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Measurement of the effect of organic pollution on marine organisms: rapid determination of EROD induction using plate readers

Published online by Cambridge University Press:  15 January 1991

Daniel Grzebyk  and
François Galgani
Daniel Grzebyk
Affiliation:
IFREMER, Laboratoire Effets Biologiques des Nuisances, rue de l'île d'Yeu, BP n° 1049, 44037 Nantes Cedex 01, France
François Galgani
Affiliation:
IFREMER, Laboratoire Effets Biologiques des Nuisances, rue de l'île d'Yeu, BP n° 1049, 44037 Nantes Cedex 01, France
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Abstract

The purpose of this study was to adapt the ethoxyresorufin O-deethylase (EROD) assay to the microplate technique. In addition to showing the practical advantages related to the intrinsic properties of such systems, we wished to demonstrate their potential value for biological monitoring of contamination in natural environments. A simpliffed procedure was developed for the preparation and fluorimetric assay of EROD activity using only basic laboratory equipment. The use of plate readers allows measurements to be done rapidly and provides numerous advantages in comparison with traditional methods. Within the context of our study; this test could be substituted to advantage for the enzymelinked immunosorbent assay (ELISA).

Keywords

Mixed function oxidaseethoxyresorufin O-deethylasecytochromeenzymeplate readerorganic pollutionÉthoxyrésorufin O-deethylaselecteur de plaquesenzymesurveillance biologiqueenvironnement
Type
Research Article
Information
Aquatic Living Resources , Volume 4 , Issue 1 , January 1991 , pp. 53 - 59
Copyright
© IFREMER-Gauthier-Villars, 1991

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