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Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa

Published online by Cambridge University Press:  15 February 1998

Marc Suquet ,
Catherine Dreanno ,
Bruno Petton ,
Yvon Normant ,
Marie-Hélène Omnes  and
Roland Billard
Marc Suquet
Affiliation:
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP70, 29280 Plouzané, France
Catherine Dreanno
Affiliation:
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP70, 29280 Plouzané, France Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43 rue Cuvier, 75231 Paris, France
Bruno Petton
Affiliation:
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP70, 29280 Plouzané, France
Yvon Normant
Affiliation:
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP70, 29280 Plouzané, France
Marie-Hélène Omnes
Affiliation:
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP70, 29280 Plouzané, France
Roland Billard
Affiliation:
Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43 rue Cuvier, 75231 Paris, France
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Abstract

The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use for routine aquaculture practices.

Keywords

turbotPsetta maximaspermcryopreservationhatching
Type
Research Article
Information
Aquatic Living Resources , Volume 11 , Issue 1 , February 1998 , pp. 45 - 48
Copyright
© Elsevier, IRD, Inra, Ifremer, Cemagref, CNRS, 1998

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