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Metallothionein gene identification and expression in the cockle (Cerastoderma edule) under parasitism (trematodes)and cadmium contaminations

Published online by Cambridge University Press:  17 May 2007

Céline Desclaux-Marchand ,
Ika Paul-Pont ,
Patrice Gonzalez ,
Magalie Baudrimont  and
Xavier de Montaudouin
Céline Desclaux-Marchand
Affiliation:
Station Marine d'Arcachon, Université Bordeaux 1 – UMR EPOC 5805, 2 rue du Pr Jolyet, 33120 Arcachon, France
Ika Paul-Pont
Affiliation:
Station Marine d'Arcachon, Université Bordeaux 1 – UMR EPOC 5805, 2 rue du Pr Jolyet, 33120 Arcachon, France
Patrice Gonzalez
Affiliation:
Station Marine d'Arcachon, Université Bordeaux 1 – UMR EPOC 5805, 2 rue du Pr Jolyet, 33120 Arcachon, France
Magalie Baudrimont
Affiliation:
Station Marine d'Arcachon, Université Bordeaux 1 – UMR EPOC 5805, 2 rue du Pr Jolyet, 33120 Arcachon, France
Xavier de Montaudouin
Affiliation:
Station Marine d'Arcachon, Université Bordeaux 1 – UMR EPOC 5805, 2 rue du Pr Jolyet, 33120 Arcachon, France
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Abstract

Among benthic organisms, bivalves are often used as bioindicators of environmental pollution because of their high bioaccumulation capacities for heavy metals leading to metallothioneins (MT) induction. MT are small cytosolic metal-binding proteins involved in metal homeostasis and detoxification in living organisms. These proteins can also be induced by a wide range of factors, such as hormones, physical stress, parasitism. MT quantification in relation to parasitism is rarely reported in literature, while parasites are omnipresent and have deleterious impacts on bivalves. Moreover, only a few number of MT genes have been characterized in molluscs. This study describes the partial sequence of the MT gene (Cemt1) in the edible cockle Cerastoderma edule. The cockle's MT cDNA was sequenced and showed over 80% homology to several other bivalve MT sequences. This sequence was then used to determine MT specific primers which can be used in quantitative real time PCR. MT protein and gene expression levels were quantified for individuals selected under different conditions: free from or infected by the digenean trematode Himasthla elongata, and under cadmium exposure at 15 µg Cd L−1. Results evidenced that MT concentrations were significantly increased by both treatments; parasite infection and Cd exposure. Moreover, congruent results between MT protein and gene expression levels were obtained.

Keywords

TrematodesCocklesCadmiumMetallothioneinGene quantification
Type
Research Article
Information
Aquatic Living Resources , Volume 20 , Issue 1: New developments in coastal environment research: results from the national coastal environment program , January 2007 , pp. 43 - 49
Copyright
© EDP Sciences, IFREMER, IRD, 2007

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References

Achard-Joris, M., Gonzalez, P., Marie, V., Baudrimont, M., Bourdineaud, J.P., 2006, Cytochrome c oxydase subunit I gene is up-regulated by cadmium in freshwater and marine bivalves. Biometals 19, 237-244. CrossRef
Amiard, J.C., Amiard-Triquet, C., Barka, S., Pellerin, J., Rainbow, P.S., 2006, Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers. Aquat. Toxicol. 76, 160-202. CrossRef
Baudrimont, M., de Montaudouin, X., 2007, Evidence of an altered protective effect of metallothioneins after acdmium exposure in the digenean parasite-infected cockle (Cerastoderma edule). Parasitology 134, 237-245. CrossRef
Baudrimont, M., de Montaudouin, X., Palvadeau, A., 2006, Impact of digenean parasites infection on metallothionein synthesis by the cockle (Cerastoderma edule): a multivariate field monitoring. Mar. Pollut. Bull. 52, 494-502. CrossRef
Baudrimont, M., Andrès, S., Durrieu, G., Boudou, A., 2003, The key role of metallothioneins in the bivalve Corbicula fluminea during the depuration phase, after in situ exposure to Cd and Zn. Aquat. Toxicol. 63, 89-102. CrossRef
Baudrimont, M., Lemaire-Gony, S., Ribeyre, F., Métivaud, J., Boudou, A., 1997, Seasonal variations of metallothioein concentrations in the asiatic clam (Corbicula fluminea). Comp. Biochem. Physiol. 118C, 361-367.
Baudrimont, M., Andrès, S., Metivaud, J., Lapaquellerie, Y., Ribeyre, F., Maillet, N., Latouche, C., Boudou, A., 1999, Field transplantation of the freshwater bivalve Corbicula fluminea along a polymetallic contamination gradient (river Lot, France): II. Metallothionein response to metal exposure. Environ. Toxicol. Chem. 18, 2472-2477.
Bowers, E.A., 1969, Cercaria bucephalopsis haimeana (Lacaze-Duthiers, 1854) (Digenea: Bucephalidae) in the cockle, Cardium edule L. in South Wales. J. Nat. Hist. 3, 409-422. CrossRef
Chan, J., Huang, Z., Merrifield, M.E., Salgado, M.T., Stillman, M.J., 2002, Studies of metal binding reactions in metallothioneins by spectroscopic, molecular biology, and molecular modeling techniques. Coord. Chem. Rev. 233-234, 319-339. CrossRef
Cheggour, M., Chafik, A., Langston, W.J., Burt, G.R., Benbrahim, S., Texier, H., 2001, Metals in sediments and the edible cockle Cerastoderma edule from two Moroccan Atlantic lagoons: Moulay Bou Selham and Sidi Moussa. Environ. Pollut. 115, 149-160. CrossRef
Curtis, L.A., Hubbard, K.M., 1990, Trematode infections in a gastropod host misrepresented by observing shed cercariae. J. Exp. Mar. Biol. Ecol. 143, 131-137. CrossRef
Delnomdedieu, M., Boudou, A., Geordescauld, D., Dufourc, E.J., 1992, Specific interactions of mercury chloride with membranes and other ligands as revealed by mercury-NMR. Chem. Biol. Interact. 81, 243-269. CrossRef
Dutton, M.D., Stephenson, M., Klaverkamp, J.F., 1993, A mercury saturation assay for measuring metallothionein in fish. Environ. Toxicol. Chem. 12, 1193-1202. CrossRef
de Montaudouin, X., Kisielewski, I., Bachelet, G., Desclaux, C., 2000, A census of macroparasites in an intertidal bivalve community, Arcachon Bay, France. Oceanol. Acta 23, 453-468. CrossRef
de Montaudouin X., Blanchet H., Kisielewski I., Desclaux C., Bachelet G., 2003, Digenean trematodes moderately alter Hydrobia ulvae population size structure. J. Mar. Biol. Assoc. UK 297-305.
Desclaux, C., de Montaudouin, X., Bachelet, G., 2002, Cockle emergence at the sediment surface: “favourization” mechanism by digenean parasites? Dis. Aquat. Org. 52, 137-149. CrossRef
Desclaux, C., de Montaudouin, X., Bachelet, G., 2004, Cockle (Cerastoderma edule) population mortality: the role of the digenean parasite Himasthla quissetensis. Mar. Ecol. Prog. Ser. 279, 141-150. CrossRef
Desclaux, C., Russell-Pinto, F., de Montaudouin, X., Bachelet, G., 2006, First record and description of metacercariae of Curcuteria arguinae n. sp. (Digenea: Echinostomatidae), parasite of cockles Cerastoderma edule (Mollusca: Bivalvia) in Arcachon Bay, France. J. Parasitol. 92, 578-587. CrossRef
Gonzalez, P., Baudrimont, M., Boudou, A., Bourdineaud, J.P., 2006, Comparative effects of direct cadmium contamination on gene expression in gills, skeletal muscles and brain of the zebrafish (Danio rerio). Biometals 19, 225-235. CrossRef
Griscom, S.B., Fisher, N.S. and Luoma, S.N., 2002, Kinetic modeling of Ag, Cd and Co bioaccumulation in the clam Macoma balthica: quantifying dietary and dissolved sources. Mar. Ecol. Prog. Ser. 240, 127-141. CrossRef
Griscom, S.B., Fisher, N.S., 2004, Bioavailability of sediment-bound metals to marine bivalve molluscs: an overview. Estuaries 27, 826-838. CrossRef
Gundacker, C., 1999, Tissue-specific heavy metal (Cd, Pb, Cu, Zn) deposition in a natural population of the zebra mussel Dreissena polymorpha Pallas. Chemosphere 38, 3339-3356. CrossRef
Haas W., 2000, The behavioral biology of echinostomes. In: Fried, B., Graczyk, T.K. (Eds), Echinostomes as experimental models for biological research, Kluwer Academic Publishers, Dordrecht, pp. 175-197.
Jensen, K.T., Mouritsen, K.N., 1992, Mass mortality in two common soft-bottom invertebrates, Hydrobia ulvae and Corophium volutator – the possible role of trematodes. Helgol. Meeresunters. 46, 329-339. CrossRef
Konigsberg, M., Lopez-Diazguerrero, N.E., Bucio, L., Gutierez-Ruiz, M.C., 2001, Uncoupling effect of mercury chloride on mitochondria isolated from an hepatic cell line. J. Appl. Toxicol. 21, 323-329. CrossRef
Lauckner G., 1983, Diseases of mollusca: bivalvia. In: Kinne, O. (Ed) Diseases of mearine animals. Biologische Anstalt Helgoland, Hamburg, pp. 477-961.
Lecoeur, S., Videmann, B., Berny, P., 2004, Evaluation of metallothionein as a biomarker of single and combined Cd/Cu exposure in Dreissena polymorpha. Environ. Res. 94, 184-191.
Lemoine, S., Laulier, M., 2003, Potential use of the mRNA of a specific metallothionein isoform (MT-20) in mussel (Mytilus edulis) as a biomarker of cadmium contamination. Mar. Pollut. Bull. 46, 1450-1455.
Marie, V., Gonzalez, P., Baudrimont, M., Boutet, I., Moraga, D., Bourdineaud, J.P., Boudou, A., 2006, Metallothionein gene expression and protein levels in triploid and diploid oysters Crassostrea gigas after exposure to cadmium and zinc. Environ. Toxicol. Chem. 25, 412-418. CrossRef
Overnell, J., Good, M., Vasak, M., 1988, Spectroscopic studies on cadmium (II)- and cobalt (II)-substituted metallothionein from the crab Cancer pagurus. Eur. J. Biochem. 172, 171-177. CrossRef
Roesijadi, G., 1992, Metallothioneins in metal regulation and toxicity in aquatic animals. Aquat. Toxicol. 22, 81-114. CrossRef
Russell-Pinto, F., Gonçalves, J.F., Bowers, E., 2006, Digenean larvae parasitizing Cerastoderma edule (Bivalvia) and Nassarius reticulatus (Gastropoda) from Ria de Aveiro, Portugal. J. Parasitol. 92, 319-332. CrossRef
Stohs, S.J., Bagchi, D., 1995, Oxidative mechanisms in the toxicity of metal ions. Free Radic. Biol. Med. 18, 321-336. CrossRef
Sures, B., 2004, Environmental parasitology: relevancy of parasites in monitoring environmental pollution. Trends Parasitol. 20, 170-177. CrossRef
Tanguy, A., Mura, C., Moraga, D., 2001, Cloning of a metallothionein gene and characterization of two other cDNA sequences in the Pacific oyster Crassostrea gigas (CgMT1). Aquat. Toxicol. 55, 35-47. CrossRef
Thieltges, D.W., 2006, Parasite induced summer mortality in the cockle Cerastoderma edule by the trematode Gymnophallus choledochus. Hydrobiologia 559, 455-461. CrossRef
Wegeberg, A.M., de Montaudouin, X., Jensen, K.T., 1999, Effect of intermediate host size (Cerastoderma edule) on infectivity of cercariae of three Himasthla species (Echinostomatidae, Trematoda). J. Exp. Mar. Biol. Ecol. 238, 259-269. CrossRef