin studies in pigs. 3. Biotin absorption and synthesis. British Journal of Nutrition 62, 767–772.CrossRefGoogle ScholarPubMed
Kopinski, J.S., Leibholz, J. & Bryden, W.L. (1989 b). Biotin studies in pigs. 4. Biotin availability in feedstuffs for pigs and chickens. British Journal of Nutrition 62, 773–780.CrossRefGoogle ScholarPubMed
Kopinski, J.S., Leibholz, J., Bryden, W.L. & Fogarty, A.C. (1989 c). Biotin studies in pigs. 1. Biotin deficiency in the young pig. British Journal of Nutrition 62, 751–759.CrossRefGoogle ScholarPubMed
McCormick, D.B. (1975). Biotin. Nutrition Reviews 33, 97–102.CrossRefGoogle ScholarPubMed
Sorrell, M.F., Frank, O., Thomson, A.D., Aquino, H. & Baker, H. (1971). Absorption of vitamins from the large intestine in vivo. Nutrition Reports International 3, 143–148.Google Scholar
Steel, R.G.D. & Torrie, J.H. (1980). Principles and Procedures of Statistics, 2nd ed. New York: McGraw Hill.Google Scholar
Tagwerker, F.J. (1978). Roche Information Service Bulletin no. 1675. Basle: Roche Products Ltd.Google Scholar
Takken, A. & Williams, K.C. (1981). A simplified procedure for long-term catheterisation of the anterior vena cava in adult pigs. Australian Veterinary Journal 57, 17–20.CrossRefGoogle ScholarPubMed